Next Generation icIEF

icIEF technology for in-depth biopharmaceutical characterization

Recent developments of imaged capillary isoelectric focusing technology for in-depth biopharmaceutical characterization

Learn more about the reviews on the origins and 30-year evolution of icIEF technology by illustrating its continuous progression and expanding impact over time. You will learn how useful our critical reagents and capillary coatings, advanced icIEF fractionation, cutting-edge icIEF-MS online coupling, and novel applications for protein bio-interactions, are redefining icIEF analytical methodologies and broadening their applicability. These breakthroughs significantly enhance the characterization of complex therapeutic proteins, aiding researchers in mitigating challenges and setbacks during drug development and manufacturing. In describing these advances, we deliver into multidisciplinary concepts spanning biology, chemistry, instrument design, and workflow optimization, aiming to inspire further innovations and insights in this dynamic, rapidly evolving field

We thank Dr. David A. Michels and Dr. Feng Yang from Genentech,  Dr. Bo Yan from Beam Therapeutics and Professor Janusz Pawliszyn from University of Waterloo for sharing their great experiences and sharing their great insights on the use of the Traditional icIEF and our Next Generation icIEF Solutions.

Cysteine-linked ADC disitamab vedotin Characterisation

In-depth characterization of a cysteine-linked ADC disitamab vedotin by multiple LC-MS analysis methods and cutting-edge imaged capillary isoelectric focusing coupled with native mass spectrometry

Learn more about the researchers at Thermo Fisher Scientific, how they have introduced the cutting-edge icIEF-MS under near-native condition for in-depth characterization of cysteine-linked ADC disitamab vedotin, which was integrated with traditional online chromatography-MS approaches.

Although CE based separation techniques online coupling with MS were already applied in protein charge variant analysis, these approaches have limitations for cysteine-linked ADC analysis. For example, icIEF-MS under denaturing condition will break the non- convalent bindings between light chains and heavy chains; microfluidic chip-based CE/CZE-MS [reference 23] has the challenge in method bridging and transfer from development to QC; Sun et al. [reference 38]. published cIEF-MS under native condition was a good attempt, but better data quality could be obtained with higher mass spectral resolution and sensitivity.

Therefore, icIEF-MS under near-native condition we developed in this work can keep the cysteine-linked ADC stay native during the icIEF separation and MS detection, also enables easily seamless icIEF method transfer from development to QC. This workflow underlined the outstanding importance of comprehensive separation techniques based on MS technology in the development of ADCs.

In-depth characterisation of mAbs & ADC charge variants with icIEF-HRAM MS

Learn more about researchers at Thermo Fisher Scientific how they use the CEInfinite icIEF-Preparative and online intact icIEF-MS for in depth characterization of mAbs, ADC charge variants and peptide mapping without extra interface. The CEInfinite simply your research by providing you consistency, repeatability and building platform methods . 

In-depth Characterisation of Fusion Proteins

Learn more about researchers at National Institutes for Food and Drug Control, who has developed a workflow integrating UHPLC-MS and CEInfinite icIEF-MS provided an innovative strategy for characterizing complex fusion proteins in the process of quality and manufacturing. 

New publication

                                Monoclonal antibody                                  Bispecific antibody                                       Fusion Proteins