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MS study on SARS-CoV-2 recombinant vaccine with comprehensive separation techniques to characterise complex heterogeneity

Gang Wu, Jialiang Du, Chuanfei Yu, et al. 2024

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iCIEF tandem high-resolution MS using nano electrospray ionization (ESI) for protein heterogeneity characterization 

Teresa Kwok, She Lin Chan, Matthew Courtney, Mike Zhou, Tiemin Huang, Tao Bo, Victor Li, Tong Chen. Analytical Biochemistry 680 (2023).

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Perspectives on Capillary Electrophoresis 

Barry L. Karger and James L. Waters. Trends in Analytical Chemistry. 

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Capillary electrophoresis-MS for intact protein analysis: Pharmaceutical and biomedical applications (2018-2023)

Katarina Marakova, Martina Opetova, Radovan Tomasovsky. Journal Separation Science 2023, 46

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Performance of capillary electrophoresis instruments - State of the art and outlook 

Sophie Hartung, Rober Minkner, Mais Olabi and Hermann Watzig. 2023. Trends in Analytical Chemistry 163; 117056

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iCIEF employing fluorocarbon and methylcellulose coated fused silica capillary for characterization of charge heterogeneity of protein biopharmaceuticals

Teresa Kwok, She Lin Chan, Jessica Shi, Mike Zhou et al. 2023

Sep Sci Plus

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Imaged capillary isoelectric focusing (iCIEF) tandem high-resolution mass spectrometry for charged heterogeneity of protein drugs in biopharmaceutical discovery

Xiaoxi Zhang, Teresa Kwok, Mike Zhou, Min Du,  Victor Li, Tao Bo, Tiemin Huang, Tong Chen
Journal of Pharmaceutical and Biomedical Analysis 224 (2023) 115178



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Cutting-edge mass spectrometry strategy based on imaged capillary isoelectric focusing (icIEF) technology for characterizing charge heterogeneity of monoclonal antibody
Xiaoxi Zhang, Tong Chen, Victor Li, Tao Bo, Min Du, Tiemin Huang
Analytical Biochemistry, Volume 660, 1 January 2023, 114961




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White paper: Critical reagents for successful iCIEF

Advanced Electrophoresis Solutions Ltd., October, 2022





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Characterization of therapeutic mAb charge heterogeneityby iCIEF coupled to mass spectrometry (iCIEF–MS)

Johannes Schlecht, Bernd Moritz, Steffen Kiessig, Christian Neusüß September23, 2022




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Global characterization of modifications to the charge isomers of IgG antibody

Xinling Cui, Wei Mi, Zhishang Hu, Xiaoyu Li, Bo Meng, Xinyuan Zhao,Xiaohong Qian, Tao Zhu, Wantao Ying
Journal of Pharmaceutical Analysis, 12 (2022) 156-163




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High-Efficiency Preparative Imaged Capillary Isoelectric Focusing (iCIEF) and iCIEF-MS Protein Charge Variant Characterisation

Tong Chen, Teresa Kwok, Anne Rose Esmin, Victor Li, Gerard Rozing,Tiemin Huang
Chromatography, May / June 2021




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High-Throughput Precision with On-Board Mixing for Imaged Capillary Isoelectric Focusing (iCIEF)

Katelyn Cousteils, Jennifer Hu, Victor Li, Tiemin Huang Chromatography, February / March 2019






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Coupling imaged capillary isoelectric focusing with mass spectrometry using a nanoliter valve

Faculty of Chemistry, Aalen University, Aalen, Germany
Electrophoresis, 2018DOI 10.1002/elps.201800013





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Determination of the isoelectric point of shortened glucagon-like peptide-1 by capillary isoelectric focusing with whole column imaging

Junjian Fang*, Jiexin Liu*, Haijing Li, Jianhua Cheng, Shengming Wu, and Fangting Dong





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Mass Spectrometry-Based Charge Heterogeneity Characterization of Therapeutic mAbs with Imaged Capillary Isoelectric Focusing and Ion-Exchange Chromatography as Separation Techniques - W. Gang, Y. Chuanfei, W. Wang et al. 2023

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