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An icIEF Separation of the Linear and Cyclic Variants of a Mimotope of the Cancer-Related CD20 Antigen–Validation and Statistical Evaluation

Georg Bloderer, Luigi Grassi, Chiara Cabrele and Hanno Stutz, 2025.

In depth-characterisation of a cysteine-linked ADCby LC-MS

Gang Wu, et al. 2024.

icIEF and online icIEF-MS for milk whey protein characterization in dairy products

She Lin Chan^a, Teresa Kwok^a, Niusheng Xu^a, Tao Bo^a,*, Tiemin Huang^a,b,**

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icIEF and MS for In-Depth Characterisation of Therapeutic Protein Charge Heterogeneity

Matthew Courtney, Mark Lies, Teresa Kwork et al. 2024

Characterisation of monocloncal antibodies and antibody drug conjugates using icIEF-MS online coupling platform

Xiaoxi Zhang, Tiemin Huang, Tony Chen, Tao Bo, Min Du. 2022

icIEF-MS Online Coupling for polatuzumab vedotin charge heterogeneity analysis using native MS

Xiaoxi Zhang, Tony Chen, Tao Bo, Tiemin Huang, Min Du. 2024

In Depth Characterisation of mAbs Charge Variants with icIEF Fractionation Followed by Peptide Mapping Analysis

Xiaoxi Zhang, Zhigui Zhou, Min Sang, Tiemin Huang et al. 2024

Integrating UHPLC tandem MS and icIEF for in-depth characterisation of complex fusion proteins.

Wenhong Fan, Xiang Li, Zhen Long et al. (2023)

MS study on SARS-CoV-2 recombinant vaccine with comprehensive separation techniques to characterise complex heterogeneity

Gang Wu, Jialiang Du, Chuanfei Yu, et al. 2024

iCIEF tandem high-resolution MS using nano electrospray ionization (ESI) for protein heterogeneity characterization

Teresa Kwok, She Lin Chan, Matthew Courtney, Mike Zhou, Tiemin Huang, Tao Bo, Victor Li, Tong Chen. Analytical Biochemistry 680 (2023).

Perspectives on Capillary Electrophoresis

Barry L. Karger and James L. Waters. Trends in Analytical Chemistry.

Capillary electrophoresis-MS for intact protein analysis: Pharmaceutical and biomedical applications (2018-2023)

Katarina Marakova, Martina Opetova, Radovan Tomasovsky. Journal Separation Science 2023, 46

Performance of capillary electrophoresis instruments - State of the art and outlook

Sophie Hartung, Rober Minkner, Mais Olabi and Hermann Watzig. 2023. Trends in Analytical Chemistry 163; 117056

iCIEF employing fluorocarbon and methylcellulose coated fused silica capillary for characterization of charge heterogeneity of protein biopharmaceuticals

Teresa Kwok, She Lin Chan, Jessica Shi, Mike Zhou et al. 2023

Sep Sci Plus

Cutting-edge mass spectrometry strategy based on icIEF technology for characterizing charge heterogeneity of monoclonal antibody
Xiaoxi Zhang, Tong Chen, Victor Li, Tao Bo, Min Du, Tiemin Huang
Analytical Biochemistry, Volume 660, 1 January 2023, 114961

iCIEF tandem high-resolution MS for charged heterogeneity of protein drugs in biopharmaceutical discovery

Xiaoxi Zhang, Teresa Kwok, Mike Zhou, Min Du, Victor Li, Tao Bo, Tiemin Huang, Tong Chen
Journal of Pharmaceutical and Biomedical Analysis 224 (2023) 115178

Characterization of therapeutic mAb charge heterogeneityby iCIEF–MS

Johannes Schlecht, Bernd Moritz, Steffen Kiessig, Christian Neusüß September23, 2022

White paper: Critical reagents for successful iCIEF

Advanced Electrophoresis Solutions Ltd., October, 2022

Global characterization of modifications to the charge isomers of IgG antibody

Xinling Cui, Wei Mi, Zhishang Hu, Xiaoyu Li, Bo Meng, Xinyuan Zhao,Xiaohong Qian, Tao Zhu, Wantao Ying
Journal of Pharmaceutical Analysis, 12 (2022) 156-163

High-Efficiency Preparative Imaged Capillary Isoelectric Focusing (iCIEF) and iCIEF-MS Protein Charge Variant Characterisation

Tong Chen, Teresa Kwok, Anne Rose Esmin, Victor Li, Gerard Rozing,Tiemin Huang
Chromatography, May / June 2021

High-Throughput Precision with On-Board Mixing for Imaged Capillary Isoelectric Focusing (iCIEF)

Katelyn Cousteils, Jennifer Hu, Victor Li, Tiemin Huang Chromatography, February / March 2019

Coupling imaged capillary isoelectric focusing with mass spectrometry using a nanoliter valve

Faculty of Chemistry, Aalen University, Aalen, Germany
Electrophoresis, 2018, DOI 10.1002/elps.201800013

Determination of the isoelectric point of shortened glucagon-like peptide-1 by capillary isoelectric focusing with whole column imaging

Junjian Fang*, Jiexin Liu*, Haijing Li, Jianhua Cheng, Shengming Wu, and Fangting Dong
INSTRUMENTATION SCIENCE AND TECHNOLOGY, 2018

Mass Spectrometry-Based Charge Heterogeneity Characterization of Therapeutic mAbs with Imaged Capillary Isoelectric Focusing and Ion-Exchange Chromatography as Separation Techniques

W. Gang, Y. Chuanfei, W. Wang et al. 2023

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