The high quality RNAdia kit allows for the construction of sequencing ready libraries. Those libraries are compatible with standard NGS short-read sequencing on Illumina sequencers, allowing analyses of thousands of transcriptional profiles of single cells in parallel.
This kit consist of all reagents and consumables needed for RNA-Sequencing with the Nadia Instrument. The reagents are divided in step by step boxes. A complete workflow from encapsulation to library preparation, with option for bioinformatic bundle in the future. The kit consist the new beads for high gene capture at low sequencing depth.
With this kit, Single cell sequencing can be performed at half costs. This way more samples can be measured, encouraging research.
The LUTHOR kit provides an easy one step solution for 3’ mRNA-Seq library preparation. With high sensitivity and reproducibility of the kit, the best results will be obtained. The LUTHOR RNA-Seq kit can handle both RNA and (frozen) single cell samples. Ultra-low amounts of RNA (10-100 pg) read up to 13.000-16.000 genes. For single cells 6000-12.500 genes can be detected at 1M reads per sample.
With the LUTHOR RNA-Seq kit, fast data processing is a standard. It represents endogenous mRNA composition and excludes ribosomal rRNAs. There will be only focussed on the coding sequences. Read mapping is simplified by reads of the transcript 3’end of the strand, therefore excluding any junction detection.
With a strand-specificity of >99.9% it allows to map reads to their corresponding strand on the genome, enabling the discovery and quantification of antisense transcripts and overlapping genes.
One fragment per amplified antisense RNA copy is produced. Therefore, no length normalisation is required. This provides straightforward determination of gene expression values.
The LUTHOR RNA-Seq kit uses Lexogen’s THOR technology for direct RNA amplification. The Luther RNA-Seq kit is intended to be used with Unique Dual Indices (UDIs), Unique Dual Indexing Sets (Cat. No. 101 – 105, 156). LUTHOR is also designed to yield insert sizes suitable for Single Read (SR) 50 to SR150