LUTHOR 3’mRNA-Seq Library Prep Kit
The LUTHOR kit provides an easy one-step solution for Single Cell 3’ mRNA Sequencing library preparation. With high sensitivity and reproducibility of the kit, the best results will be obtained.
- Processing of both RNA and (frozen) single-cell samples.
- Ultra-low amounts of RNA (10-100 pg) read up to 13.000-16.000 genes.
- For single cells 6000-12.500 genes can be detected at 1M reads per sample.
With the LUTHOR RNA-Seq kit, fast data processing is standard. It represents endogenous mRNA composition and excludes ribosomal rRNAs. There will be only focused on the coding sequences. Read mapping is simplified by reads of the transcript 3’end of the strand, therefore excluding any junction detection.
With a strand-specificity of >99.9% it allows mapping reads to their corresponding strand on the genome, enabling the discovery and quantification of antisense transcripts and overlapping genes.
One fragment per amplified antisense RNA copy is produced. Therefore, no length normalisation is required. This provides straightforward determination of gene expression values.