Single Cell Research

RNAdia reagents kit

Single cell research doesn‘t need to be expensive

RNAdia – 50% less than competitor reagents 

The high quality RNAdia kit allows for the construction of sequencing ready libraries. Those libraries are compatible with standard NGS short-read sequencing on Illumina sequencers, allowing analyses of thousands of transcriptional profiles of single cells in parallel.

RNAdia reagents kit

This kit consist of all reagents and consumables needed for RNA-Sequencing with the Nadia Instrument. The reagents are divided in step by step boxes. A complete workflow from encapsulation to library preparation, with option for bioinformatic bundle in the future. The kit consist the new beads for high gene capture at low sequencing depth.

With this kit, Single cell sequencing can be performed at half costs. This way more samples can be measured, encouraging research.

  • Cell and bead suspension buffer
  • Barcode beads
  • Emulsion oil
  • Perfluorooctanol
  • Bead wash buffer
  • Reversed transcription reaction mix
  • Exonuclease reaction mix
  • PCR reaction mix
  • Consumables

Agarose encapsulation for live cell studies

We offer also separate kits for agarose encapsulation and 3D cells formation. The nadAROSE kit produces agarose scaffolds that can be later disgested to release cells. The kit creates a spherical scaffold structure to allow diffusion of nutrients and gases that make it an ideal system for naturalistic mammalian and non-mammalian live cell studies. Besides single cell, there is a possibility for multiple cell encapsulation, so cell-cell or cell-pathogen interactions can be analysed. Other applications are cell secretion, microbial cell culture, cytometric analysis.

 

Collagen encapsulation for 3D cell growth

The nadia3D kit produces collagen-based scaffolds that can be later digested to release cells. The collagen scaffolds promote cell growth in a 3D physiologically relevant manner. Encapsulated cells were cultured and observed for 7 days. Collagen-based scaffolds compatible with cell release due to scaffold's dissolution without the loss of cells

  • Enables 3D cell culture growth 
  • Maintains single cell identity
  • Applicable to all kind of cell lines, tumors or single cell suspensions
  • Up to 1 mill. cells in one run

RNAdia2.0

NEW! Get an easy, accessible and simple Single Cell Research workflow with our new version of the RNAdia 2.0 kit for the Nadia Instrument.  

  • Save time with shorter protocols 
  • Get reproducible and consistent high-quality results 
  • Save costs with a lower price comparatively. 
  • Analyze more genes per cell with a median number of 1000 genes/cell.
  • Automated: separation of droplets with/without bead

The Droplet Analyser Software (DAS) measures the beads encapsulation rates. It monitors single-cell encapsulation rates to minimize empty or double-filled droplets.

Generate Single Cell RNA-sequencing libraries: 

  • Encapsulate thousands of single cells in <30 minutes. 
  • Make sure your samples stay in suspension during the run. 
  • It enables the study of cellular expression profiles 

Read the Application Note  

LUTHOR 3’mRNA-Seq Library Prep Kit

The LUTHOR kit provides an easy one step solution for 3’ mRNA-Seq library preparation. With high sensitivity and reproducibility of the kit, the best results will be obtained. The LUTHOR RNA-Seq kit can handle both RNA and (frozen) single cell samples. Ultra-low amounts of RNA (10-100 pg) read up to 13.000-16.000 genes. For single cells 6000-12.500 genes can be detected at 1M reads per sample.

With the LUTHOR RNA-Seq kit, fast data processing is a standard. It represents endogenous mRNA composition and excludes ribosomal rRNAs. There will be only focussed on the coding sequences. Read mapping is simplified by reads of the transcript 3’end of the strand, therefore excluding any junction detection.

With a strand-specificity of >99.9% it allows to map reads to their corresponding strand on the genome, enabling the discovery and quantification of antisense transcripts and overlapping genes.

One fragment per amplified antisense RNA copy is produced. Therefore, no length normalisation is required. This provides straightforward determination of gene expression values.

The LUTHOR RNA-Seq kit uses Lexogen’s THOR technology for direct RNA amplification. The Luther RNA-Seq kit is intended to be used with Unique Dual Indices (UDIs), Unique Dual Indexing Sets (Cat. No. 101 – 105, 156). LUTHOR is also designed to yield insert sizes suitable for Single Read (SR) 50 to SR150

Benefits of the LUTHOR 3'mRNA kit.

  • Direct RNA amplification
  • Empower challenging samples
  • High sensitivity

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support@isogen-lifescience.com

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