Single Cell Research

RNAdia reagents kit

Single cell research doesn‘t need to be expensive

RNAdia – 50% less than competitor reagents 

The high quality RNAdia kit allows for the construction of sequencing ready libraries. Those libraries are compatible with standard NGS short-read sequencing on Illumina sequencers, allowing analyses of thousands of transcriptional profiles of single cells in parallel.

This kit consist of all reagents and consumables needed for RNA-Sequencing with the Nadia Instrument. The reagents are divided in step by step boxes. A complete workflow from encapsulation to library preparation, with option for bioinformatic bundle in the future. The kit consist the new beads for high gene capture at low sequencing depth.

With this kit, Single cell sequencing can be performed at half costs. This way more samples can be measured, encouraging research.

• Cell and bead suspension buffer
• Barcode beads
• Emulsion oil
• Perfluorooctanol
• Bead wash buffer
• Reversed transcription reaction mix
• Exonuclease reaction mix
• PCR reaction mix
• Consumables

Agarose encapsulation for live cell studies

We offer also separate kits for agarose encapsulation and 3D cells formation. The nadAROSE kit produces agarose scaffolds that can be later disgested to release cells. The kit creates a spherical scaffold structure to allow diffusion of nutrients and gases that make it an ideal system for naturalistic mammalian and non-mammalian live cell studies. Besides single cell, there is a possibility for multiple cell encapsulation, so cell-cell or cell-pathogen interactions can be analysed. Other applications are cell secretion, microbial cell culture, cytometric analysis.

Collagen encapsulation for 3D cell growth

The nadia3D kit produces collagen-based scaffolds that can be later digested to release cells. The collagen scaffolds promote cell growth in a 3D physiologically relevant manner. Encapsulated cells were cultured and observed for 7 days. Collagen-based scaffolds compatible with cell release due to scaffold's dissolution without the loss of cells

• Enables 3D cell culture growth 
• Maintains single cell identity
• Applicable to all kind of cell lines, tumors or single cell suspensions
• Up to 1 mill. cells in one run