Digital PCR (dPCR)

Name: Isogen Life Science B.V.
Address: Veldzigt 2a, De Meern, 3454 PW, NL
Telephone: +31 30 6880771

Absolute DNA/RNA Quantification

Digital Droplet PCR (ddPCR) is an advanced version of traditional PCR that allows for absolute quantification of nucleic acids (DNA or RNA) rather than just relative quantification.

Instead of amplifying DNA in a single reaction tube (like standard PCR), digital PCR partitions the sample into thousands or even millions of tiny reactions (droplets, wells, or chambers). This partitioning increases sensitivity, accuracy, and robustness, especially in applications requiring detection of low-abundance targets or rare genetic variants. As a result, dPCR has become an essential tool in areas such as oncology, infectious disease monitoring, and genetic research.

⭐ Researchers' Reviews!

VibroJect® technology: droplet formation without the need for microfluidic chips

This technology simplifies the workflow and reduces maintenance requirements:

Utilizes mechanical vibration to produce uniform droplets, eliminating the need for microfluidic chips.
Enhances droplet consistency and reduces system complexity, leading to improved accuracy and reliability in dPCR assays.

We offer two systems: DQ24 and DQ Infinity

sniper system digital pcr

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DQ24

Digital System is an all-in-one equipment – Press the start button and no more operations are needed. Full duration of the experiments is less than 2 hr.

All-in-One Automation: Integrates droplet generation, thermal cycling, and fluorescence detection into a single platform.
Multiplexing Capability: Supports up to 6-color fluorescence detection, enabling the simultaneous analysis of multiple targets.
Open System Design: Compatible with standard 8-well PCR strips and various reagents, offering flexibility in assay development.
Regulatory Compliance: Holds CE and FDA IVD certifications, ensuring adherence to international standards (e.g 21 CFR Part 11 for data integrity).
High Sensitivity and Precision: Capable of detecting low-abundance targets with high accuracy, making it suitable for applications such as oncology, infectious disease detection, and gene therapy research.

DQ Infinity

High-Throughput Processing: Designed for large-scale applications, providing fully automated, high-throughput dPCR analysis.

Applications include:

Oncology Biomarker Detection: Detection of cancer biomarkers and monitoring of minimal residual disease.
Infectious Diseases: Quantification of viral and bacterial pathogens.
Cell and Gene Therapy: Assessment of vector copy numbers and gene expression levels.
Environmental Monitoring: Detection of microbial contaminants in water and soil samples.
Gene Expression Analysis: Reproducible measurement of transcripts of low expression or small sequence difference, such as single cell gene expression or allele-specific expression.
Food Safety Inspection
Environmental monitoring

Innovation, Opportunity, and a New Landscape for Molecular Diagnostics

"Two useful examples come from Suzhou Sniper Medical Technologies. One is its DQ24 Plus Digital PCR System, which I have recently installed in my own laboratory. The platform directly addresses one of digital PCR’s long-standing practical weaknesses: workflow complexity.

Traditional digital PCR systems often require separate instruments for droplet generation, thermal cycling, and fluorescence reading, with multiple manual handling steps between stages. The DQ24 Plus integrates droplet generation, amplification, and fluorescence detection within a single instrument.

Hands-on time is minimal, and results are available in approximately 90 minutes. Based on my own experience in rapid PCR development, I suspect that carefully optimised assays and reaction conditions may reduce effective turnaround times further.

nother important advantage is flexibility. Users are not confined to proprietary reagents. The ability to run independently developed assays and selected chemistries can significantly reduce per-reaction costs, removing a major barrier to routine or higher-throughput applications." - Stephen Bustin ⭐⭐⭐⭐

Read the full article here: https://www.stephenbustin.com/commentary

Why Isogen Life Science?

At Isogen Life Science, we believe that long term relationships with life science professionals and suppliers are fundamental to solve life science problems. Our curiosity drives us to continually stay involved and to dig deep in dialogue, with both clients and suppliers. It’s these intensive collaborations that enable us to develop tailored, innovative products that push life science’s boundaries. Partner up with us and enjoy our quick support for troubleshooting and questions!