Next Generation icIEF

Imaging Capillary Isoelectric Focusing Solutions

Since 2004, Isogen Life Science is the exclusive distributor of the CEInfinite Imaging Capillary Isoelectric Focusing (icIEF) product line from AES (Advanced Electrophoresis Solutions Ltd) for the European market. 

Our icIEF and capillary electrophoresis (CE) system gives you an in-depth protein insight. Besides the instrumentation, we offer a broad range of ampholytes, pI Markers, special coated cartridges for your utmost flexible method development.

For Capillary Electrophoresis (CE) Solutions, we offer the CAPEL CE instruments. covering a complete icIEF and CE Solutions.

CEInfinite icIEF Instruments

The CEInfinite is a next generation multifunctional platform with a whole column imaging detection (WCTID) automated cIEF system, standard equipped with a high sensitivity CMOS imaging sensor and powerful deep UV LED light source. All this, enables us to provide systems capable of increasing scan rate over 100-fold compared to any other (i)cIEF systems on the market. 

It enables the study of fast dynamic processes more accurately, higher sensitivity, empowering Taylor dispersion, rapid measurement of protein hydrodynamic radius, and protein interaction studies.

Our CEInfinite Chromeleon Driver integration allows you to work in a well control laboratory environment and streamlining workflow while meeting 21 CFR Part 11 compliance. We are in the process of developing Empower integration as well.

Furthermore, thanks to our patented Capillary Diameter Transformation Technique (CDTT), iCIEF systems enable you to perform direct coupling to MS for in-depth characterization studies and high-resolution icIEF fractionation for affinity, SPR, ELISA, peptide mapping,  and many more biological assays.

CEInfinite icIEF benefits:

Fast Protein Charge Analysis

CEInfinite dramatically increases the scan rate over 100 times compared to any other imaging time-resolved cIEF systems.

  • Fast protein charge analysis can be performed with the automated CEInfinite icIEF-Analytical instrument
  • The physical separation of the charge variants, structural and functional analyses are done by icIEF-Prep and/or online icIEF-MS.
  • Thanks to the long and small transfer capillary, the automated CEInfinite icIEF-Prep instrument enables you to spot the separated protein elution to a MALDI target plate, SPR plate or others.

 Customer Reviews                             

Latest Publications 

      Webinars         

 

Cysteine-linked ADC disitamab vedotin Characterisation

In-depth characterization of a cysteine-linked ADC disitamab vedotin by multiple LC-MS analysis methods and cutting-edge imaged capillary isoelectric focusing coupled with native mass spectrometry

Learn more about the researchers at Thermo Fisher Scientific, how they have introduced the cutting-edge icIEF-MS under near-native condition for in-depth characterization of cysteine-linked ADC disitamab vedotin, which was integrated with traditional online chromatography-MS approaches.

in-depth characterisation of a cysteine-linked ADC disitamab vedotin

 

Although CE based separation techniques online coupling with MS were already applied in protein charge variant analysis, these approaches have limitations for cysteine-linked ADC analysis. For example, icIEF-MS under denaturing condition will break the non- convalent bindings between light chains and heavy chains; microfluidic chip-based CE/CZE-MS [reference 23] has the challenge in method bridging and transfer from development to QC; Sun et al. [reference 38]. published cIEF-MS under native condition was a good attempt, but better data quality could be obtained with higher mass spectral resolution and sensitivity.

Therefore, icIEF-MS under near-native condition we developed in this work can keep the cysteine-linked ADC stay native during the icIEF separation and MS detection, also enables easily seamless icIEF method transfer from development to QC. This workflow underlined the outstanding importance of comprehensive separation techniques based on MS technology in the development of ADCs.

In-depth characterisation of mAbs & ADC charge variants with icIEF-HRAM MS

Learn more about researchers at Thermo Fisher Scientific how they use the CEInfinite icIEF-Preparative and online intact icIEF-MS for in depth characterization of mAbs, ADC charge variants and peptide mapping without extra interface. The CEInfinite simply your research by providing you consistency, repeatability and building platform methods . 

 

In-depth Characterisation of Fusion Proteins

Learn more about researchers at National Institutes for Food and Drug Control, who has developed a workflow integrating UHPLC-MS and CEInfinite icIEF-MS provided an innovative strategy for characterizing complex fusion proteins in the process of quality and manufacturing. 

Learn more about researchers at National Institutes for Food and Drug Control,

New publication

MS study on SARS-CoV recombinant vaccine with comprehensive separation techniques to characterise complex heterogeneity. 

In this study, our CEInfinite icIEF enables the fractionation of all 18 peaks into 5 groups based on their pI values and collected fractions from each group were for offline SCX-MS characterization. Identified peaks from 1 to 4 using icIEF-MS online workflow are missed in the SCX-MS identification. A simple explanation for this is that our icIEF-MS has better sensitivity than SCX-MS due to icIEF sample preconcentration and a consistent nano-flow rate mobilisation.

MS study on SARS-CoV-2-recombinant vaccine with comprehensive separation techniques to characterize complex heterogeneity

 

 

 

 

 

 

 

 

 

 

 

 

The separation mechanism of icIEF and IEX is different from each other. Both are broadly used in biotherapeutics charge variant analysis, by combining them together, you can collect more complementary information.

The separation mechanism of icIEF and IEX is different from each other. Both are broadly used in biotherapeutics charge variant analysis, by combining them together

 

 

 

 

 

 

 

 

 

 

 

 

Our CEInfinite icIEF-MS technology makes the protein heterogeneity characterization more straightforward, sensitive, high throughput and high data consistent.

The icIEF-MS online can be used for rapidly fingerprinting the complex heterogeneity of recombinant vaccine.

The icIEF fractionation is for simplifying the complexity of the protein samples, that allows you to carry out in-depth and more accurate characterization of collected fractions by HPLC-MS, and IEX-MS.

 

Read the publication here

Other icIEF Assays

Our assay methods coverage include:

  • Monoclonal antibodies (mAb), bispecific mAbs, and antibody drug conjugates (ADC)
  • Fusion proteins, adeno-associated viruses (AAV), viral proteins
  • Messenger RNAs (mRNA)
  • Lipid nanoparticles (LNP)
  • Enzymes
  • Exosomes, 
  • and more!

                 Monoclonal antibody                                        Bispecific antibody                                        Fusion Proteins

Our assay methods covere mAb, bispecific mAb, ADC,  fusion protein,AAV , mRNA, LNPs, enzyme, virus proteins and many more.

Special Offer: Placement of a new icIEF and/or

Placement of icIEF-Prep for all icIEF assays

 

chromeleon compatibility

  • icIEF-Analytical instrument: Profiling your proteins on pI values
  • icIEF-Prep Placement benefits:
    • Profiling your proteins on pI values.
    • Fractionation: High resolution of 0.1 pI differences and fast sample runs <45 minutes.
    • icIEF-MS direct coupling: Intact analysis of charge variants and more. One run <45 minutes

*Summer Offer, valid until the 21st September 2024.

Customer Reviews

Customer Review: icIEF in Pharmaceutics

"icIEF plays a critical for monitoring charge variants during biopharmaceutical development. The CEInfinite allows us to get a mass readout for the product variants detected by icIEF, and this is a great help for understanding the chemical nature of the charge variants.

When combining the CEInfinite with high resolution Orbitrap MS, detailed insight into product variants and product stability can be achieved.

In short, icIEF MS using the CEInfinite is a very powerful characterisation tool in biopharmaceutical development.”

Dan Bach Krinstensen (PhD - Principal Scientist)

icIEF-MS for Proteofroms

"The CEInfinite enables direct coupling of icIEF to mass spectrometry, which is a powerful tool for the characterisation of proteoforms on the intact level.
The obtained high resolution mass spectra are of excellent quality - For me a surprisingly high quality even for intact mAbs although we use generic separation methods (standard ampholytes)."

Prof. Dr. Christian Neusüß (Hochschule Aelan)

Quick and Easy icIEF

"CEInfinite iCIEF instrument enables us to verify easily and quickly the behavior of the pI markers we have proposed.

The operation with the instrument is easy and the time for the separation in the order of minutes, while the same focusing task performed in the classical CE capillary equipment with pressure mobilization took hours."

Professor Bohuslav Gas (Charles University, Faculty of Science)

Read his article: CE determination of the thermodynamic pKa

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