Next Generation icIEF

Cysteine-linked ADC disitamab vedotin Characterisation

In-depth characterization of a cysteine-linked ADC disitamab vedotin by multiple LC-MS analysis methods and cutting-edge imaged capillary isoelectric focusing coupled with native mass spectrometry

Learn more about the researchers at Thermo Fisher Scientific, how they have introduced the cutting-edge icIEF-MS under near-native condition for in-depth characterization of cysteine-linked ADC disitamab vedotin, which was integrated with traditional online chromatography-MS approaches.

Although CE based separation techniques online coupling with MS were already applied in protein charge variant analysis, these approaches have limitations for cysteine-linked ADC analysis. For example, icIEF-MS under denaturing condition will break the non- convalent bindings between light chains and heavy chains; microfluidic chip-based CE/CZE-MS [reference 23] has the challenge in method bridging and transfer from development to QC; Sun et al. [reference 38]. published cIEF-MS under native condition was a good attempt, but better data quality could be obtained with higher mass spectral resolution and sensitivity.

Therefore, icIEF-MS under near-native condition we developed in this work can keep the cysteine-linked ADC stay native during the icIEF separation and MS detection, also enables easily seamless icIEF method transfer from development to QC. This workflow underlined the outstanding importance of comprehensive separation techniques based on MS technology in the development of ADCs.

In-depth characterisation of mAbs & ADC charge variants with icIEF-HRAM MS

Learn more about researchers at Thermo Fisher Scientific how they use the CEInfinite icIEF-Preparative and online intact icIEF-MS for in depth characterization of mAbs, ADC charge variants and peptide mapping without extra interface. The CEInfinite simply your research by providing you consistency, repeatability and building platform methods . 

In-depth Characterisation of Fusion Proteins

Learn more about researchers at National Institutes for Food and Drug Control, who has developed a workflow integrating UHPLC-MS and CEInfinite icIEF-MS provided an innovative strategy for characterizing complex fusion proteins in the process of quality and manufacturing. 

New publication

MS study on SARS-CoV recombinant vaccine with comprehensive separation techniques to characterise complex heterogeneity. 

In this study, our CEInfinite icIEF enables the fractionation of all 18 peaks into 5 groups based on their pI values and collected fractions from each group were for offline SCX-MS characterization. Identified peaks from 1 to 4 using icIEF-MS online workflow are missed in the SCX-MS identification. A simple explanation for this is that our icIEF-MS has better sensitivity than SCX-MS due to icIEF sample preconcentration and a consistent nano-flow rate mobilisation.

The separation mechanism of icIEF and IEX is different from each other. Both are broadly used in biotherapeutics charge variant analysis, by combining them together, you can collect more complementary information.

Our CEInfinite icIEF-MS technology makes the protein heterogeneity characterization more straightforward, sensitive, high throughput and high data consistent.

The icIEF-MS online can be used for rapidly fingerprinting the complex heterogeneity of recombinant vaccine.

The icIEF fractionation is for simplifying the complexity of the protein samples, that allows you to carry out in-depth and more accurate characterization of collected fractions by HPLC-MS, and IEX-MS.

Read the publication here

                 Monoclonal antibody                                        Bispecific antibody                                        Fusion Proteins