Electrophoretic Automated Tissue Clearing

X-CLARITY™ Systems & Reagents for Tissue Clearing & 3D Imaging

The X-CLARITY™ Tissue Clearing System is an easy automated method for electrophoretic tissue clearing. The removal of lipids from tissues is done while preserving the structure of your sample. Do you clear a whole mouse brain at this moment in 2 days? Now you can do it in just 6 hours. 

The X-CLARITY™ Tissue Clearing System

Tissue clearing more efficient than ever before

How much time do you spend on preparing, sectioning, labeling, mounting, imaging, reconstructing, and analysing lots of ultra-thin slices? Imagine you never have to section again!

X-Clarity is the first complete electrophoretic tissue clearing system, a new polymerisation system, and ready-to-use reagents in one. Tissue clearing has never been faster or simpler. The method opens up a world of possibilities, from tracing neural circuitry to exploring the relationship between structure and function.

Main steps

1.    Hydrogel Infusion

A fixed tissue sample is incubated in a hydrogel monomer solution to allow hydrogel monomers to diffuse uniformly throughout the sample. 
The X-CLARITY™ Hydrogel Solution Kit is a ready-to-use acrylamide solution. 

2.    Hydrogel-Tissue Hybridisation

Once the hydrogel monomer solution has fully permeated the tissue sample, the monomers are polymerised to embed the tissue in a hydrogel matrix. The sample is heated, causing the thermal initiator to release free radicals and initiate radical polymerisation of the hydrogel monomers, covalently linking the biomolecules in the tissue sample to a sturdy hydrogel network, preserving molecular information and structural integrity. 

3.    Tissue Clearing

Once the tissue-hydrogel hybrid has been formed, lipids are broken up through electrophoresis in the presence of ionic detergents, leaving behind a stable and transparent tissue-hydrogel hybrid that is chemically accessible for molecular phenotyping. 
The X-CLARITY™ can clear a whole mouse brain in just 6 hours. 

4.    Labeling and Imaging

The tissue-hydrogel hybrid is placed in an RI matching solution prior to imaging to optimise transparency.

Labeling and imaging

DeepLabel™ Antibody Staining Kit for Cleared Tissues

Clearing methods allow light to penetrate samples for imaging, although the method used can affect tissue permeability to molecular probes. Conventional labeling protocols involve the slow diffusion of probes into thin sample sections, which translates to a time-consuming impracticality when applied to thicker samples. The slow progression of diffusion can also lead to uneven staining, with higher, nonspecific binding on the outside of a thicker sample. 

Mouse brain without DeepLabel

DeepLabel™ Antibody Staining Kit enhances antibody penetration into large clarified tissues for vibrant fluorescence 3D imaging. The new DeepLabel™ Antibody Staining Kit from overcomes these obstacles. Optimised for thick, cleared tissues, DeepLabel™ increases tissue permeability and enhances antigen-antibody binding so that antibody labeling of cleared tissues can happen rapidly and efficiently.

Mouse brain with DeepLabel


X-CLARITY™ Sistemas y reactivos para clarificación de tejidos e imágenes en 3D

 El X-CLARITY™ es un método automatizado sencillo para la clarificación electroforética de tejidos. La eliminación de lípidos de los tejidos se realiza preservando la estructura de su muestra. ¿Es posible clarificar todo el cerebro de un ratón en 2 días? Ahora puedes hacerlo en tan solo 6 horas.

¿Cuánto tiempo dedica a preparar, seccionar, etiquetar, montar, obtener imágenes, reconstruir y analizar muchos cortes ultrafinos? ¡Imagina que nunca más tendrás que seccionar!

X-Clarity es el primer sistema completo de clarificación electroforética de tejidos, un nuevo sistema de polimerización con reactivos ready-to-use. La clarificación de tejidos nunca ha sido tan rápida y sencilla. Este método abre un mundo de posibilidades, desde el rastreo de circuitos neuronales hasta la exploración de la relación entre estructura y función.

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