Sanger Sequencing

Name: Isogen Life Science B.V.
Address: Veldzigt 2a, De Meern, 3454 PW, NL
Telephone: +31 30 6880771

Save costs on your Sanger Sequencing kits - Affordable reactions

Are you in need of Sanger sequencing kits?

Are you planning to start your research with it?

Sanger Sequencing is a fast, cost-effective method of sequencing small targeted regions of the genome. It is widely used to test for known familial variants, for validation of results obtained through NGS, and for some single gene sequencing. We offer affordable and high quality kits for the whole workflow of your Sanger Sequencing experiments.

Explain us your workflow!

Sanger Sequencing: How the Genome Was Won

La secuenciación por el método de Sanger, utiliza la electroforesis capilar. Los pasos para el proceso de secuenciación de Sanger incluyen la fragmentación previa del ADN a secuenciar, seguido de la amplificación de estos fragmentos mediante la reacción en cadena de la polimerasa (PCR). A continuación, cada fragmento acabado en didesoxinucleótido está marcado fluorescentemente, de manera que cada didesoxinucleótido (ddATP, ddCTP, ddGTP, ddTTP) implica un color de fluoróforo distinto.

Los pasos importantes en la secuenciación de Sanger son los pasos de purificación y el uso de productos de alta calidad. Esto eliminará cualquier interferencia y aumentará la intensidad y el espaciado de los picos.

Desde Isogen Life Science ofrecemos kits para los distintos pasos del flujo de trabajo.

We offer kits from each of the workflow steps:

Template preparation
Cycle Sequencing Reaction Set-up
Regeneration of Capillary Array
Sequencing Clean-up and Capillary Electrophoresis (CE)

Have a look at some customer reviews!

Discover how our Sanger Sequencing kits can benefit you workflow

Template preparation

The purification step for PCR products, such as DNA polymerase, dNTPs or primers can be performed with use of magnetic beads. These beads are less time consuming and more inexpensive than the common used spin columns. They are adaptable to high throughput research, recover high amounts of DNA, form a uniform suspension and are suitable for a DNA sequencing workflow.

ADS PCR Cleaning Magnetic Beads: Our PCR cleaning beads enable you the following:

Adaptable with high-throughput template preparation
Compatible with DNA Sequencing workflow
High DNA recovery
Uniform bead suspension

For more information about it, have a look at the brochure here!

Also, we offer other kits and reagents like:

Taq DNA Polymerase
PCR Extraction kit
Nucleotides (dATP, dCTP, dGTP, dTTP and dNTP mix)
PCR green buffer

No need of changing protocol from your current kit.
Increased robustness for difficult samples (rich G and C samples).
Even peak heights and long read lengths.

Sequencing Reaction

In the Sanger DNA sequencing reaction, linear amplifications are labelled with one nucleotide difference with a ddNTP to terminate the chain. For this step we offer the SupreDye Cycle Sequencing Kit, which provides high performance, increase in peaks and longer read lengths. We also offer cycle sequence kits for high G-templates: SupreDye dGTP Sequencing Kit.

Achieve high performance and cost-effective quality results with our affordable cycle sequencing kits:

Sequence Clean-Up

Thereafter there is need for a sequence clean up to remove contaminants and unused ddNTPs. Different ways for this purification can be used. The ADS BD-XT Purification Kit is fast, reliable, has a simple work flow and does not need a follow up washing step. The ADS Sequence Reaction Cleaning Beads is another option for the purification, as the contaminants will bind to the beads, it can easily be removed. This method is ideal for DNA sequencing, requires a minimal amount of washing steps and is cost-effective.

Make sure contaminants and unincorporated ddNTP dyes from your sample are removed before sample loading. Discover our solutions:

Discover more about our affordable column-based purification kit
High quality sequencing with fewer washes and less dye-blobs.
Polymers for DNA sequencing (short and long reads) and fragment analysis.

Download the Brochure

Ask your questions or request a quote

Capillary Electrophoresis

After the polymerase chain reaction the sample can be loaded on capillary electrophoresis. Depending on the purification method, the sample can be directly loaded or needs resuspension. When the sample is purified with ethanol the sample is often resuspended with formamide. For the optimal signal intensity and stability TruPure formamide can be used. Another method is the use of polymers. The PwrPOP polymers are effective for separation of extension products. This is a cost-effective solution and creates even peak separation and long reads.

ADS Conformational Analysis Polymer
ADS Sequencing Running Buffer

Download the Brochure

Ask your questions or request a quote

Regeneration of the Capillary Array

After extensive usage of the capillary electrophoresis, there is a need for regeneration, which restores the performance of the capillary array. If this is not performed after approximately 500 runs the lifetime of the capillary array is reduced. The replacement of the capillary array is more expensive than the regular maintenance. The need for maintenance is recognisable on the uneven peak space and short reading lengths. For the regeneration we offer the ADS Capillary Regeneration Kit. It is easy to implement, has an on instrument protocol and a short hands-on time and there is no need for capillary disassembly.

Discover more about our Capillary array regeneration kit:

Achieve optimal sequencing performance (no polymer debris).
Save costs eliminating the need of replacing capillary.

Do you know that Isogen Life Science offers solutions from sample to result?

Have a look at our solutions for genomics, cell biology, diagnostics and proteins. We offer intruments, reagents, kits and consumables for your research!

Customer reviews

"The result looks really good. I am very happy with how the kit performed.” Core Director at a University.
“SupreDye v3.1 is a great, cost effective substitute to the original BDT v3.1. It gives equivalent (or better) results at a fraction of the cost, which helps keep our prices low for our academic customers.”—Core Lab Director
“We use your regeneration kit as part of our capillary maintenance. It really does help prolong the use of our capillaries.” Technical expert from a company
“Your ADS Capillary Regeneration Kit has been so good. I have capillaries still working great at 1000 runs! In the past I was changing capillaries at approximately 500 runs so the results are excellent. We have incorporated this regeneration into our regular maintenance and use it every 250 runs on each machine and this seems to really prolong the capillaries.”Scientist from a diagnostic company

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Why Isogen Life Science?

At Isogen Life Science, we believe that long term relationships with life science professionals and suppliers are fundamental to solve life science problems. Our curiosity drives us to continually stay involved and to dig deep in dialogue, with both clients and suppliers. It’s these intensive collaborations that enable us to develop tailored, innovative products that push life science’s boundaries. Partner up with us and enjoy our quick support for troubleshooting and questions!