Sanger Sequencing

Sanger Sequencing reagents kits

(Only available in Spain/Portugal)

Template preparation

When high grade reagents are used the elimination step for non-PCR products can be left out. The purification step for PCR products, such as DNA polymerase, dNTPs or primers can be removed with use of magnetic beads. These beads are less time consuming and more inexpensive than the common used spin column. They are adaptable to high throughput research, recovers high amount of DNA, form a uniform suspension and are suitable for a DNA sequencing workflow.

Products available for this step:

  • ADS PCR Cleaning Magnetic Beads
  • ADS Taq DNA Polymerase
  • ADS PCR and Purification kit
  • Nucleotides (dATP, dCTP, dGTP, dTTP)
    • dNTP mix
  • PCR green buffer

For another brand of PCR reagents click here

Magnetic Beads

Sequencing Reaction

In the sequencing reaction, linear amplifications are labelled with one nucleotide difference with a ddNTP to terminate the chain. For this step we offer the SupreDye Cycle Sequencing Kit, which provides high performance, increase in peaks and longer read lengths. We also offer cycle sequence kits for high G-templates: SupreDye dGTP Sequencing Kit.

Sequencing Reaction Cleaning Beads

Sequence Clean-Up

Thereafter there is need for a sequence clean up to remove contaminants and unused ddNTPs. Different ways for this purification can be used. The ADS BD-XT Purification Kit is fast, reliable, has a simple work flow and does not need a follow up washing step. The ADS Sequence Reaction Cleaning Beads is another option for the purification, as the contaminants will bind to the beads, it can easily be removed. This method is ideal for DNA sequencing, requires a minimal amount of washing steps and is cost-effective.

Download the ADS Sanger Sequencing Brochure

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Capillary Electrophoresis

After the polymerase chain reaction the sample can be loaded on capillary electrophoresis. Depending on the purification method, the sample can be directly loaded or needs resuspension. When the sample is purified with ethanol the sample is often resuspended with formamide. For the optimal signal intensity and stability TruPure formamide can be used. Another method is the use of polymers. The PwrPOP polymers are effective for separation of extension products. This is a cost-effective solution and creates even peak separation and long reads.

  • ADS Conformational Analysis Polymer
  • ADS Sequencing Running Buffer

Regeneration of the Capillary Array

After extensive usage of the capillary electrophoresis, there is a need for regeneration, which restores the performance of the capillary array. If this is not performed after approximately 500 runs the lifetime of the capillary array is reduced. The replacement of the capillary array is more expensive than the regular maintenance. The need for maintenance is recognisable on the uneven peak space and short reading lengths. For the regeneration we offer the ADS Capillary Regeneration Kit. It is easy to implement, has an on instrument protocol and a short hands-on time and there is no need for capillary disassembly.

Download the ADS Sanger Sequencing Brochure

Ask your question or request a quote

Regeneration Kit

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Contact information

+31 30 6880771
support@isogen-lifescience.com

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Veldzigt 2A
3454 PW De Meern

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