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NGS Sample preparation with the NanoDrop One Spectrophotometer
At present, qPCR is the most accurate quantification method available, but it is time-consuming and often requires vetting a standard operating procedure. As an alternative to qPCR, the NanoDrop One has been tested and shows promising results.
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A Dengue prediction test with huge potential
Dengue is a mosquito-borne viral infection, with almost half of the world’s population living in areas that are at risk of catching dengue.
"The NanoDrop is a very quick and straightforward way to determine these concentrations. With it, we can easily calibrate it, and we can then make use of standard reference materials to effectively set up our system. This means we can provide a control to the product development process, and this is better than any of the other methods that we used." Dr. Anthony Chua, StratifiCare.
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Small scale testing, large scale results - How one hospital's clinical testing challenges were alleviated with the help of our micro-volume spectrophotometer
In the high-stakes field of molecular diagnostics, accurate clinical testing contributes to a make-or-break decision between whether or not a patient receives a targeted therapy.
The NanoDrop™ One Microvolume UV-Vis Spectrophotometer is an instrument used in fundamental testing. It is a gold standard that enables molecular techniques providing key information on sample purity, as well as the quantity of DNA, RNA, proteins, oligos, and more.
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Quantifying Soy DNA extracts for downstream GMO detection
The controversy of grocery products containing genetically modified organisms (GMO) has heightened the demand for analytical testing in the food and beverage industry.
The determination of quantity and quality of template DNA, the NanoDrop™ Eight Microvolume UV-Vis Spectrophotometer calculates and identifies the concentration of contaminants from nucleic acid extractions.
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mRNA vaccines methodologies: Production value and spectrometer use
On the production of mRNA vaccines, it is needed the first steps of pathogen or virus isolation and nucleic acid extraction. In order to evaluate the purity and the concentration The NanoDrop™ Spectrophotometer is used before endpoint use of sequencing the genome to identify the antigen target.
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Breaking down boundaries using nicotine-producing plants to understand plant structure and cellulose synthesis
The use of the NanoDrop instrument helped on the regular basis measurement of DNA concentrations.
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Analysis of DNA melting through UV-Visible absorption spectroscopy
DNA melting, or denaturation, describes the process under which dsDNA disassociates into its single-stranded. The break of the hydrogel bonds needs the overcoming of an an energy activation barrier (e.g. High temepratures). The temperature which half of the dsDNA is denatured is called melting Temperature (Tm). Herein, the Tm for both small (40 base pairs
per strand) and large (~2,000 base pairs per strand; calf thymus) dsDNA sequences was determined and calculated with the NanoDrop Software.
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