Surface Plasmon Resonance (SPR) systems

Name: Isogen Life Science B.V.
Address: Veldzigt 2a, De Meern, 3454 PW, NL
Telephone: +31 30 6880771

SPR instruments

Surface plasmon resonance (SPR) can be used to measure binding events because it is very sensitive to changes in refractive index. SPR instrument are made up of an optical measurement system, a fluid handling system, and a sensor chip.

How does SPR work?
Digital, high-throughput, benchtop SPR System: Alto Instrument
Publication-quality binding kinetics and affinity data: OpenSPR
Intuitive Software for both instruments: Nicoysystem Software
Applications: protein-protein, protein-peptide and much more! Request them to us!
NEW: Publications!

How does SPR work?

The sensor chip is a gold film coated on a glass substrate that has been chemically modified to make it easier to immobilize one of the binding partners onto the surface of the sensor: the ligand. The molecule in solution is the analyte.

glass sensor chip below for SPR

The optical system consists of a light source and a detector. The light source illuminates the gold film, and the detector measures the unique optical spectrum produced by the SPR phenomenon. Most SPR instruments utilize a laser that shines through a prism beneath the sensor chip, generating a total-internal-reflection condition.

The reflected light will have a characteristic dip in intensity at a certain angle due to the plasmonic wave, which is measured with a detector. When a molecule binds to the surface of the sensor chip, the refractive index of the space in which the plasmonic wave is propagating through changes, and the reflectance angle will shift. The amount it shifts depends on how much mass of material has bound to the surface, and the shift can be measured in near real time.

Conventional vs Digital SPR? Get to know more about the difference here.

ALTO SPR SYSTEM

Alto is the world’s first SPR instrument to integrate digital microfluidics (DMF) with nanotechnology-based biosensors.

DMF technology enables all fluidics to be disposable, allowing for precise handling of 2µL sample volumes, crude sample compatibility, and zero fluidics maintenance. Alto’s compatibility with automation for both hardware and software provides sample-in/answer-out data, industry-leading throughput, and 24+ hours of unattended run time.

Every aspect of Alto is designed with user-friendliness and accessibility in mind-from the flexible 16 channel design to the intuitive data analysis platform.

Benefits:

Industry leading Data Quality: maximise data quality eliminating sample dispersion and evaporation.
Sample-Friendly: Get full kinetic analysis with only 2μL of crude or purified sample - 100X less than traditional SPR!
Spend 70% less time at the bench: High-Throughput Powered by Automation with no purification, dilutions, tagging, degrassing cleaning or manual assay design required.
User-friendly: label-free platform deisgned for biologics research

GxP Software expansion: The features in the Alto GxP software expansion are specifically designed to support compliance with the FDA’s 21 CFR Part 11 guidelines around electronic records.

alto digital spr

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OpenSPR

OpenSPR is the world’s onlybenchtop SPR instrument. It provides high quality, label-free interaction analysis for a fraction of the cost of existing solutions. Our unique nano-structured sensor surface uses localized SPR (LSPR) to deliver repeatable, highly sensitive kinetic data.

Benchtop
User-friendly
Real-time Data
Low-Maintenance
A broad range of comptible molecules: proteins, peptides, antibodies, antibody variants, nucleic acids, lipids, AAV, VLPs, small molecules, hormones etc.

Maximize your productivity with fully automated, 24/7 operation of our benchtop SPR with the OpenSPR-XT

Want to know more abou the system?

Get in touch with us!

Nicosystem Software

Alto’s comprehensive software, the Nicosystem, provides a one-stop centralized hub for acquisition and analysis of real-time binding data, all while offering an intuitive user interface, one-click analysis feature, and the flexibility of accessing your experiments from anywhere.

Intuitive experiment design: full suite of workflows for characterizing biomolecular interactions, including kinetics, screening, quantitation, and epitope binning/mapping.
Assay Applicaiton:
- Binding Kinetics: Elucidate the real-time kinetic binding profiles (ka, kd, KD) of up to 48 targets using multi-cycle or single-cycle kinetic analysis styles.
- Quantitation: Determine up to 40 unknown analyte concentrations per experiment.
- Screening: Screen up to 96 interactions in just a few hours and use Alto’s visualization tools to select the most relevant hits.
- Epitope binning: Process up to 256 interactions in a single cartridge with one-click analysis.

Automated analysis:

One-click analysis: Eliminate lengthy post-processing with Nicosystem’s one-click analysis. A diverse range of models and investigative tools are also available to accurately interpret your data and provide high-quality insights.
Flexible export: Export your data in a variety of file types, such as CSV files and images, in one click for continued data processing and reporting.

OpenSPR-XT system featured in Nature

Obi, J.O., Kihn, K.C., McQueen, L. et al. Structural dynamics of the dengue virus non-structural 5 (NS5) interactions with promoter stem-loop A (SLA). npj Viruses 3, 30 (2025). https://doi.org/10.1038/s44298-025-00112-1

University of Maryland researchers used our desktop OpenSPR-XT system to watch dengue-virus NS5 clamp onto its 5′-UTR promoter. The full enzyme bound at ≈ 14 nM kD, about 10× tighter than its separate domains, proving the domains need each other.

Read the publication

Why OpenSPR-XT made it easier?

Label-free, low-volume/maintenance: only 200 µL per injection—no tags, no complex system.
Hands-free: up to 24 h automated runs keep data flowing while you’re away.
Publication-ready kinetics in hours, dovetailing with HDX-MS & cryo-EM
Benchtop & budget-friendly, trusted by 500 + labs worldwide.

OpenSPR slots right into studies on viral polymerases (SARS-CoV-2, Zika, Flu), RNA–protein binding, or CRISPR guide interactions, delivering real-time on/off rates before lunch.

Doing similar work?

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Why Isogen Life Science?

At Isogen Life Science, we believe that long term relationships with life science professionals and suppliers are fundamental to solve life science problems. Our curiosity drives us to continually stay involved and to dig deep in dialogue, with both clients and suppliers. It’s these intensive collaborations that enable us to develop tailored, innovative products that push life science’s boundaries. Partner up with us and enjoy our quick support for troubleshooting and questions!