3d cell culture

Isogen LifescienceProductsInstruments3D Cell Culture: Functional Organoids Growth

3D cell culture in a standardized process:

The ClinoStat technology

Discover the new approach in 3D cell culture, for culturing your organoids and spheroids in application fields such as cancer research, drug screening, stem cell research, toxicology studies, personalized treatment, and many more.

The ClinoStat technology enables you to grow uniform spheroids and organoids without bias in your research. The research based on organoids and spheroids results in a closer approach to in vivo behaviour. Now, Organoid research is more accessible and easy with this standardized process. Do you want to discover how?

The Clinostat can work with different types of cell lines in 3D cell culture. It has been proven to be successful in stem cell differentiation, hepatocytes, bronchi cells, and brain and gut organoids/spheroids. At the moment, it is in use in different research labs using more cell lines.

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Are you working on 3D cell culture?

Discover more about our technology

The main difference between 2D and 3D cell culture is that when having 3D, cells do not stop developing and growing. Therefore, they grow, develop, and interact with one another, making research on 3D cells more similar in vivo. Additionally, the Clinostat offers less contamination because of the presence of the automatic UV-decontamination light. 

Moreover, it is a closed system. It has integrated a camera, eliminating the need of opening the incubator when it is not needed, eliminating fluctuance, and establishing a stable environment for your cells. The humidity and the CO2/O2 levels stay constant creating a higher quality of your organoid and spheroid.

Benefits of the 3D cell culture 

  • Gravity-free spinning environment for cell culture
  • Active gasses and nutrients diffusion with minimal shear forces. It enables the maintenance of active diffusion.
  • Organised long-life cell cultures: cell interactions from core to side prevent necrosis in the organoid centre.
  • Large Spheroids and Organoid formation
  • Long-time cell functionality
  • Unique airflow and filter system

Ideal for cancer research, drug screening, stem cell research, and many more applications. One of the main benefits related to application fields and regulations is that the use of 3D cell culture models limits the use of animals as research models.

Take CELLfies to track your 3D cell culture from remote!

The system goes together with 1 tablet, which enables the visualization via software of up to 50 different systems, which means the possibility of visualizing up to 300 different incubations.

Are you experimenting challenges in your 3D cell culture research? 

We would like to discuss with you how we can help you. We offer a DEMO possibility!

Contact us

Gravity-free movements 

The Clinostat uses gravity-free movements to your 3D cell culture to create less shear forces while maintaining active diffusion. This creates bigger and long-life organoid and spheroid cultures. The active diffusion enables living cells to receive nutrients and O2 from outside to the core of the culture, and metabolic waste to be diffused in the media. As a key outcome, the cells in your culture interact with one to another, mimicking organism behaviour.

active diffusion that enables the arrival of nutrients from side to the core of the organoid.

 

 

 

 

 

 

 

                                     Watch the video

The ClinoStar can introduce 6 individual incubators to grow your cells. The system has a small footprint. It can be stackable up to 3 units, fitting in any lab. Moreover, CO2 level and temperature can be adjusted to optimise your cell growth. Also, you can control the rotation speed per incubator, enabling the culture of different organoids with different conditions.

Your 3D cell culture will be performed inside the ClinoReactor, which is composed of three main parts: the outside consists of hydro balls. 

They promote humidification and gas exchange. The middle chamber is a 10mL growth chamber in a shape of a Petri dish where you incorporate your cells to culture. Finally, the bioreactor contains two openings to easily exchange de medium when needed.

 

Are you working on 3D cell culture and you are based in the NL, BE, LX, SP, PT or the UK? Do you want to try the system in your workflow and lab space? Contact us for a demo opportunity!

The most recent Applications 

Do you want to be updated with the newest publications in 3D cell culture? You can have a look below or contact us!

3D Cell Culture and Epigenetics

This Application Note reports the use of ClinoReactors for culturing 3D liver spheroids to model chromatin dynamics and the response of epigenetics inhibitors.

Author: PhD Stephanie Stransky (Albert Einstein College of Medicine in NY)

 Read the Publication 

 

Liver disease Research

This Application Note reports the use of ClinoReactors for generating a model for early steatosis in NAFLD. The model is based on the 3D culture of human hepatic carcinoma cells which are exposed for seven days to physiological levels of fatty acids. 

Author: Helle Sedighi Frandsen, et al. (Department of Biochemistry and Molecular Biology, Universiry of Southern Denmark).

Read the publication 

Enhanced model for Triple-Negative Breast Cancer Therapeutics

Breast cancer is the most prevalent cancer affecting women and the second leading cause of cancer deaths among women. Triple-negative breast cancer (TNBC) is the most aggressive form with the lowest survival rate.

Read the publication

Bovine Blostocyst structure from stem cells

The Application Note presents the development of an efficient method to generate bovine blastocyst-like structures from stem cells to future study blastocyst formation and implantation.

Author: Carlos A. Pinzón-Arteaga et al. Cold Spring Harbor Laboratory, BioRxiv. https://doi.org/10.1101/2022.12.20.521301 

Read the publication

 

Respiratory viruses infection: Omicron BA.5 and the brain

The Application Note presents the study of the variant Omicron BA.5 infectious and side effects. A mice brain organoids was developed succesfully with the use of the ClinoStar for the specific study. 

Author: Romal Stewart et al. Cold Spring Harbor Laboratory, BioRxiv. https://doi.org/10.1101/2022.12.22.521696

Read the publication

The newest White paper: How to generate human brain organoids 

Discover how to grow and expand neural organoids in 3D. The white papern mentioned below addresses the challenges of growing human brain organoids and covers how rotational 3D can benefit the growth of neural organoids.

Get to know the protocol from researchers at QIMR in Australia and how they have grown neural organoids in the ClinoStar.

Author: Senior Application Scientist Reeham W. Motaher, PhD (USA)

 

 

 

 

Read the publication

 

Moreover, the technology for growing successfully you 3D cell culture has been used in culturing all the lines listed below:

  • Cryopreserved human primary hepatocytes
  • Immortal hepatoma cell line HepG2/C3A and Huh-7
  • Immortalised hepatocytes (SV40 transformed): Fa2N-4
  • Human hepatic progenitor cell line: HepaRG
  • Human microvascular endothelial cell line: HMEC-1
  • Human monocyte Kupffer-cell-like cell line : THP-1
  • Human Colorectal adenocarcinoma cell line: LS180
  • Human adenocarcinoma cell line: HeLa
  • Human small cell lung cancer: NCI-H69 and sub clones
  • Rat insulinoma cell line: INS-1 and INS-1E
  • Rat islet cell line: RIN-m5F
  • Mouse pre-adipose embryo fibroblast cell line: 3T3-L1
  • Freshly isolated monkey (baboon) and rat islets of Langerhans
  • Freshly isolated human colon cancer biopsies

Customer Reviews

Customer review

Cellular Mechanism of Cancer Cells

"With the new ClinoStar, we are able to maintain long-term 3D cell cultures that are used not only for tissue engineering approaches, but also to study the complex processes that allow a cancer cell to survive as a metastasis. This is critical for exploring new therapies to destroy cancer cells with metastatic properties. The ClinoStar will help to understand the cellular mechanisms that lead to metastasis in vitro and to develop anticancer countermeasures in vivo."

Dr. rer. nat. Marcus Krüger Medizinische Fakultät Universitätsklinikum Magdeburg

Customer review

Mutation Analysis of the Pacreas with Organoids

The Pedersen team investigates acid-base- and metabolite homeostasis in cancer development, metabolism and invasiveness. We combine state-of-the-art 3D cell culture with live imaging, mouse models, molecular- and computational biology.

Our ongoing projects focus on the interplay between driver mutations and adaptation to acidic microenvironment in pancreatic ductal adenocarcinoma (PDAC), using spheroids and organoids as model systems. Organoids derived from murine cancer models or patient tumours are valuable tools for precision medicine and drug development, however their standard culture method is expensive, time-consuming and low-throughput. We use the CelVivo ClinoStar system to grow spheroids and organoids quickly and in large quantities from small starting materials, mimicking in vivo-like intercellular communication, ultrastructure and metabolism. We also appreciate the potential that the ClinoStar system has for limiting the use of animal models in our research.

Prof. Stine Falsing Pedersen Copenhagen University

Customer review

University of Antwerp

“Expanding organoids for drug screening has always been time consuming and labour intensive. We are astonished how much faster organoids in the ClinoStar and by the ease of use for maintaining long-term cultures”. 

Christophe Deben Antwerp University

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