The Clinostat uses gravity-free movements to create less shear forces, while maintaining active diffusion. This creates bigger and long-life organoid and spheroid cultures. The active diffusion allows for living cells in the core of the culture as on the outside of the culture. This way the organoid culture can interact with one other, as also occurs in organisms.
The vertical bioreactor has 6 individual incubators to grow your cells. CO2 level and temperature can be adjusted to optimise your cell growth. Even as the rotation speed, which can be adjusted individually per incubator. With the built-in fan, a uniform environment is created.
The system has a small footprint and is stackable up to 3 units, and therefore fits in any lab.
In the clinostat, you have room for 6 disposable bioreactors. The outside of these reactors consists of hydro balls, which promotes the humidification and gas exchange. In the middle there is a 10 mL growth chamber (petri-dish) for your cell cultures. The circular reactors can stand vertical with an opening at the top for easy medium exchange. The front port allows for easy sampling of the organoids and spheroids.
"With the new ClinoStar, we are able to maintain long-term 3D cell cultures that are used not only for tissue engineering approaches, but also to study the complex processes that allow a cancer cell to survive as a metastasis. This is critical for exploring new therapies to destroy cancer cells with metastatic properties. The ClinoStar will help to understand the cellular mechanisms that lead to metastasis in vitro and to develop anticancer countermeasures in vivo."
The Pedersen team investigates acid-base- and metabolite homeostasis in cancer development, metabolism and invasiveness. We combine state-of-the-art 3D cell culture with live imaging, mouse models, molecular- and computational biology.
Our ongoing projects focus on the interplay between driver mutations and adaptation to acidic microenvironment in pancreatic ductal adenocarcinoma (PDAC), using spheroids and organoids as model systems. Organoids derived from murine cancer models or patient tumours are valuable tools for precision medicine and drug development, however their standard culture method is expensive, time-consuming and low-throughput. We use the CelVivo ClinoStar system to grow spheroids and organoids quickly and in large quantities from small starting materials, mimicking in vivo-like intercellular communication, ultrastructure and metabolism. We also appreciate the potential that the ClinoStar system has for limiting the use of animal models in our research.
This Application Note reports the use of ClinoReactors for culturing 3D liver spheroids to model chromatin dynamics and the response of epigenetics inhibitors
Author: PhD Stephanie Stransky (Albert Einstein College of Medicine in NY)