3D PETG Cell Culture Scaffold

Name: Isogen Life Science B.V.
Address: Veldzigt 2a, De Meern, 3454 PW, NL
Telephone: +31 30 6880771

Accelerating the 3D approach to cell culture

3D cell culture creates a 3D architecture for cell growth, offering a closer physiological environment. The growth of cells on 3D scaffolds promotes accurate cell-to-cell and cell-to-extracellular environment interactions with variable access to oxygen, nutrients and metabolites.

Mammalian cells in this environment mimick their tissuein vivo, and therefore, they exhibit more relevant gene expression, splicing topology and biomarkersin vitro. With this cell performence, the reliability in terms of drug and toxicity modelling
in the lab increases dramatically.

Scaffolds and our 3D PETG scaffold technology
Cellular attachment and proliferation

Learn more about it!

Have a look at the poster

Scaffolds for a better 3D Culture Environment

Why are scaffolds important? They are porous, biocompatible and biodegradable materials that provide the optimal microenvironment for cell growth. Therefore, mechanical, physical and biochemical support is provided to cells enabling them to grow and function: aggregate, proliferate, and migrate.

3D PETG Scaffold

Our technology is produced thanks to a dedicated scaffold shotware which enables nutrient and oxygen gradients to maximise cell growth. This enables to develop better models in the lab for a better drug and model understanding in disease conditions.

The introduction of a discrete oxygen gradient across the interface of our 3D PETG scaffold, promotes cell proliferation from the centre to the periphery, resulting in a much more balanced system of cells on the scaffold, with confluency patterns like in vivotissue.

Our scaffold enhances cell capture and adhesion, as well as encouraging the growth of balanced, confluent cell systems. Have a look below for more details!

The produced scaffolds have been validated as successfull on 3D cell culture growth for organoids and spheroids in collaboration with TCH laboratory and Advanced Tissue Culture Suit.

Learn more about Copner Biotech scaffolds initiative

Watch a video!

Cellular attachment and proliferation

Our 3D PETG scaffold is considered a noncytotoxic material. It prooved to be safe for use in cell culture, showing no
negative effects on cell growth or function, and making your transition from 2D to 3Dcell culture easier.

User friendly: rigid and no degradable scaffolds
Long term culture of cells
Significant expansion of cultures over a short time

Cellular Attachment
Copner Biotech’s 3D PETG scaffolds efficiently mediates mammalian cell attachment, whilst using low cell seeding concentrations.

The different cells that have been used up to date are the ones listed below, but no limited.

L929 Fibroblasts
Keratinocytes
HeLa cells
MCF-7 cells
A549 cells
iPSCs.

SEM images of dermal spheroids cultured on 3D PETG Scaffold.

Fibroblasts and keratinocytes were cultured for 7 days, after seeding at 100K concentration on day 1. Spheroids were easily
harvested from scaffolds by aspirating with pipette and showed reduced damage compared to methods of harvesting from hydrogel syste

Feel free to look at the brochure and protocols!

Why Isogen Life Science?

At Isogen Life Science, we believe that long term relationships with life science professionals and suppliers are fundamental to solve life science problems. Our curiosity drives us to continually stay involved and to dig deep in dialogue, with both clients and suppliers. It’s these intensive collaborations that enable us to develop tailored, innovative products that push life science’s boundaries. Partner up with us and enjoy our quick support for troubleshooting and questions!