3D PETG Cell Culture Scaffold

Accelerating the 3D approach to cell culture

3D cell culture creates a 3D architecture for cell growth, offering a closer physiological environment. The growth of cells on 3D scaffolds promotes accurate cell-to-cell and cell-to-extracellular environment interactions with variable access to oxygen, nutrients and metabolites.







Mammalian cells in this environment mimick their tissuein vivo, and therefore, they exhibit more relevant gene expression, splicing topology and biomarkersin vitro. With this cell performence, the reliability in terms of drug and toxicity modelling
in the lab increases dramatically. 



Scaffolds for a better 3D Culture Environment 

Why are scaffolds important? They are porous, biocompatible and biodegradable materials that provide the optimal microenvironment for cell growth. Therefore, mechanical, physical and biochemical support is provided to cells enabling them to grow and function: aggregate, proliferate, and migrate. 

3D PETG Scaffold

Our technology is produced thanks to a dedicated scaffold shotware which enables nutrient and oxygen gradients to maximise cell growth. This enables to develop better models in the lab for a better drug and model understanding in disease conditions. 

The introduction of a discrete oxygen gradient across the interface of our 3D PETG scaffold, promotes cell proliferation from the centre to the periphery, resulting in a much more balanced system of cells on the scaffold, with confluency patterns like in vivotissue.

Our scaffold enhances cell capture and adhesion, as well as encouraging the growth of balanced, confluent cell systems. Have a look below for more details!

The produced scaffolds have been validated as successfull on 3D cell culture growth for organoids and spheroids in collaboration with TCH laboratory and Advanced Tissue Culture Suit.

Learn more about Copner Biotech scaffolds initiative

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Cellular attachment and proliferation

Our 3D PETG scaffold is considered a noncytotoxic material. It prooved to be safe for use in cell culture, showing no
negative effects on cell growth or function, and making your transition from 2D to 3Dcell culture easier.

  • User friendly: rigid and no degradable scaffolds
  • Long term culture of cells 
  • Significant expansion of cultures over a short time

Cellular Attachment
Copner Biotech’s 3D PETG scaffolds efficiently mediates mammalian cell attachment, whilst using low cell seeding concentrations.

The different cells that have been used up to date are the ones listed below, but no limited. 

  • L929 Fibroblasts
  • Keratinocytes
  • HeLa cells
  • MCF-7 cells
  • A549 cells
  • iPSCs.

SEM images of dermal spheroids cultured on 3D PETG Scaffold.

Fibroblasts and keratinocytes were cultured for 7 days, after seeding at 100K concentration on day 1. Spheroids were easily
harvested from scaffolds by aspirating with pipette and showed reduced damage compared to methods of harvesting from hydrogel syste

Customer reviews

Customer review

In vitro chronic wound infection model

We have been using Copner Biotech’s bio-printer todevelop and modify our in vitro chronic wound infection model. The support and guidance we received following the installation of the bio-printer has been both excellent and invaluable, and it’s great that were still are able to discuss our bio-printing project and needs as the work progresses.

The bio-printer doesn’t need a lot of bench-space, and is easy to set up and use; I’m looking forward to seeing where this technology continues to take the groups research.

Customer review

Robust scaffolds for stem cells growth

The scaffolds are incredibly easy to use. The preparation protocol is simple to follow and quick to do. My stem cells attached, proliferated and migrated onto the scaffolds with ease, with no adaptation needed. The scaffolds are robust and easy to handle.

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