ATP Kits

All living cells contain ATP (adenosine triphosphate) and when the cell dies ATP is degraded by intracellular enzymes to ADP and finally AMP. The intracellular ATP concentration is similar in different cell types. Bacterial cells contain 1-2 attomoles per cell while animal and plant cells contain 100 to 10 000 times as much ATP. 

Extracellular ATP may be degraded by adding ATP consuming enzymes or by physical separation. Determination of intracellular ATP can then be used to estimate biomass. If the amount of ATP per cell is known the cell number may also be estimated. 

In sample which likely contains urine, extracellular ATP, ATP in somatic cells and ATP in bacterial cells all three ATP pools can be determined.

ATP kits

Determination of bacterial ATP in urine is used to determine bacteria in the diagnosis of urinary tract infection.
 All ATP assays shall be calibrated by measuring the light before and after adding a known amount of ATP standard at the end of each test. This calibration obviates analytical interference from all sorts of variation of reagents and sample matrices and provides a result expressed in moles rather relative light units.

ATP kit SL

The ATP kit SL is made for monitoring ATP in the range of  10-12 to 10-6 mol/L. The enzymatic formation or degradation of ATP and cellular release of ATP will be monitored by measuring the light intensity. The ATP SL kit is good for 200-1000 assays, depending on the assay volume. The reagents kit contains ATP reagents with luciferase, Diluent, ATP standard and Tris-EDTA buffer. 

Applications

  • Assays of enzymes and metabolites participating in ATP converting reactions
  • Monitoring of oxidative phosphorylation and photophosphorylation
  • ATP release during cell lysis.
  • ATP release during platelet aggregation
ATP kit

Benefits of the ATP kit SL

  • Fast assay - Results within minutes
  • Stable light - Half-life >1h
  • Detection limit - 10-15 mol or 10-12 mol/L ATP
  • Reliable - Liquid ATP standard
  • Flexible - microplates and cuvettes can be used
ATP hygiene kit

ATP Hygiene kit HS

The ATP Hygiene kit is made for detection of ATP on the surface of liquids. The kit contains reagents for 25 assays. The reagents kit contains ATP reagent with luciferase, diluent, extractant and an ATP standard. 

Applications

  • ATP in liquids or on surfaces is an indicator of
    biological contamination. 
  • Food industry (HACCP)
  • Hospitals
  • Clean room facilities
  • Liquid samples that can be assayed include:
    • Drinking water
    • Rinsing water (e. g. from CIP plants)
    • Process water (e. g. from cooling water towers)
    • Water from public baths.

 

Benefits of the ATP Hygiene kit

  • Fast assays - Results within minutes
  • Highly sensitive - 10-17mol ATP (~5 bacteria)
  • Reliable - Liquid ATP standard
  • User-friendly - Dropper bottles, no pipetting

Microbial ATP Kit

The microbial ATP kit is made for the determination of intracellular ATP in microbial cells, after the elimination of non-microbial ATP. The kit is made for 150-375 assays. The ATP reagents kit contains ATP reagent, with luciferase, diluent, extractant, ATP standard, ATP eliminating reagents and Cell Lysing Reagent.

Applications

Due to the high sensitivity of the kit, low ATP levels can be detected.

  • Bacteriological control of liquids (e.g. drinking and process water, beverages and beer)
  • Pharmaceuticals and cosmetics
  • Cell adhesion to surfaces
  • Quality assurance of laundry
  • Bacterial growth
  • Antibiotic effects on bacteria
Microbial ATP kit

Benefits of the Microbial ATP kit

  • Fast assays - Results within minutes
  • Highly sensitive - 10-17mol ATP (~5 bacteria)
  • Reliable - Liquid ATP standard
  • Flexible - Microplates and cuvettes methods
Biomass ATP kit

Biomass ATP Kit

The Biomass ATP kit is ideal for determination of total ATP (extra- and intracellular) in samples with living cells. The ATP kit is perfect for determination of very low ATP levels. The ATP kit can be used in 150-375 assays. The reagents kit contains ATP reagents with luciferase, diluent, extractant and an ATP standard.

Applications

  • Bacteriological control of liquids (e.g. drinking or process water, beverages and beer)
  • Pharmaceuticals and cosmetics
  • Cell adhesion to surfaces
  • Quality assurance of laundry
  • Bacterial growth
  • Antibiotic effects on bacteria

Benefits of the Biomass ATP kit

  • Fast assays - Results within minutes
  • Highly sensitive - 10-17mol ATP (~5 bacteria)
  • Reliable - Liquid ATP standard
  • Flexible - Microplates and cuvettes methods
  • Efficient extractant - Animal and microbial cells

Intracellular ATP kit

This kit is ideal for determination of intracellular ATP in living cells, as the extracellular ATP will be enzymatic degraded. Subsequently, intracellular ATP is released from the cell by the extractant, which also inactivates the ATP degrading enzyme. With a luminometer the light intensity will be measured before and after addition of the ATP standard. The intracellular kit contains ATP reagents for 150-375 assays. The reagents kit contains ATP reagent containing luciferase and luciferin, diluent, extractant, ATP standard and ATP eliminating reagent. The sensitivity of this kit allows determination of low ATP levels in a variety of circumstances, provided it is not important to degrade ATP in somatic cells.

Benefits of the Intracellular ATP kit

  • Fast assay - result within minutes
  • Highly sensitive - 10-17 mol ATP (~5bacteria)
  • Reliable - Liquid ATP standard
  • Flexible - use of microplate or cuvettes

Applications:

    • Bacteriological control of liquids (e.g. drinking and process water, beverages and beer)
    • Pharmaceuticals and cosmetics
    • Cell adhesion to surface
    • Quality assurance of laundry
    • Bacterial growth
    • Antibiotic effects of bacteria

Cellular ATP kits

The cellular ATP kit HTS is ideal for enumeration of viable cells by quantification of cellular ATP. Quick analysis of proliferation and cytotoxicity of mammalian cells. This high throughput screening method  eliminates the need for traditional methods as tritiated thymide incorporation and tetrazolium salt reduction. The cellular ATP kit can be used for 1200 assays. The cell kit contains ATP reagent with luciferase, Lysing diluent and ATP standard. 

Applications

  • Drug discovery
  • Toxicity testing
  • Hormone effects
  • Tumour chemosensitivity testing
  • Cell proliferation
Cellular ATP kit

Benefits of the Cellular ATP kit

  • Fast assays - Results within minutes
  • Sensitive - 10-15 mol ATP (single cell detection)
  • User-friendly - Just mix and measure
  • Reliable - Stable, Ready-to-use ATP standard
  • Cost efficient - No standard curve required
Luciferase Assay kit

Luciferase Assay kit

The luciferase kit optimises assays of luciferase activity in vitro in reporter gene studies. The kit is available for cuvette and high throughput screening. The kit contains ATP substrate, Luciferin substrate and Tris-EDTA buffer. 

Benefits of the luciferase assay kit

  • Firefly luciferase is not present in normal cells
  • Sensitive (10-19mol luciferase and easy to perform
  • Cost-efficient - High throughput screening kits are available
  • Flexible - microplates and cuvette methods are possible

Kinase Reaction Rate kit 

The Kinase reaction rate kit is specially developed for easy and reliable identification of protein kinase inhibitor candidates. This identification is performed using a large compound library. The Kinase RR kit uses direct bioluminescent measurements of ATP degradation rate. This distinguishes hits from non-hits. Besides that, the standard curve is linear instead of sigmoidal. The Kinase RR kit can be adapted in high throughput screening studies. No radioactive materials, antibodies or other conjugates are needed for these assays.

The Kinase RR kit contains a buffer, ATP standard, ATP reagent, diluent and a blank.

Applications

  • Assays of any type of protein kinase
  • Substrate optimisation
  • HTS assays of compound libraries
  • IC50 determination

Benefits of the Kinase RR kit

  • Identifies kinase inhibitors without interference from luciferase inhibition
  • Wide linear range with Z' values up to 0.96
  • Low cost, one-step procedure
  • Applicable for all type of kinases

Category

Subcategory

Type

Code
Description

We are happy to help you.

You can contact us by filling in the form below. We will try to answer your question within one working day.

Do you want to know what we do with your data from this form? Then read our privacy statement.

Contact information

+31 30 6880771
support@isogen-lifescience.com

Our office

Veldzigt 2A
3454 PW De Meern

ISOgen office