ATP Kits

All living cells contain ATP (adenosine triphosphate) and when the cell dies ATP is degraded by intracellular enzymes to ADP and finally AMP. The intracellular ATP concentration is similar in different cell types. Bacterial cells contain 1-2 attomoles per cell while animal and plant cells contain 100 to 10 000 times as much ATP. 

Extracellular ATP may be degraded by adding ATP consuming enzymes or by physical separation. Determination of intracellular ATP can then be used to estimate biomass. If the amount of ATP per cell is known the cell number may also be estimated. 

In sample which likely contains urine, extracellular ATP, ATP in somatic cells and ATP in bacterial cells all three ATP pools can be determined.

Microbial ATP Kit

The microbial ATP kit is made for the determination of intracellular ATP in microbial cells, after the elimination of non-microbial ATP. The kit is made for 150-375 assays. The ATP reagents kit contains ATP reagent, with luciferase, diluent, extractant, ATP standard, ATP eliminating reagents and Cell Lysing Reagent.

Applications

Due to the high sensitivity of the kit, low ATP levels can be detected.

• Bacteriological control of liquids (e.g. drinking and process water, beverages and beer)
• Pharmaceuticals and cosmetics
• Cell adhesion to surfaces
• Quality assurance of laundry
• Bacterial growth
• Antibiotic effects on bacteria

Intracellular ATP kit

This kit is ideal for determination of intracellular ATP in living cells, as the extracellular ATP will be enzymatic degraded. Subsequently, intracellular ATP is released from the cell by the extractant, which also inactivates the ATP degrading enzyme. With a luminometer the light intensity will be measured before and after addition of the ATP standard. The intracellular kit contains ATP reagents for 150-375 assays. The reagents kit contains ATP reagent containing luciferase and luciferin, diluent, extractant, ATP standard and ATP eliminating reagent. The sensitivity of this kit allows determination of low ATP levels in a variety of circumstances, provided it is not important to degrade ATP in somatic cells.

Benefits of the Intracellular ATP kit

• Fast assay - result within minutes
• Highly sensitive - 10^-17 mol ATP (~5bacteria)
• Reliable - Liquid ATP standard
• Flexible - use of microplate or cuvettes

Applications:

• • Bacteriological control of liquids (e.g. drinking and process water, beverages and beer)
  • Pharmaceuticals and cosmetics
  • Cell adhesion to surface
  • Quality assurance of laundry
  • Bacterial growth
  • Antibiotic effects of bacteria

Kinase Reaction Rate kit 

The Kinase reaction rate kit is specially developed for easy and reliable identification of protein kinase inhibitor candidates. This identification is performed using a large compound library. The Kinase RR kit uses direct bioluminescent measurements of ATP degradation rate. This distinguishes hits from non-hits. Besides that, the standard curve is linear instead of sigmoidal. The Kinase RR kit can be adapted in high throughput screening studies. No radioactive materials, antibodies or other conjugates are needed for these assays.

The Kinase RR kit contains a buffer, ATP standard, ATP reagent, diluent and a blank.

Applications

• Assays of any type of protein kinase
• Substrate optimisation
• HTS assays of compound libraries
• IC50 determination

Benefits of the Kinase RR kit

• Identifies kinase inhibitors without interference from luciferase inhibition
• Wide linear range with Z' values up to 0.96
• Low cost, one-step procedure
• Applicable for all type of kinases