Labelling Chemistry

Labelling Chemistry

When you are looking for the best reagents and kits regarding labelling chemistry, such as reagents for Western blotting, antibody labelling or even HTRF (Homogenous Time Resolved Fluorescence) technology, you are at the right place!
We offer you products regarding all previously mentioned techniques produced by Cyanagen, Advansta, Intas and Bio-Nobile. Leading companies in the field of reagents for molecular biology techniques. Their products are based on patented technologies in which outstanding performance in sensitivity and stability can be expected. 

Scroll down or click on the topics below to read more. 

DNA / RNA Detection

 

HDGreen™ Plus Dye by Intas

A non-toxic fluorescent dye for the detection of DNA / RNA in agarose gels. When Intra HDGreen™ Plus is excited by UV or blue LED light, the stained DNA bands are optimal visible in gel documentation systems.

The Intas HDGreen™ Plus DNA Dye is ahead of other competitor products, as the dye achieved significantly better results in tests than the already very successful Intas HDGreen™ DNA Dye.

Mutagenicity tests are performed by an independent laboratory, and classified Intas HDGreen™ Plus as non-mutagenic.

Download the HDGreen Plus Safe DNA Dye report

Ask your questions or request a quote.

 

Intas HDGreen DNA Stain

Green Stain – fluorescent stain for nucleic acid detection in gels

Green Stain is a 1000X (in DMSO) fluorescent dye which is easy-to-use for nucleic acid detection in electrophoretic gels. It can be used in pre-electrophoresis gel staining, sample pre-staining as well as in post-electrophoresis gel staining. 

Features

  • Easy-to-use
  • Highly sensitive
  • Very safe

Ruby Red Stain – fluorescent stain for protein detection in gels

Ruby Red Stain is a ready-to-use kit for quick and sensitive protein staining in polyacrylamide gels. It makes it possible to nicely visualize and quantify protein with the same sensitivity as silver staining without its drawbacks.  The kit also includes fixing and destaining solutions. 

Features

  • High purity dye
  • Strong and reproducible signal
  • Fast staining protocol

Ruby Red gel staining on a 1D gel. A two-fold dilution series of Precision Plus Protein™ was used. 
Unstained Bio-Rad marker, separated on a Bio-Rad Mini PROTEAN® TGX™ 12%. 
Lane 9 is about 0.4 ng. 

For more information download our brochure.

Ask your questions or request a quote.

Western blotting: Cyanagen and Advansta

 

WESTAR HYPERNOVA by Cyanagen

WESTAR is a product line for enhanced chemiluminescence (ECL) substrates for Western blotting applications. ECL is commonly used for Western blotting detection since it is an easy method with great sensitivity. The new WESTAR HYPERNOVA is the brightest HRP (Horseradish Peroxidase) substrate available. The signal intensity is extremely high and has a stable light output which makes it suitable for detecting trace protein levels. A low background results in an extremely high signal-to-noise ratio. 

Features

  • Ultrasensitive -> Detection of low protein levels
  • Short exposure time
  • High signal-to-noise ratio
  • Less antibodies and (precious) samples are needed -> money-saving

A.

B. 

High sensitivity of WESTAR HYPERNOVA compared to other substrates (GEHC-Amersham ECL Select & SuperSignal™ West Femto-Thermo Scientific™)
A)    Detection of purified IκBα with WESTAR HYPERNOVA and two other substrates (1st Ab: Rabbit anti-IκBα, 1:1000, followed by 2nd Ab: Goat anti-rabbit, 1:500.000). A 1.5-fold dilution series of purified IκBα (from 667 pg to 5 pg) was imaged simultaneously with both substrates for 10 seconds with ImageQuant™ LAS 4000 (GE Healthcare).
B)    Signal-to-noise ratio (S/N) analysis for all substrates. The enlarged inset shows the increased sensitivity of WESTAR SUPERNOVA substrate. LOD = 5 pg compared to LOD = 39 pg, SuperSignal™ West Femto (LOD = limit of detection). 

Download the Brochure

Other available ECL substrate kits are:

  • WESTAR ECL-Sun
  • WESTAR Nova 2011
  • WESTAR EtaC
  • WESTSTAR EtaC Ultra
  • WESTAR Supernova

Download the Brochure

Ask your questions or request a quote.

Advansta Western blotting substrates


WesternBright MCF
Advansta’s WesternBright multi-colour fluorescent (MCF) Western blotting kit enables visualisation of two proteins at once, and thereby increasing the quality and quantity of information that can be gained from a single blot. Imaging a control protein alongside a protein of interest is possible with this kit. Just like imaging phosphorylated and unphosphorylated isoforms of a protein simultaneously. The fluorescent dyes provided with WesternBright MCF outperform Cy™ dyes and allow detection of low pg of protein. The WesternBright protocol saves time and money since there is no need for disposable film, and the blot can be imaged immediately, without drying. 

Detection of phosphorylated and unphosphorylated isoforms of a protein simultaneously.

 EGFR and phospho-EGFR can be imaged simultaneously using WesternBright MCF Western blotting kit. Chemiluminescence detection of the same experiment would require stripping and reprobing of the blot which can result into more errors and thereby influencing the outcome. A431 cell lysates (lane1) and A431 cells treated with EGF (lane 2) were blotted. EGFR is detected in green (b) and phospho-EGFR in red (c). An overlay picture is seen in (a). Molecular weight marker in lane 3. 

WesternBright ECL spray

WesternBright ECL spray is optimized for chemiluminescent Western blots imaged using film. The same components as Advansta’s WesternBright ECL link to webshop, but put together in one easy spray which makes measuring or mixing unnecessary. Simply spray onto the blot and you are ready to image. 
WesternBright ECL is a very strong substrate which allows you to use up to 10x less antibody compared to other substrates. Due to the fewer steps needed, less pipettes, tips and tubes are used resulting in additional savings and reduced waste. 

Features:

  • Easy to use
  • Use 10x less antibody
  • 4x more sensitive compared to others
  • Signal lasts more than 6 hours

Download the Brochure

Next to this, we offer other Western blotting products as well, such as:

  • Blot film
  • BCA kits
  • Loading control antibodies
  • Protein markers
  • Stripping buffers
  • And more

Antibody labelling technologies

Cyanagen: 

  • Secondary antibodies (link naar STAR FLUOR Secondary Antibodies by Cyanagen)
  • ELISA (link naar ELISTAR HYPERNOVA for ELISA)

BioNobile: 

  • HTRF (Homogenous Time Resolved Fluorescence) (link naar QuickAllAssay by BioNobile)

STAR FLUOR Secondary Antibodies by Cyanagen

STAR FLUOR secondary antibodies are secondary antibodies of high quality. They are designed for use in fluorescent immunoassays such as flow cytometry, cell imaging and fluorescent Western blotting. 
Detection of low protein amounts is possible due to the superior brightness of the antibodies where high signal-to-noise ratio is guaranteed. 

Features

  • Superior brightness for high sensitive detection
  • Low background signal, so high signal-to-noise ratio
  • Stable signal makes repetitive and long exposure possible
  • Low cross-reactivity

Human Transferrin visualized by fluorescent Western blotting using Rabbit anti-human transferrin primary antibody and Goat anti-Rabbit Star Fluor550 (in red), Goat anti-Rabbit Star Fluor645 (in green). Proteins are imaged with ImageQUANT™ LAS 4000 ( GE Healthcare). 

Download the Brochure

Ask your questions or request a quote.

ELISTAR HYPERNOVA for ELISA

ELISTAR substrates is the patented product line of Cyanagen and specifically made for ELISA detection. ELISTAR HYPERNOVA is the brightest HRP ECL substrate on the market to date. The substrate is extremely sensitive and its light output is extraordinary, allowing detection of trace amounts of proteins. Low background levels result is a very high signal-to-noise ratio. 

Features

  • Trace amounts of protein detectable
  • Short exposure time
  • Less antibody and/or sample is needed

Download the Brochure

Other available ELISTAR substrate kits are:

  • ELISTAR Sun
  • ELISTAR Nova 2.0
  • ELISTAR ETA C .20
  • ELISTAR Eta C Ultra 2.0
  • ELISTAR Supernova

Download the Brochure

Ask your questions or request a quote.

HTRF (Homogenous Time Resolved Fluorescence) QuickAllAssay by BioNobile 

BioNobile’s QuickAllAssay™ kit covers all reagents needed for labelling proteins with europium chelate. The kit can be used for both homogenous and enhanced separation-based time-resolved fluorescence (TRF) assays.

Features

  • One kit for all assay formats
  • One kit for labelling proteins & peptides
  • One kit for building both homogenous and separation based TReF assays
  • Labelling has excellent biocompatibility with almost no effect on assay kinetics
  • Little to no background
  • No interference from autofluorescence or phosphorescence
  • Compatible with end point and kinetic assays

TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer)
TR-FRET includes two fluorophore labels; Eu chelate donor and a fluorescent acceptor, in close proximity of each other. TR-FRET uses the long decay Eu donor to improve the FRET energy transfer. The acceptor is activated to emit light; usually between 570 – 740 nm. 

Separation-based TReF (Time-Resolved enhanced Fluorescence assays)
A coated plate or another solid phase is needed to bind and separate one component of the assay. The bound Eu-complex fluorescence is measured after a dissociative enhancement. A second chelating reagent is added to recapture all Eu and reform a new highly fluorescent chelate. 

qFM (Fluorescence Modulation) TRF
This assay only needs one labelled component, usually a Eu-chelate attached to a ligand. Both end point as well as kinetic assays can be measured with this technique. A proprietary assay buffer modulates any free chelate fluorescence enabling direct measurement of only the bound ligand-receptor or ligand-cell complex.

LEARN MORE ABOUT LABELLING CHEMISTRY

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