HTRF (Homogenous Time Resolved Fluorescence) QuickAllAssay by BioNobile
BioNobile’s QuickAllAssay™ kit covers all reagents needed for labelling proteins with europium chelate. The kit can be used for both homogenous and enhanced separation-based time-resolved fluorescence (TRF) assays.
- One kit for all assay formats
- One kit for labelling proteins & peptides
- One kit for building both homogenous and separation based TReF assays
- Labelling has excellent biocompatibility with almost no effect on assay kinetics
- Little to no background
- No interference from autofluorescence or phosphorescence
- Compatible with end point and kinetic assays
TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer)
TR-FRET includes two fluorophore labels; Eu chelate donor and a fluorescent acceptor, in close proximity of each other. TR-FRET uses the long decay Eu donor to improve the FRET energy transfer. The acceptor is activated to emit light; usually between 570 – 740 nm.
Separation-based TReF (Time-Resolved enhanced Fluorescence assays)
A coated plate or another solid phase is needed to bind and separate one component of the assay. The bound Eu-complex fluorescence is measured after a dissociative enhancement. A second chelating reagent is added to recapture all Eu and reform a new highly fluorescent chelate.
qFM (Fluorescence Modulation) TRF
This assay only needs one labelled component, usually a Eu-chelate attached to a ligand. Both end point as well as kinetic assays can be measured with this technique. A proprietary assay buffer modulates any free chelate fluorescence enabling direct measurement of only the bound ligand-receptor or ligand-cell complex.