Plate reading solutions

Affordable, flexible plate reader

One of our commonly sold readers is the SPECTROstar Nano. This is our flexible, dedicated absorbance plate reader for relatively simple assays like Bradford protein detection, ELISA’s, enzyme kinetics and DNA and protein quantification. We also offer a Nanodrop like option to measure DNA, RNA and proteins in down to 2 µl of sample in high throughput. We have a lot of experience in this application field since we also offer the Nanodrop. Sometimes even, fluorescent techniques like Quant-IT are advised because of the limitations of Absorbance based detections. Here, we can offer our fluorescence based plate readers.

High end solutions

What we see in the field is that more and more high-end applications like Fluorescence Polarisation (FP), HTRF, Alpha and Cell based assays are performed on our plate readers. Our CLARIOstar Plus fits these assays perfectly due to its extremely high sensitivity. 

Fluorescence Polarisation

Fluorescence Polarisation (FP) for instance, is a unique and relatively easy technique to determine molecular interactions like protein/protein binding and protein/DNA binding, as well as enzyme activity. Other applications include the analysis of membrane fluidity in liposomes and mitochondrial membranes, and enzymatic assays such as proteolysis, RNA synthesis and ubiquitin conjugation/deconjugation in real time. Fluorescence Polarisation is also used to read our IgG quantification well plates. These 96- or 384- well plates are coated with probes and is based on a homogeneous, single fluorescent label strategy. No sample preparation, additional reagent or washing steps are required.

FP enables direct monitoring of the free/bound ratio of a tracer in solution in real-time. It can be used as end-point detection, equilibrium analysis with very low concentrations (typically down to the picomolar range). FP can also be used for association/ dissociation kinetics.