The traditional iCIEF cartridge is limited by the small inner diameter of the separation capillary, there is always an unmet need of higher absorbance detection sensitivity in Capillary Electrophoresis. The detection sensitivity of an imaging CIEF detection depends on both detection wavelength as well as the inner diameter of the separation column.
We provide a customised imaging CIEF detection at 280nm that gives you about 5 folds higher sensitivity detection in comparison to other imaging and conventional CIEF detection at 214nm. In addition our High Performance Imaging CIEF cartridges are 200µm ID, which provides you 100% sensitivity improvements over 100µm ID (standard use with other iCIEF instruments) and 300% over 50µm that are standard use in traditional CIEF assays.
The High Performance Imaging CIEF cartridges are available in four types of different coatings for your assays. The great advantage if using PA and AD coatings, there is no need of methyl cellulose additive makes your Research/QC work simpler in comparison to the standard FC (i)CIEF coating.
The CEInfinite High Performance Imaging CIEF cartridges are the world’s first WCID imaging cartridges enables direct coupling to a mass spectrometer.
In addition we offer many and different column coatings to be used for iCIEF assays, such as special coatings for hydrophilic and hydrophobic molecules besides the fluorocarbon coating that is typical used for general iCIEF applications.
Our new permanent methylcellulose coated cartridges enables you to perform iCIEF free from polymers. Therefore, the characterisation of any protein drug product is now possible straight forward.
Discover the benefits you would have:
In addition, the iCIEF separation in the MC coated cartridge can be compared with the routine coated cartridge of the fluorocarbon (FC), to ensure consistency in drug discovery.
Comparison FC versus MC coated cartridge
The MC coated capillary cartridge has applied to iCIEF-MS for characterising protein charged variants with reliable identification of MS after iCIEF separation:
As a result, we have now a breakthrough in iCIEF-MS to greatly improve the compatibility with MS.