CEInfinite High Sensitive Detection

CEInfinite High Sensitive Detection by using High Performance icIEF Cartridges

The traditional icIEF cartridge is limited by the small inner diameter of the separation capillary, there is always an unmet need of higher absorbance detection sensitivity in Capillary Electrophoresis. The detection sensitivity of an icIEF detection depends on both detection wavelength as well as the inner diameter of the separation column.

We provide a customised icIEF detection at 280nm that gives you about 5 folds higher sensitivity detection in comparison to other imaging  and conventional CIEF detection at 214nm. In addition our High Performance icIEF cartridges are 200µm ID, which provides you 100% sensitivity improvements over 100µm ID (standard use with other icIEF instruments) and 300% over 50µm that are standard use in traditional CIEF assays.

The High-Performance icIEF cartridges are available in five types of different coatings for your assays. The great advantage if using Next Generation CS, MC, AD and PA coatings, there is no need of methyl cellulose additive makes your Research/QC work simpler in comparison to the standard FC (i)cIEF coating. Using our Next generation icIEF coatings in combination with our critical reagents (AESlyte ampholytes and pI markers) no need for solubilizers, which are needed with the traditional icIEF.

 

Contact us for more information, let's discuss the best approach for your research together!

Main Benefits of the High Sensitive icIEF Cartridges

Our critical reagents (AESlytes ampholytes and pI markers) and capillary coatings (Next Generation) are redefining icIEF analytical methodologies and broadening their applicability for advanced icIEF fractionation, cutting-edge icIEF-MS online coupling, and novel applications for protein bio-interactions. Learn more at - Recent developments of icIEF technology for in-depth biopharmaceutical characterization .

Traditional icIEF

Fluorocarbon-based Capillary Coating (FC)

  • Highly reliable traditional hydrophobic coating
  • Suitable for pH 2-12
  • Methyl cellulose additive is necessary
  • Size I.D. 100 and 200µm
  • For icIEF QC

Next Generation icIEF

NEW! Cross-linked Silicone Capillary Coating (CS)

  • Highly reliable hydrophobic coating
  • Suitable for pH 2-12
  • No methylcellulose additive is necessary
  • For icIEF QC, fractionation and online icIEF-MS

Methylcellulose Capillary Coating (MC)

  • Avoid the need for using MC during icIEF analysis including pre-rinse and dynamic reagent added
  • Suitable for pH 2-12
  • For icIEF QC, fractionation and online icIEF-MS

Acrylamide Derivative Capillary Coating (AD)

  • Highly reliable hydrophilic coating
  • Suitable for pH 2-12
  • No methylcellulose additive is necessary
  • For icIEF QC, fractionation and online icIEF-MS

Polyacrylamide Capillary Coating (PA)

  • Highly reliable hydrophilic coating
  • Suitable for pH 2-12
  • No methylcellulose additive is necessary
  • For icIEF QC, fractionation and online icIEF-MS

Overview of the CEInfinite High Performance icIEF Cartridges

The CEInfinite High Performance icIEF cartridges are the world’s first WCID imaging cartridges enables direct coupling to a mass spectrometer.

In addition we offer many and different column coatings to be used for icIEF assays, such as special coatings for hydrophilic and hydrophobic molecules besides the fluorocarbon coating that is typical used for general icIEF applications.

Learn more about: Implementation of Highly Efficient Protein Characterization and Preparative icIEF

  • These cartridges deliver high sensitivity and performance.
  • The easy flow along the capillary makes for simpler, faster sample injection with less clogging.
  • Available in various inner diameters for better sensitivity and capacity.

Please note that these can be used with other icIEF instruments. For additional information, please do not hesitate to contact us.

Contact us for any question, let's discuss it together!

Permanent Methylcellulose coated cartridges 

Our new permanent Methylcellulose coated cartridges enables you to perform icIEF free from polymers. Therefore, the characterisation of any protein drug product is now possible straight forward.

Discover the benefits you would have: 

  • Free Methylcellulose in pre-rinse and sample running.
  • High repeatability.
  • High separation efficiency.

In addition, the icIEF separation in the MC coated cartridge can be compared with the routine coated cartridge of the fluorocarbon (FC), to ensure consistency in drug discovery.

Comparison FC versus MC coated cartridge

The MC coated capillary cartridge has applied to icIEF-MS for characterising protein charged variants with reliable identification of MS after icIEF separation:

  • Simple the operation steps.
  • Prevent contamination of MS ESI.

As a result, we have now a breakthrough in icIEF-MS to greatly improve the compatibility with MS.

Custom Made Solution Services

Custom made cartridges with different coatings and/or ID can be offered upon request. Please contact us for further support on this custom made solution services.

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support@isogen-lifescience.com

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