CEInfinite icIEF Instruments

Isogen LifescienceProductsInstrumentsNext Generation icIEF CEInfinite icIEF Instruments

CEInfinite iCIEF Instruments

Fast and in-depth characterization under a variety of different conditions including formulation and stability.

Our CEInfinite is a next generation cIEF system based on a Whole Column Time-resolved Imaging Detection (WCTID). It is standard equipped with a high sensitivity CMOS imaging sensor and powerful deep UV LED light source.

The icIEF-Analytical simplify the protein charge analysis and provide you the highest resolution and separation. The icIEF fractionation and icIEF-MS modes allows you to characterize the post-translational modifications (PTMs) and chemical degradation underlying each protein charge isomer.  The fractionated fractions can be used for your formulation and stability studies, such as peptide mapping, amino acid sequencing, binding studies (SPR, ELISA,..), potency studies and other biological assays

One icIEF workflow for your fast and in-depth product characterization

Scan rates are over 100 times faster compared to any other imaging time-resolved cIEF systems, which enables the study of:

  • Fast dynamic processes more accurately. 
  • Empowering Taylor dispersion rapid measurement of protein hydrodynamic radius.
  • Protein interaction studies.
  • Preparative time-resolved icIEF.
  • Online (native) real-time icIEF-MS.

Analytical profiling, Method Transfer - Existing CIEF Methods

Existing cIEF methods from most icIEF instruments can be transferred to CEInfinite Analytical instrument with no additional method development using the traditional cIEF FC coated capillaries.

The combination of the CEInfinite high sensitive detection and the use of the CEInfinite High Performance Imaging CIEF Cartridges (Patent Pending) and the AESlyte ampholytes the resolution and separation can be significantly improved.

Analytical profiling, Method Transfer - Existing CIEF Methods 

Your Advantages – the use of CEInfinite Instrument

icIEF is the industry golden standard for protein charge analysis. The CEInfinite icIEF instrument overcomes low analytical throughput and difficult method development associated with traditional CIEF, gel electrophoresis and other available icIEF instruments:

  • User-friendly interface, reliable operation control, highly efficient data processing, choose from CEInsight, Chromeleon or Empower (coming soon) Platform
  • Fast and simplify the protein charge variant analysis
  • Provide you consistency, repeatability and building platform methods
  • AESlyte ampholytes and special coated cartridges for complex proteins characterization (such as mAb, ADC, Fusion Proteins, Vaccines, mRNA, LNP and many more)
  • Easy method transfers and the utmost flexibility in method development
  • One icIEF workflow for your fast and in-depth protein related product characterization (analytical profiling, fractionation, and online icIEF-MS)
  • Fast identification of PTMs (online icIEF-MS)
  • Fractionated fractions are suitable for your formulation and stability studies, such as peptide mapping, amino acid sequencing, binding studies (SPR, ELISA,..), potency studies and other biological assays
  • High performance CMOS imaging sensor warrants overall system compactness, energy saving and robustness
  • Full automation for fast injection and wash cycles
  • CE and FCC certified to guarantee your safe operation

 

Whole Column Time-resolved Imaging Detection (WCTID) Technology - High Resolution Separation

The WCTID capillary electrophoresis (CE) systems have revolutionised protein charge related heterogeneity analysis with capillary isoelectric focusing (CIEF). 

  • Faster method development
  • Higher detection sensitivity
  • Better reliability
  • Higher analytical throughput

The CEInfinite icIEF is your total solution for high resolution CIEF assays. The CEInfinite icIEF instrument is standard equipped with a high sensitivity CMOS imaging sensor and powerful deep UV LED light source.

We recommend to use them in combination with the CEInfinite High Performance Imaging CIEF Cartridges (Patent Pending) and the CEInfinite AESlytes CAs, which are specially developed and synthesised for high resolution CIEF assays. 

CEInfinite icIEF High Resolution Fractionation

WCTID CIEF peak fractionation is impossible until the availability of the AES’ Proprietary Cartridge Technology. WCTID CIEF is normally conducted with a short capillary about 5 cm in length. A long enough outlet transfer capillary connected to the separation capillary in WCTID CIEF is necessary to introduce separated proteins into fractionation collection vials. This is impossible to realise with the traditional WCID CIEF cartridges. The resolution achieved in the short separation capillary will be lost during the transfer process when the focused proteins are forced into the transfer capillary.

The CEInfinite Preparative icIEF instrument provides you an unique, reliable and high resolution protein charged based separation for biologics. Characterisation of individual peaks is essential for comprehending the nature of product isomers and protein degradation pathway. Therefore it is  desirable to perform peak fractionation or direct coupling to mass spectrometer for providing structure characterisation of the separated protein isomer.

  • Fractionation Purity:

    The proteins with a difference in pI of 0.2 or greater can be fractionated with greater than 80% purity. Proteins with a difference in pI of 0.1 can be fractionated with greater than 60%. 

 

  • High Resolution Time-resolved Imaging CIEF Fractionation - AFSC

    Below figure shows a high resolution time-resolved imaging CIEF fractionation, fraction collection and fraction CIEF of haemoglobin control AFSC, demonstrating the speed and efficiency of the CEInfinite Preparative icIEF instrument.

CEInfinite icIEF-MS

WCID CIEF direct coupling to MS is impossible until the availability of the AES’ Proprietary Cartridge Technology. WCID CIEF is normally conducted with a short capillary about 5 cm in length. A long enough outlet transfer capillary connected to the separation capillary in WCID CIEF is necessary to introduce separated proteins into MS interface. This is impossible to realise with the conventional WCID CIEF cartridges.

The CEInfinite Preparative icIEF instrument besides high resolution fractionation enables also direct coupling to MS (patent pending). Protein CIEF separation and mobilisation are monitored with time-resolved imaging of the whole separation capillary, so that high resolution separation of proteins is ensured during focusing and transportation. Complex proteins are separated first according to their isoelectric point in our uniquely built CEInfinite High Performance Imaging CIEF Cartridge and then the separated proteins are forced into the transfer capillary that is connected to the ion source of the mass spectrometer for protein characterisation.

 

Protein Charged based Separation for Biologics

WCTID CIEF coupled to mass spectrometer (MS) has provided unique, reliable and high resolution protein charged based separation for biologics. Characterisation of individual peaks is essential for comprehending the nature of product isomers and protein degradation pathway. Therefore it is very desirable if WCTID CIEF can be coupled to MS or peak fractionation to provide structure characterisation of the separated protein isomer.

CEInfinite use for MALDI Target Plate

Thanks to the long and small transfer capillary, the CEInfinite Preparative iCIEF instrument allows also to spot the separated protein elution to a MALDI target plate.

NEW!  We are pleased to announce our CEInfinite Chromeleon Driver integration of the CEInfinite icIEF and icIEF-MS.

It allows you to work in a well control laboratory environment and streamlining workflow while meeting 21 CFR Part 11 compliance.

CHAMALEON CEINFINITE AND IICIEF MS

 

Besides iCIEF-orbitrap HRMS using nano-ESI (Easy Spray), we have developed the nano-Emitter. It has been developed for high-efficient characterization of protein drug heterogeneity, which achievement has been published in Analytical Biochemistry*

 

*Imaged capillary isoelectric focusing tandem high-resolution mass spectrometry using nano electrospray ionization for protein heterogeneity characterization, Analytical Biochemistry, 2023, 680, 115312  

 

Have a look at our publications!

Customer reviews

Customer review on CEInfinite icIEF 

 

CEInfinite icIEF instrument enabled us to verify easily and quickly the behavior of the pI markers we have proposed.

The operation with the instrument is easy and the time for the separation in the order of minutes, while the same focusing task performed in the classical CE capillary equipment with pressure mobilization took hours.

 

 

Customer review on CEInfinite icIEF

"The CEInfinite enables direct coupling of icIEF to mass spectrometry, which is a powerful tool for the characterization of proteoforms on the intact level.

The obtained high resolution mass spectra are of excellent quality - For me a surprisingly high quality even for intact mAbs although we use generic separation methods (standard ampholytes)."

Prof. Dr. Christian Neusüß

Hochschule Aalen, Germany

 

Customer review on CEInfinite icIEF-MS

"icIEF plays a critical for monitoring charge variants during biopharmaceuticals. The CEInfinite allows us to get a massa readout for the product variants detected by icIEF, and this is a great help for understanding the clinical nature of the charge variants. 

When combining the CEInfinite with high resolution Orbitrap MS, detailed insights into product variants and product stability can be achieved. In short, icIEF MS during the CEInfinite is a very powerful characterisation tool in biopharmaceutical development."

Dan Bach Kristensen (PhD, Principal Scientist)

Symphogen A/S, Denmark 

Watch Webinar - Using direct CIEF-MS and PREP-CIEF

Do you want to know what we do with your data from this form? Then read our privacy statement.

Request a quotation

Request a quotation for your selection of laboratory instruments, reagents, kits or consumables. We will get back to you via mail or phone in no more than 24 hours. Promised!

Do you want to know what we do with your data from this form? Then read our privacy statement.

Contact information

+31 30 6880771
support@isogen-lifescience.com

Our office

Veldzigt 2A
3454 PW De Meern

ISOgen office
Isogen Lifescience B.V.

Veldzigt 2A
3454 PW De Meern

 

Do you want to be aware of all the updates, new solutions, offers and application fields?

Sign up for our mailings!

Contact us

+31 30 6880771
info@isogen-lifescience.com

 

Follow us on social media

linkedin logo

© Isogen 2020. All rights reserved.

Website by eResults