CEInfinite iCIEF Instruments

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CEInfinite iCIEF Instruments

Our CEInfinite is a new generation CIEF system based on a Whole Column Time-resolved Imaging Detection (WCTID) 

It is standard equipped with a high sensitivity CMOS imaging sensor and powerful deep UV LED light source.

MS-Friendly

Furthermore, it is the only WCTID CIEF system that enables you to perform direct coupling to MS for characterisation studies and high resolution iCIEF fractionation for further studies like peptide mapping, SPR and biological assays. 

 

Do you want to experience our next generation iCIEF instruments?

They are 100% compatible with your current solution: Have a look at CEInfinite the brochure

Contact us for a demo!

Save time with our iCIEF Instruments

Scan rates are over 100 times faster compared to any other imaging time-resolved CIEF systems, which enables the study of:

  • Fast dynamic processes more accurately. 
  • Empowering Taylor dispersion rapid measurement of protein hydrodynamic radius.
  • Protein interaction studies.
  • Preparative time-resolved iCIEF.

Method Transfer - Existing CIEF Methods

Existing CIEF methods from most iCIEF instruments can be transferred to CEInfinite Analytical instrument with no additional method development using the traditional CIEF FC coated capillaries. However, due to the CEInfinite high sensitive detection and the use of the CEInfinite High Performance Imaging CIEF Cartridges (Patent Pending) the resolution can be significantly improved. 

We provide you with guaranteed critical iCIEF product supplies for your product lifecycle. Have a look at more specific info about it!

  • 100% Compatibility: Same WCID-iCIEF principle, same operation and one autosampler
  • High sensitivity, high resolution, high reproducibility
  • Longer UV source lifetime and extremely low energy consumption
  • Direct method transfer from iCE280 and iCE3
  • High performance robustness

Contact us for any question, let's discuss it together!

Comparison WCID-iCIEF System Specifications

 

Comparison iCIEF results with mAb1-A

 

Reduced fused protein standard method

 

Main Benefits of the CEInfinite Instrument

The CEInfinite iCIEF instrument overcomes low analytical throughput and difficult method development associated with 
traditional CIEF and gel electrophoresis:

  • High resolution separation and reproducible peak patterns.
  • WCTID-based CIEF instrument for high throughput and reliable protein separation.
  • High performance CMOS imaging sensor warrants overall system compactness, energy saving and robustness
  • Reproducible results thanks to a world-class high voltage power supply .
  • Digitalised with 14 bit ADC to improve overall detection dynamic range
  • Automated    analyser: integration    with    state-of-the-art    high    throughput autosampler for fast injection and wash cycles.
  • FDA title 21 CFR Part 11 compliance
  • User friendly interface, reliable operation control, highly efficient data processing: Standard supplied with CEInsight CFR control and quantitation software
  • CE and FCC certified to guarantee your safe operation

Whole Column Time-resolved Imaging Detection (WCTID) Technology - High Resolution Separation

The WCTID capillary electrophoresis (CE) systems have revolutionised protein charge related heterogeneity analysis with capillary isoelectric focusing (CIEF). 

  • Faster method development
  • Higher detection sensitivity
  • Better reliability
  • Higher analytical throughput

The CEInfinite iCIEF is your total solution for high resolution CIEF assays. The CEInfinite iCIEF instrument is standard equipped with a high sensitivity CMOS imaging sensor and powerful deep UV LED light source.

We recommend to use them in combination with the CEInfinite High Performance Imaging CIEF Cartridges (Patent Pending) and the CEInfinite AESlytes CAs, which are specially developed and synthesised for high resolution CIEF assays. 

CEInfinite iCIEF High Resolution Fractionation

WCTID CIEF peak fractionation is impossible until the availability of the AES’ Proprietary Cartridge Technology. WCTID CIEF is normally conducted with a short capillary about 5 cm in length. A long enough outlet transfer capillary connected to the separation capillary in WCTID CIEF is necessary to introduce separated proteins into fractionation collection vials. This is impossible to realise with the traditional WCID CIEF cartridges. The resolution achieved in the short separation capillary will be lost during the transfer process when the focused proteins are forced into the transfer capillary.

The CEInfinite Preparative iCIEF instrument provides you an unique, reliable and high resolution protein charged based separation for biologics. Characterisation of individual peaks is essential for comprehending the nature of product isomers and protein degradation pathway. Therefore it is  desirable to perform peak fractionation or direct coupling to mass spectrometer for providing structure characterisation of the separated protein isomer.

  • Fractionation Purity:

    The proteins with a difference in pI of 0.2 or greater can be fractionated with greater than 80% purity. Proteins with a difference in pI of 0.1 can be fractionated with greater than 60%. 

 

  • High Resolution Time-resolved Imaging CIEF Fractionation - AFSC

    Below figure shows a high resolution time-resolved imaging CIEF fractionation, fraction collection and fraction CIEF of haemoglobin control AFSC, demonstrating the speed and efficiency of the CEInfinite Preparative iCIEF instrument.

CEInfinite iCIEF-MS

WCID CIEF direct coupling to MS is impossible until the availability of the AES’ Proprietary Cartridge Technology. WCID CIEF is normally conducted with a short capillary about 5 cm in length. A long enough outlet transfer capillary connected to the separation capillary in WCID CIEF is necessary to introduce separated proteins into MS interface. This is impossible to realise with the conventional WCID CIEF cartridges.

The CEInfinite Preparative iCIEF instrument besides high resolution fractionation enables also direct coupling to MS (patent pending). Protein CIEF separation and mobilisation are monitored with time-resolved imaging of the whole separation capillary, so that high resolution separation of proteins is ensured during focusing and transportation. Complex proteins are separated first according to their isoelectric point in our uniquely built CEInfinite High Performance Imaging CIEF Cartridge and then the separated proteins are forced into the transfer capillary that is connected to the ion source of the mass spectrometer for protein characterisation.

 

Protein Charged based Separation for Biologics

WCTID CIEF coupled to mass spectrometer (MS) has provided unique, reliable and high resolution protein charged based separation for biologics. Characterisation of individual peaks is essential for comprehending the nature of product isomers and protein degradation pathway. Therefore it is very desirable if WCTID CIEF can be coupled to MS or peak fractionation to provide structure characterisation of the separated protein isomer.

CEInfinite use for MALDI Target Plate

Thanks to the long and small transfer capillary, the CEInfinite Preparative iCIEF instrument allows also to spot the separated protein elution to a MALDI target plate.

NEW!  We are pleased to announce our CEInfinite Chromeleon Driver integration of the CEInfinite iCIEF and iCIEF-MS.

It allows you to work in a well control laboratory environment and streamlining workflow while meeting 21 CFR Part 11 compliance.

CHAMALEON CEINFINITE AND IICIEF MS

 

Besides iCIEF-orbitrap HRMS using nano-ESI (Easy Spray), we have developed the nano-Emitter. It has been developed for high-efficient characterization of protein drug heterogeneity, which achievement has been published in Analytical Biochemistry*

 

*Imaged capillary isoelectric focusing tandem high-resolution mass spectrometry using nano electrospray ionization for protein heterogeneity characterization, Analytical Biochemistry, 2023, 680, 115312  

 

Have a look at our publications!

Customer reviews

Customer review on CEInfinite iCIEF 

 

CEInfinite iCIEF instrument enabled us to verify easily and quickly the behavior of the pI markers we have proposed.

The operation with the instrument is easy and the time for the separation in the order of minutes, while the same focusing task performed in the classical CE capillary equipment with pressure mobilization took hours.

 

 

Customer review on CEInfinite iCIEF

"The CEInfinite enables direct coupling of iCIEF to mass spectrometry, which is a powerful tool for the characterization of proteoforms on the intact level.

The obtained high resolution mass spectra are of excellent quality - For me a surprisingly high quality even for intact mAbs although we use generic separation methods (standard ampholytes)."

Prof. Dr. Christian Neusüß

Hochschule Aalen, Germany

 

Customer review on CEInfinite iCIEF-MS

"iCIEF plays a critical for monitoring charge variants during biopharmaceuticals. The CEInfinite allows us to get a massa readout for the product variants detected by iCIEF, and this is a great help for understanding the clinical nature of the charge variants. 

When combining the CEInfinite with high resolution Orbitrap MS, detailed insights into product variants and product stability can be achieved. In short, iCIEF MS during the CEInfinite is a very powerful characterisation tool in biopharmaceutical development."

Dan Bach Kristensen (PhD, Principal Scientist)

Symphogen A/S, Denmark 

Watch Webinar - Using direct CIEF-MS and PREP-CIEF

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