CEInfinite iCIEF Solutions

CEInfinite AESlytes Carrier Ampholytes

Carrier ampholytes are essential for isoelectric focusing (IEF) separation of proteins. The fundamental requirements of good Carrier ampholytes for IEF applications are good pH linearity, high buffering capacity, small ΔpK (less than 4), good stability, and insignificant influence on the sample. 

Since early Carrier ampholytes were developed before the introduction of imaged capillary IEF (iCIEF), the unique requirement of low UV absorbance could not be applied in iCIEF application. In addition, interaction of Carrier ampholytes with protein in gel media and solution phase is different. Therefore, it is advantageous to have Carrier ampholytes for iCIEF with low protein interaction in solution phase for reproducible iCIEF protein separation and with low UV absorbance for minimised electropherogram baseline noise.

The AESlytes Carrier ampholytes were developed specifically for protein high performance iCIEF separation based on our extensive research.

In addition to meet all the requirements of good Carrier ampholytes, such as 

  • good pH linearity
  • high buffering capacity
  • good stability
  • low protein interaction
  • low UV absorbance

AESlytes are composed of a remarkably high percentage of effective Carrier ampholytes (CAs) with small ΔpK, which provides faster focusing and more stable pH gradient. Proteins are so complex, and they react differently with CAs, depending on the functional groups of CAs. Therefore, we developed three series of AESlytes that possess different basic functional groups to minimise protein interaction. AESlytes are available in High Resolution (HR), Super High (SH) and Ultra High (UH) resolution series.

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Overview of the CEInfinite AESlytes Carrier Ampholytes

We promise our researchers to provide them consistent quantity and quality of AESlytes CAs. 

CEInfinite AESlytes CAs are synthesised strictly to ensure a high performance iCIEF separation. Due to their low interaction with proteins increases resolving power and CEInfinite AESlytes CAs do not strongly absorb UV and eliminating baseline noise. Finally, their linear pH gradient makes for an outstanding accurate pI calibration.

Overview of other CEInfinite Solutions

All CEInfinite test kits, solutions are fully tested and certified. The products are manufactured under strict quality control guidelines to save you time as well as eliminating variability for a more reproducible result.

Please note that the CEInfinite AESlytes CAs and other solutions can be used for any iCIEF as well as for CIEF assays. 

If you need any additional information, please do not hesitate to contact us.

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Custom Made Solution Services

We understand the complexity of a protein. To achieve higher resolution and better separation of your current (i)CIEF assays, we also offer custom made AESlytes CAs upon request. Please contact us for further support on this custom-made solution services.

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