CEInfinite AESlytes Carrier Ampholytes
Carrier ampholytes are essential for isoelectric focusing (IEF) separation of proteins.
Since early Carrier ampholytes were developed before the introduction of imaged capillary IEF (iCIEF), the unique requirement of low UV absorbance could not be applied in iCIEF application. In addition, interaction of Carrier ampholytes with protein in gel media and solution phase is different. Therefore, it is advantageous to have Carrier ampholytes for iCIEF with low protein interaction in solution phase for reproducible iCIEF protein separation and with low UV absorbance for minimised electropherogram baseline noise.
AESlytes are composed of a remarkably high percentage of effective Carrier ampholytes (CAs) with small ΔpK, which provides faster focusing and more stable pH gradient.
Proteins are so complex, and they react differently with CAs, depending on the functional groups of CAs. Therefore, we developed three series of AESlytes that possess different basic functional groups to minimise protein interaction. AESlytes are available in High Resolution (HR), Super High (SH) and Ultra High (UH) resolution series.