Detection of a functionally deficient AAT protein variant by protease inhibitor (PI) typing
Alpha-1-antitrypsin deficiency (AATD) is a rare hereditary condition resulting from the mutations in the SERPINA1 (serine protease inhibitor) gene and is characterized by low circulating levels of the alpha-1 antitrypsin (AAT) protein. The traditional algorithm for laboratory testing of AATD involves the analysis of AAT concentrations (nephelometry), phenotyping (isoelectric focusing, IEF), and genotyping (polymerase chain reaction, PCR).
For IEF the ampholyte HR 4.2-4.9 is required. Did you know that Isogen Life Science is the only provider for this ampholyte?
If you are struggling with your assays and need customised ampholytes, we can help you with this request. Let us work together.
Both HR and SH CEInfinite AESlytes are good for icIEF of Monoclonal Antibodies (mAbs) and Antibody Drug Conjugates (ADCs). Please contact us for further information on the materials, methods, and data analysis.
mAb
ADC
Both HR and SH CEInfinite AESlytes are good for icIEF profiling in dairy proteins, meat proteins, and seed proteins.
Please contact us for further information on the materials, methods, and data analysis.
Casein Profiling in Meat
Whey Profiling in Meat
Protein Profiling in Meat and Related Products
UH CEInfinite AESlytes are optimised for analysing complex proteins such as Fusion Proteins, AAV8 as well as enzymes with multiple sialic acids. Just like our HR and SH series, they are extremely stable, have low UV 280 nm absorbance and with good lot to lot consistency. Please contact us for further information on the materials, methods, and data analysis.
For your lifecycle CMC strategy, contact us to learn more about our critical reagent service.
Fusion Proteins
An AAV8 Empty Capsid
CEInfinite AESlytes have demonstrated superior focusing and low UV noise. The below figure illustrated focusing of 6 pI markers with CEInfinite AESlyte SH pH 4-8 and pH 3-7 from another vendor. Much higher baseline noise was observed from the electropherogram with CAs from the other vendor.
Furthermore the pI 5.85 did not complete focusing with CAs from the other vendor under the same experimental conditions.
The CEInfinite Preparative instrument allows you also to perform offline icIEF-MS by High Yield Fractionation besides fast protein profiling. The collected fractions can be further analysed for bio and cellular assays (such as SPR, ELISA, Potency, Peptide mapping and more). Furthermore it allows you to perform online icIEF-MS for fast identification of the PTMs, DARs, (im)purities and/or mass confirmation.
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