CEInfinite iCIEF Instruments

Name: Isogen Life Science B.V.
Address: Veldzigt 2a, De Meern, 3454 PW, NL
Telephone: +31 30 6880771

The CEInfinite is a new generation Whole Column Time-resolved Imaging Detection (WCTID) CIEF system, standard equipped with a high sensitivity CMOS imaging sensor and powerful deep UV LED light source. CEInfinite dramatically increases the scan rate over 100 times compared to any other imaging time-resolved CIEF systems. An increased scan rate enables the study of:

Fast dynamic processes more accurately.
Empowering Taylor dispersion rapid measurement of protein hydrodynamic radius.
Protein interaction studies.
Preparative time-resolved imaging CIEF.

Furthermore, it is the only WCTID CIEF system that allows you to perform direct coupling to MS for characterisation studies and high resolution iCIEF fractionation for further studies like peptide mapping, SPR and biological assays.

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Method Transfer - Existing CIEF Methods

Existing CIEF methods from most iCIEF instruments can be transferred to CEInfinite Analytical instrument with no additional method development using the traditional CIEF FC coated capillaries. However, due to the CEInfinite high sensitive detection and the use of the CEInfinite High Performance Imaging CIEF Cartridges (Patent Pending) the resolution can be significantly improved.
Please do not hesitate to contact us for additional information and support on your methods transfer.

CEInfinite System Advantages
Comparison WCID-iCIEF System Specifications
Comparison iCIEF results

Isogen Life Science is also a supplier that provides you guaranteed critical iCIEF product supplies for your product lifecycle.

CEInfinite System Advantages

Same WCID-iCIEF principle, same operation and one autosampler
High sensitivity, high resolution, high reproducibility
Longer UV source lifetime and extremely low energy consumption
Direct method transfer from iCE280 and iCE3
High performance robustness

Comparison WCID-iCIEF System Specifications

Comparison iCIEF results

mAb1-A

Reduced fused protein standard method

Whole Column Time-resolved Imaging Detection Technology - High Resolution Separation

Whole column time-resolved imaging detection (WCTID) capillary electrophoresis (CE) systems have revolutionised protein charge related heterogeneity analysis with capillary isoelectric focusing (CIEF). Unlike the electropherogram in the traditional single point detection CE system display absorbance at a specific point along the separation capillary at different times until completion of the CIEF separation. Compared to the traditional CE system, WCTID CE provides you

Faster method development
Higher detection sensitivity
Better reliability
Higher analytical throughput

The CEInfinite iCIEF is your total solution for high resolution CIEF assays. The CEInfinite iCIEF instrument is standard equipped with a high sensitivity CMOS imaging sensor and powerful deep UV LED light source. Using in combination with the CEInfinite High Performance Imaging CIEF Cartridges (Patent Pending) and the CEInfinite AESlytes CAs, which are specially developed and synthesised for high resolution CIEF assays.

Truly empowers Dynamic Processes Investigation with WCTID

In addition, the CEInfinite iCIEF instrument is the only system that truly empowers dynamic processes investigation with WCTID. With the application of a high sensitivity CMOS imaging sensor, scan frequency for the whole separation capillary can be as high as 1000Hz. The increased scan frequency effectively minimises imaging blur that occurs at a lower scan frequency enables dynamic processes, such as

Taylor dispersion protein hydrodynamic radius measurement,
Protein interaction studies
Preparative time-resolved imaging CIEF

The capability of the CEInfinite iCIEF instrument to scan at a high frequency truly opens a new technology of whole column detection time-resolved imaging CIEF

Customer review on CEInfinite iCIEF

CEInfinite iCIEF instrument enabled us to verify easily and quickly the behavior of the pI markers we have proposed.

The operation with the instrument is easy and the time for the separation in the order of minutes, while the same focusing task performed in the classical CE capillary equipment with pressure mobilization took hours.

CEInfinite iCIEF High Resolution Fractionation

WCTID CIEF peak fractionation is impossible until the availability of the AES’ Proprietary Cartridge Technology. WCTID CIEF is normally conducted with a short capillary about 5 cm in length. A long enough outlet transfer capillary connected to the separation capillary in WCTID CIEF is necessary to introduce separated proteins into fractionation collection vials. This is impossible to realise with the traditional WCID CIEF cartridges. The resolution achieved in the short separation capillary will be lost during the transfer process when the focused proteins are forced into the transfer capillary.

The CEInfinite Preparative iCIEF instrument provides you an unique, reliable and high resolution protein charged based separation for biologics. Characterisation of individual peaks is essential for comprehending the nature of product isomers and protein degradation pathway. Therefore it is desirable to perform peak fractionation or direct coupling to mass spectrometer for providing structure characterisation of the separated protein isomer.

Fractionation Purity

The proteins with a difference in pI of 0.2 or greater can be fractionated with greater than 80% purity. Proteins with a difference in pI of 0.1 can be fractionated with greater than 60%.

High Resolution Time-resolved Imaging CIEF Fractionation - AFSC

Below figure shows a high resolution time-resolved imaging CIEF fractionation, fraction collection and fraction CIEF of haemoglobin control AFSC, demonstrating the speed and efficiency of the CEInfinite Preparative iCIEF instrument.

CEInfinite iCIEF-MS

WCID CIEF direct coupling to MS is impossible until the availability of the AES’ Proprietary Cartridge Technology. WCID CIEF is normally conducted with a short capillary about 5 cm in length. A long enough outlet transfer capillary connected to the separation capillary in WCID CIEF is necessary to introduce separated proteins into MS interface. This is impossible to realise with the conventional WCID CIEF cartridges.

The CEInfinite Preparative iCIEF instrument besides high resolution fractionation enables also direct coupling to MS (patent pending). Protein CIEF separation and mobilisation are monitored with time-resolved imaging of the whole separation capillary, so that high resolution separation of proteins is ensured during focusing and transportation. Complex proteins are separated first according to their isoelectric point in our uniquely built CEInfinite High Performance Imaging CIEF Cartridge and then the separated proteins are forced into the transfer capillary that is connected to the ion source of the mass spectrometer for protein characterisation.

Customer review on CEInfinite iCIEF-MS

“iCIEF plays a critical for monitoring charge variants during biopharmaceutical development. The CEInfinite allows us to get a mass readout for the product variants detected by iCIEF, and this is a great help for understanding the chemical nature of the charge variants. When combining the CEInfinite with high resolution Orbitrap MS, detailed insight into product variants and product stability can be achieved. In short, iCIEF MS using the CEInfinite is a very powerful characterization tool in biopharmaceutical development.”

Customer review on CEInfinite iCIEF

"The CEInfinite enables direct coupling of iCIEF to mass spectrometry, which is a powerful tool for the characterization of proteoforms on the intact level.The obtained high resolution mass spectra are of excellent quality - For me a surprisingly high quality even for intact mAbs although we use generic separation methods (standard ampholytes)."

Protein Charged based Separation for Biologics

WCTID CIEF coupled to mass spectrometer (MS) has provided unique, reliable and high resolution protein charged based separation for biologics. Characterisation of individual peaks is essential for comprehending the nature of product isomers and protein degradation pathway. Therefore it is very desirable if WCTID CIEF can be coupled to MS or peak fractionation to provide structure characterisation of the separated protein isomer.

CEInfinite use for MALDI Target Plate

Thanks to the long and small transfer capillary, the CEInfinite Preparative iCIEF instrument allows also to spot the separated protein elution to a MALDI target plate.

Why Isogen Life Science?

At Isogen Life Science, we believe that long term relationships with life science professionals and suppliers are fundamental to solve life science problems. Our curiosity drives us to continually stay involved and to dig deep in dialogue, with both clients and suppliers. It’s these intensive collaborations that enable us to develop tailored, innovative products that push life science’s boundaries. Partner up with us and enjoy our quick support for troubleshooting and questions!

Making your research happen.

Contact information

+31 30 6880771
support@isogen-lifescience.com

Our office

Veldzigt 2A
3454 PW De Meern

CEInfinite iCIEF Instruments

CEInfinite iCIEF Instruments