CEInfinite AESlytes Carrier Ampholytes
Carrier ampholytes (CAs) are essential for isoelectric focusing (IEF) separation of proteins. The fundamental requirements of good CAs for IEF applications are good pH linearity, high buffering capacity, small ΔpK (less than 4), good stability, and insignificant influence on the sample.
Since early CAs were developed before the introduction of imaged capillary IEF (iCIEF), the unique requirement of low UV absorbance could not be applied in iCIEF application. In addition, interaction of CAs with protein in gel media and solution phase is different. Therefore, it is advantageous to have CAs for iCIEF with low protein interaction in solution phase for reproducible iCIEF protein separation and with low UV absorbance for minimised electropherogram baseline noise.