mRNA/whole transcriptome sequencing

mRNA/whole transcriptome sequencing

The Lexogen Total RNA Library Prep kits have been well established over the last years and have proven to deliver high quality RNA-seq libraries, generating best-in-class results.

Lexogen has now launched the CORALL total RNA-seq Library Prep kit. This kit shows improved performance, including reduced PCR cycle numbers for library amplification and improved coverage uniformity.

But, in addition, CORALL RNA-seq Library Prep kits succeed in consolidating your RNA-seq workflows, as the CORALL kits can be used for mRNA-seq as well as for total RNA-seq, simply by adding a specific module to treat your input material.

Product information

CORALL Total RNA-seq Library Prep Kit

CORALL is the all-in-one library prep kit for whole transcriptome sequencing, featuring seamless integration of Unique Molecular Identifiers (UMI), excellent whole transcriptome coverage and exceptional protocol-inherent strand specificity. The fragmentation-free protocol uses Lexogen’s proprietary Strand Displacement Stop and Ligation technologies to deliver complete transcript representation, including start and end sides.

When combined with the Poly-A RNA selection kit, CORALL is the first choice for mRNA-seq
o    Complete coverage of transcripts from start to end
o    Ready-to-sequence libraries within 4.5 hours
o    UMI’s seamlessly included
o    Excellent protocol-inherent strandedness (99%)
o    1ng to 1µg of total RNA input
o    Easy all-in-one protocol
o    Automation Friendly

The kit can be used for the following applications:
o    Gene Expression Profiling
o    Isoform discovery and quantification
o    Alternative splicing studies
o    Transcript (re)annotation
o    SLAMseq metabolic RNA sequencing

Flexible Input
CORALL Library prep supports an input of 1ng to 1µg of total RNA input. However, depending on the sequencing application, a pre-treatment of the input RNA is recommended.

For mRNA-seq, a pre-treatment with the Poly-A RNA selection kit is required, whereas for total RNA-seq a pre-treatment with the RiboCop rRNA depletions is strongly advised to deplete the highly abundant ribosomal RNA from your sample, enabling more cost-effective sequencing.

When using a pre-treatment method, it needs to be taken into account that 100-200ng of total RNA will result in 1-2 ng poly-A enriched or rRNA depleted RNA.

When using total RNA without any depletion or enrichment, it is possible to work with inputs ranging from 100pg to 100ng.

Gene Detection
The high level of complexity of the CORALL libraries ensure faithful representation of the transcriptome, making this kit an excellent choice for sensitive expression profiling. The excellent gene discovery rates, Corall is delivering, do match the leading competitor kits.

Transcript Coverage
Corall generates transcriptome-wide smooth and highly uniform read coverage

End-to-End coverage
CORALL’s improved and comprehensive coverage delivers best-in-class start and end site representation. Read coverage analysis, using the ERCC spike-in controls, clearly indicate that CORALL reads map more accurately to the exact ERCC TSS than competitor libraries that fail to cover the true start sites. In addition, CORALL provides superior coverage at the TES, compared to the competitor kits.

Unique Molecular Identifiers (UMI) seamlessly included
UMI’s are standardly included in the CORALL Library Prep kit and are seamlessly introduced into the libraries as an inherent part of the protocol. This results in Read 1 containing the UMI information, enabling direct access in the cost-efficient single-read mode.

Data Analysis
A Data Analysis Pipeline is now available on the BlueBee® Genomics Platform. The provided pipeline enables performing read quality control, mapping, UMI deduplication and transcript quantification.

Ordering information

It is possible to order the CORALL kit in different sizes and in combination with RiboCop.

Contact us for pricing and additional information

Art #DescriptionReaction Size

095.24

CORALL Total RNA-Seq Library Prep Kit

24 preps

095.96

CORALL Total RNA-Seq Library Prep Kit

96 preps

117.96

CORALL Total RNA-Seq Library Prep Kit with UDI 12 nt Set A1, (UDI12A_0001-0096), 1 rxn/UDI

96 preps

118.96

CORALL Total RNA-Seq Library Prep Kit with UDI 12 nt Set B1, (UDI12B_0001-0096), 1 rxn/UDI

96 preps

119.384

CORALL Total RNA-Seq Library Prep Kit with UDI 12 nt Sets A1-A4, (UDI12A_0001-0384), 1 rxn/UDI

384 preps

132.96

CORALL Total RNA-Seq Library Prep Kit with UDI 12 nt Set A2, (UDI12A_0097-0192), 1 rxn/UDI

96 preps

133.96

CORALL Total RNA-Seq Library Prep Kit with UDI 12 nt Set A3, (UDI12A_0193-0288), 1 rxn/UDI

96 preps

134.96

CORALL Total RNA-Seq Library Prep Kit with UDI 12 nt Set A4, (UDI12A_0289-0384), 1 rxn/UDI

96 preps

146.24

CORALL Total RNA-Seq Library Prep Kit with RiboCop (HMR)

24 preps

146.96

CORALL Total RNA-Seq Library Prep Kit with RiboCop (HMR)

96 preps

147.24

CORALL Total RNA-Seq Library Prep Kit with RiboCop (HMR+Globin)

24 preps

147.96

CORALL Total RNA-Seq Library Prep Kit with RiboCop (HMR+Globin)

96 preps

158.96

CORALL mRNA-Seq Library Prep Kit with UDI 12 nt Set A1, (UDI12A_0001-0096)

96 preps

159.96

CORALL mRNA-Seq Library Prep Kit with UDI 12 nt Set A2, (UDI12A_0097-0192)

96 preps

160.96

CORALL mRNA-Seq Library Prep Kit with UDI 12 nt Set A3, (UDI12A_0193-0288)

96 preps

161.96

CORALL mRNA-Seq Library Prep Kit with UDI 12 nt Set A4, (UDI12A_0289-0384)

96 preps

162.96

CORALL mRNA-Seq Library Prep Kit with UDI 12 nt Set B1, (UDI12B_0001-0096)

96 preps

163.384

CORALL mRNA-Seq Library Prep Kit with UDI 12 nt Sets A1-A4 (UDI12A_0001-0384)

384 preps

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Contact information

+31 30 6880771
support@isogen-lifescience.com

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