RNA Enrichment and Depletion - Lexogen

Name: Isogen Life Science B.V.
Address: Veldzigt 2a, De Meern, 3454 PW, NL
Telephone: +31 30 6880771

When looking at total RNA, about 80% is ribosomal RNA (rRNA) and is mostly unwanted in RNA-seq experiments. When rRNA is not removed from the sample during library prep, it will occupy most of the sequencing space, reducing the number of samples that can be pooled on a single lane of a flow cell and therefore, increase the sequencing cost dramatically.

Next to total RNA-seq, mRNA-seq is also an important application. Knowing that the mRNA-fraction only represents a few percentages of the total RNA, it is good practice to enrich for this fraction prior to mRNA library prep by selecting and capturing the poly-A tailed mRNA.

We do offer 2 different options for rRNA depletione or Poly-A selection. Combined with the CORALL total RNA-seq library prep kit, this represents a powerful and streamlined method for total RNA- and mRNA-seq

RiboCop rRNA Depletion Kit V.2 (Human/Mouse/Rat)
RiboCop rRNA Depletion Kits for Bacteria
RiboCop rRNA Depletion Kit for Yeast NEW!!
RiboCop rRNA Depletion Kit for Plants NEW!
Poly-A RNA Selection Kit

Product information

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RiboCop rRNA Depletion Kit V.2 (H/M/R)

About 80% of the total RNA after extraction is ribosomal RNA (rRNA) which is generally of little interest. The RiboCop rRNA depletion kit enables removal of rRNA from total RNA and is suited for NGS as well as other demanding RNA Analysis applications

Efficient elimination of cytoplasmic and mitochondrial rRNA
Simple workflow; no enzymatic reactions
Output ready for NGS Library Prep
Down to 1ng of total RNA input
Fast protocol: rRNA depleted in just 1,5 hours
HMR with Globin for blood RNA samples

Efficient Elimination of rRNA

RiboCop enables efficient removal of cytoplasmic 28S, 18S, 5.8S, 45S, 5S, as well as mitochondrial mt16S, mt12S ribosomal RNA sequences in human, mouse and rat samples. Sequencing depth is preserved for relevant RNA species.

Flexible Input Requirements

RiboCop is suitable for rRNA depletion of 1ng - 1µg total RNA input amounts from intact or degraded RNA, including FFPE samples

Simple Workflow

The RiboCop protocol allows rRNA depletion on only 1.5 hours, including a hands-on time of 30 minutes. It does not involve enzymatic reactions or mechanical shearing steps, leaving full-length transcripts intact for downstream processing.

Automation friendly

Magnetic beads are used for depletion and purification steps, rendering the protocol completely automation friendly.

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RiboCop rRNA Depletion Kits Bacteria

RiboCop bacteria is ideal for RNA depletion of bacteria. Bacterial RNA consists 98% of ribosomal RNA, which can make it challenging to analyse the transcriptome capacity of complex cultures. With the RiboCop rRNA depletion kit, 23S, 16S and 5S rRNA is effectively removed from mixed or monocultures.

With just 1ng to 1ug total RNA input, the rRNA depletion kit reduces rRNA to 1-3%, while maintaining unbiased transcript abundance.

The robust protocols, make it highly reproducible. Depletions without off-target effects are performed.

The RiboCop rRNA depletion kit is a stand-alone kit with the option to choose from three optimised Probe Mixes for depletion of rRNA from mixed bacterial samples (META) or from Gram negative or Gram-positive bacteria (G- or G+) grown in monoculture.

Benefits of the RiboCop rRNA depletion kit for bacteria

Ideal for complex, mixed and monocultures
Reduces rRNA to 1-3%
Unbiased transcript abundance

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RiboCop rRNA Depletion Kit for Yeast

Yeast total RNA is comprised of large amounts of undesired ribosomal RNA (rRNA) which accounts for up to ~97 % of all transcripts. Our RiboCop rRNA Depletion Kits for Yeast remove undesired cytoplasmic (25S, 18S, 5.8S, 5S, and 35S) and mitochondrial (21S, 15S, ACI60_gr01 and ACI60_gr02) rRNA from intact as well as degraded input RNA. Ribo-depletion affords an unbiased view of the transcriptome, including non-coding and non-polyadenylated RNA species.

Reduces rRNA reads to 1%
Maintains unbasied Expression Profiles
Increases Gene detection upon rRNA Depletion

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Poly-A RNA Selection Kit

The Poly-A RNA Selection Kit is the ideal solution for rapid and highly specific enrichment of polyadenylated RNAs from total RNA samples.

Highly specific poly-A enrichment
Magnetic bead-based purification
Fast workflow

Highly specific for poly-A RNA

Other RNA species (rRNA and tRNA) do not contain poly-A sequences and will not bind to the oligo(dT) beads.

Rapid Turnaround

The Poly-A RNA selection kit enables isolation of poly-A RNA from total RNA in about 1 hour.

Various downstream Applications

The enriched mRNA fraction can directly be used for RNA-seq library prep, SAGE, CAGE, cloning, micro-arrays, cDNA synthesis etc.

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RiboCop rRNA Depletion Kit for Plants

Plant total RNA is comprised of large amounts of undesired ribosomal RNA (rRNA) which accounts for up to ~97 % of all transcripts.

Our new kit for plants removes undesired cytoplasmic (5.8S, 18S, and 25S), mitochondrial (5S, 18S, and 26S), and plastid (4.5S, 5S, 16S and 23S) rRNA from intact as well as degraded input RNA. Ribo-depletion affords an unbiased view of the transcriptome, including non-coding and non-polyadenylated RNA species.

Do you work with plant material? Save sequencing costs!
🧪 An efficient and straightforward ribo-depletion from plant total RNA.
🧪 Get rid of the 97% rRNAs of all transcripts in total plant RNA.
🧪 Sequence what matters.

Demonstration of successful depletion of rRNA after treatment with RiboCop rRNA Depletion for Plants.

Figure 1 | RiboCop rRNA Depletion for Plants efficiently removes rRNA across a wide range of input amounts.

Robust Performance Over a Wide Range of Input Amounts

Ribosomal RNA was depleted from Arabidopsis thaliana total RNA using RiboCop for Plants over a wide range of input amounts (1 ng to 1 µg). RiboCop for Plants efficiently reduces rRNA reads for all input amounts (Fig. 1).

Increased Gene Detection upon rRNA Depletion

Discover the complete plant transcriptome and sequence what matters to your experiment. RiboCop for Plants allows an unobstructed view of the transcriptome and increases gene detection at 3 M reads / sample by more than 45 % through efficient rRNA removal (Fig. 2).

ribo depletion for plant NGS

The number of detected genes upon rRNA depletion of A. thaliana total RNA with with RiboCop for Plants using 1 µg or 1 ng input RNA.

Figure 2 | Increased gene detection upon rRNA depletion.

Do you work with plant material?

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