Newsletter

Important aspects in Sanger sequencing are the purification and the use of high quality products. This will eliminate any interference and increase the intensity and spacing of peaks

• Template Preparation
• Sequencing Reaction and Sequencing Clean up
• Capillary Electrophoresis and Regeneration
  • Exclusive: FREE Enhancer Sample Request

Template Preparation

The purification step for PCR products, such as DNA polymerase, dNTPs or primers can be performed with use of magnetic cleaning beads or with a simple enzymatic reaction using ExoZap.

Sequencing Reaction and Sequencing Clean up

Linear amplifications are labelled with one nucleotide difference with a ddNTP to terminate the chain. For this step, we offer the SupreDye Cycle Sequencing Kit.

Capillary Electrophoresis and Regeneration

For resuspension of your ethanol purified samples, we provide formamide or polymers. The polymers are effective for separation of extension products. It creates even peak separation for long reads.   

The enhancer can mitigate the negative effects of reduced SupreDye Ready Mix amount used in cycle sequencing reaction. Addition of the enhancer to the reaction reduces the rate of signal drop over the reading length, providing more even peak distribution. 

Request your sample of this Exclusive Product: Enhancer

After extensive usage of the capillary electrophoresis, there is a need for regeneration to avoid uneven peak space and short reading lengths. For this, we offer the Regeneration kit.