Polymerases

Name: Isogen Life Science B.V.
Address: Veldzigt 2a, De Meern, 3454 PW, NL
Telephone: +31 30 6880771

Individual polymerases

Next to our Master Mixes, we also provide (the same) polymerases individually or together with other reagents in kits.

S7 Fusion Polymerase™

S7 Fusion Polymerase™ is a robust and a high-fidelity DNA polymerase that shows remarkable results in multiple PCR applications. Fusion of the polymerase to the Sso7d domain from Sulfolobus solfataricus, which has processivity enhancing capabilities, makes the S7 Fusion Polymerase™ one of the fastest and most robust PCR polymerases on the market. Is has a 3’ to 5’ exonuclease (proofreading) activity and creates blunt ends in PCR.

Features

High performance, also with high GC content templates
Resistant to PCR inhibitors
Very high fidelity
High yields with amplicons up to 20 kb
Works with various sample materials, including direct blood

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Comparison of S7 Fusion Polymerase to another high fidelity enzyme (P), both with GC and HF buffer, and a standard Taq polymerase. A 2756 bp PCR ampliqon was analyzed by PacBio sequencing and the percentage of PCR errors is displayed in the figure above. Using high fidelity polymerases result in less errors compared to using the standard Taq polymerase and using HF buffers show best performances.