Polymerases

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DNA Polymerases: The most important component when running your PCR

We offer a variety of high quality polymerases for a wide range of applications:

Our polymerases have the glycerol free version for the TaqDNA and the TEMPase polymerase. Besides, we have different subtypes of polymerases for specific research applications.

Contact us for more specific details           We will get back to you in 1 to 2 working days

 

Our polymerases can also be found in Master Mix set up. 

Taq DNA polymerase

The main applications where the polymerase can be use are routine PCR protocols, high throughput, genotyping, cloning and colony PCR.

Features 

  • A thermostable recombinant DNA Polymerase
  • High product yield
  • dUTP incorporation possible
  • processes up to 5 kb
  • Leaves a 3'dA overhang

For the discussion of more information about the Taq DNA polymerase

Contact us

 

Our polymerase showed high specificity and good performace when targeting three different genes: PAH (203bp), ENG0 (293 bp) and ENG 5 (480 bp). When comparing it to other brands, our Taq DNA polymerase showed higher specificity when targeting longer length genes such as ENG0 and ENG5. 

TEMPase Hot Start DNA polymerase

Routine PCR, high throughout, GC-rich DNA templates, multiplex PCR, genotyping, cloning, low abundance targets, colony PCR, gene expression and microbial-pathogen detection. From all of them, we recommend to use them in applications such as  colony PCR, gene expression and microbial-pathogen detection.

Features

  • Reaction set-up at room temperature
  • dUTP incorporation possible
  • Increased sensitivity, specificity and product yield

 

In some research studies, our TEMPase Hot Start polymerase showed an increase in specificity and yield compared to the Taq polymerase. Therefore, the TEMPase is suitable for detecting low abundance targets

For the discussion of more information 

Contact us

AQ97 High Fidelity DNA polymerase

The most important and recommended applications where the AQ97 can be used are in GC-rich DNA templates, long range PCR, sequencing, cloning, mutagenesis, SNP analysis, NGS applications

Features 

  • High fidelity: >60x Taq Fidelity
  • High elongation rate: 10 sec/kb
  • 3’→5’ proofreading exonuclease activity
  • Long range capacity: 18 kb for gDNA and 25 kb for λ DNA
  • Good coverage on DNA templates with low to high GC content

 

Fidelity 

The fidelity of a polymerase refers to its ability to insert the correct base during PCR, and it depends on the polymerase, the buffer system in use and the quality of your template DNA.

The Aq97 polymerase showed error rates below the detection limits, showing its high fidelity skill to achieve less error results. 

For the discussion of more information 

Contact us

AccuPOL DNA polymerase

Our AccuPol is recommended for applications which require extremely high fidelity or blunt ends.  The polymerase is very suitable for mutation studies, cloning, gene expression and construction of libraries.

Features 

  • High fidelity enzyme
  • Proof reading
  • Error rate 1.1 x 10-6
  • Processes up to 3 kb
  • Renders blunt-ended DNA

 

Our polymerase AccuPOL was tested targeting DNA with different lenght and GC content. Regarding the variation lenght, it ranged from 200 bp to above 3.000 bp, whereas the GC content ranged from 30 – 65 %. It showed specific and clear results in short and long lenght DNA targets. 

 

For the discussion of more information 

Contact us

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Contact information

+31 30 6880771
support@isogen-lifescience.com

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ISOgen office
Isogen Lifescience B.V.

Veldzigt 2A
3454 PW De Meern

Contact us

+31 30 6880771
info@isogen-lifescience.com

 

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