RealQ Plus PCR Master Mix
RealQ Plus PCR Master Mixes are ready-to-use 2x master mixes used for different Real-Time PCR applications. Real-Time PCR is a very nice technique to analyse gene expression levels and quantification of DNA.
RealQ Plus master mixes consist of TEMPase Hot Start DNA Polymerase, dNTPs, MgCl2 and a special buffer system necessary to carry out high quality DNA amplification. The RealQ Plus PCR Master Mixes have a high stability and efficiency when working with cDNA and gDNA.
To ensure you that RealQ Plus PCR Master Mix gives the same robust results time after time, we can proudly say that the Master Mix is produced under ISO 9001:2015 conditions.
Two variants are available in combination with three different ROX™ reference dye levels.
RealQ Plus Master Mix Green; DNA-binding fluorescent dye detection
RealQ Plus Master Mix for Probe; probe-base real-time PCR based detection
To select the correct ROX™ reference dye levels for your specific Real-Time PCR applications, check the “Real-Time instrument guide”.
Both Master Mixes can be chosen when many genes need to be analysed within a short amount of time.
• High specificity, reproducibility, efficiency and stability
• Reaction set-up at room temperature
• All-in-one premixed 2x solution
Ask your questions or request a quote.
Amplification plot shows a 4-fold dilution series for the Pthr gene (75 bp); 80 ng to 80 pg of gDNA. RealQ Plus 2x Master Mix for Probe, High ROX™ was used. Samples are in triplicates. Results show a high efficacy and high precision which is close to 100%.
This amplification plot shows high reproducibility capacity of the RealQ Plus 2x Master Mix Green, High ROX and 20 ng gDNA.
Only 0.084 standard deviation after 80 replication