The Small RNA-Seq Library Prep Kit for Illumina consists of all the reagents and components required to generate small RNA libraries to be used for Next Generation Sequencing on an Illumina platform. All molecular reagents including adapters, primers, enzyme mixes, and buffers are provided. A column-based purification is included for rapid purification of nucleic acid products generated during the workflow.
The Small RNA-Seq Library Prep Kit provides a protocol for generating small RNA libraries for Illumina sequencing, directly from total RNA or enriched small RNA. It enables discovery and profiling of all common types of small RNA’s (≤ 200nt), including miRNA, piRNA, siRNA, snoRNA, tsRNA, srRNA and U-RNA.
Benefits of small RNA-Seq Library Prep Kit for Illumina:
The protocol allows for detection of a higher number of microRNA’s compared to other workflows. The difference is especially considerable for lower RNA inputs.
The high sensitivity of the kit makes it the best choice for challenging, low content RNA sources, such as liquid biopsies (plasma, serum and urine), including exosomes. The protocol has also been tested on RNA from cells and tissues and can be used for inputs from 1ng-1µg of cellular total RNA or 50pg-1µg of enriched small RNA, including liquid biopsies.
The protocol exhibits exceptional reproducibility and correlation across samples with different concentrations.
The Small RNA-Seq Library Prep Kit for Illumina offers a time saving protocol that can be completed within 5 hours and requires just 1h15’ of hands-on time. The final library does not need to go through gel purification. Quick and convenient magnetic bead-based purification can optionally be performed to remove adapter dimers for demanding RNA inputs.
With the 96 i7 indices, standardly included in the kit, multiplexing of up to 96 samples is possible. This allows to pool more samples per sequencing lane and to perform cost-efficient experiments on the platforms of different scale.
In order to get high quality input material for the Small RNA Library Prep, we recommend to extract the RNA with the SPLIT RNA extraction kit. This kit enables extracting NGS-grade total RNA or small and large RNA fractions from the same sample. SPLIT is highly suitable for enrichment of small RNA for use with the Small RNA-Seq Library Prep Kit.
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