Exceptional Elimination of cDNA from Degraded RNA
The ligation of the 5’ linker to the end of the 3’ end of the cDNA is based on the Cap-Dependent Linker Ligation (CDLL) technology and happens in a highly cap-dependent manner. This provides an exceptional cap-specificity and eliminates cDNA products from degraded mRNA.
Full-Length cDNA synthesis
cDNA synthesis is based on the unique CDLL and Long reverse transcription (Long RT) technology from Lexogen. This makes TeloPrime highly selective for full-length RNA molecules that are both capped and polyadenylated.
Multiple Downstream Applications
The via TeloPrime generated full-length cDNAs can be used for a great variety of downstream applications (eg. NGS, RACE, cloning, microarray probes and normalization). This kit enables detection and correct quantifications of splice variants and their true transcription start- and end-sites, in both short and long mRNA molecules. For further full-length or gene-specific PCR’s, a TeloPrime PCR Add-on Kit v2 is offered, containing the PCR forward and reverse primer separately, enabling substitution with the gene-specific primer of interest.