DNA/RNA and Protein Sample Preparation

DNA/RNA and Protein Sample Preparation

Isogen Life Science supplies DNA/RNA sample preparation kits by Invitek Molecular and QRET Technologies.
Invitek Molecular delivers a most advanced and comprehensive range of DNA and RNA extraction kits for manually and automated DNA/RNA sample preparation. From sample collection, stabilisation, isolation to purification.
QRET Technologies supplies a complete solution for nucleic acid and protein extraction by providing QuicPick™ magnetic tools and MagRo™ robotic workstation together with QuickPick™ reagents and consumables

Smarter DNA/RNA preparation kits by INVITEK

 

The Invitek sample preparation kits are outstanding because of their advanced production process. The quality of the products is exceedingly high, resulting in the following advantages for you:

  1. Save time with shorter RNA and DNA purification protocols
  2. Higher DNA yield by longer enzymatic activity for sample lysis
  3. More intact DNA – less chemical degradation

Have a look at our Applications!

Sample collection and preservation of Saliva, Swabs, and Stool 

It is important to guarantee high-quality samples for getting excellent results. Our non-invasible collection tubes for DNA Samples:

  • Cost saving: avoid cooling, eliminate costs associated with shipping and temperature control. 
  • Easy and intuitive use
  • Reliable results: Minimise bias by microbial growth 

Our sample collection and stabiliser kits enables long-term stability of DNA in stool samples (up to 105 days), in saliva samples (up to 1 year). 

For more information you can have a look at the brochure or contact us!

 

 

 

 

 

INVISORB - Spin Soil DNA kit 

Save time with our kit because of its short protocol. The kit enables DNA isolation from microorganisms in soil samples. The technology used is spin column technology. 

For more information, you can have a look at the brochure. 

DNA Clean Up 

Our DNA Clean-up is an easy-to-use kit for routine molecular biology applications. 

  • Save time: 7 min protocol for cleaning up PCR and enzymatic reactions (MSB Spin PCRapace) and 20 mins for DNA fragment clean-up from agarose gels (Invisorb Fragment Clean-up)
  • 95% recovery of fragment sizes between 80 bp and 30 kb.

We do also offer sample preparation for infectious diseasesnucleic acid extraction kits for infectious diseases. 

Applications

  • Purification of microbial DNA by combining Stool Collection tubes with DNA stabiliser kits: Read the application note here for combination with Zymo kits, and here for Qiagen kits. 
  • Is freezing necessary for conservation of microbiome diversity in stool samples? Have a look at the appnote here to get to know the answer. 

 

Do not hesitate to contact us to find the kit that better matches your needs!

Contact us

InviMag technology

Magnetic beads for DNA/RNA sample preparation - Preparation on KingFisher Systems 

 

The InviMag magnetic beads are developed and selected for optimal performance in a variety of applications. Based on the application, sample characteristics and instrument platform used, Invitek Molecular selects the InviMag beads best suited for each particular purpose. 

  • Fast magnetic velocity via high magnetic content (70–90 % of iron oxides)
  • A variety of binding capacities through different-sized beads (200 nm–1 µm)
  • Excellent reproducibility and stability through uniform size distribution
  • Superior suspension stability
  • Application fields: Cancer genetics, Genotyping, Plant genotyping, GMO analysis, Pathogen Detetion, Gene Expression Analysis and Cloning experiments. 

Benefits

  • Fully automated processing on InviGenius PLUS starting from DNA
  • Standardised walk-away workflow starting from blood, FFPE or plasma sample to bisulfite conversion of the isolated DNA on the instrument possible
  • Only 10 min hands-on time

For example The InviMag® Bisulfite Conversion

The InviMag® Bisulfite Conversion Kit/ IG features a fully automated and reliable bisulfite treatment and conversion of cfDNA and genomic DNA from whole blood, cell cultures, tissue samples and FFPE slides for methylation analysis. The process is fully automated on the InviGenius® PLUS and can be combined with previous protein, DNA and RNA extraction workflows on the instrument.

RTP Technology – Ready to prep

The RTP technology is based on the patented non-chaotropic chemistry Invitek is using. With this technology nucleic acids are able to bind in very low salt concentrations which reduces the amount of wash steps in the DNA and RNA isolation process and thereby leading to faster protocols.  

MSB Technology – Minimal salt binding 

MSB products will purify DNA from reaction mixtures like PCR, cDNA synthesis, restriction enzyme digests or other enzymatic reactions. DNA fragments from 80 bp to 30 kbp are recovered with very high yields in a final elution volume as low as 10 µl.

Range of products

A full range of priotein, DNA and RNA purification products and formats, more than 120, for your DNA/RNA application demands. A wide selection of products for high-throughput and automated sample processing. Many products are developed and manufactured in compliance with the Directive 98/79/EC on in vitro diagnostic medical devices (IVD-Directive).


Want to know which DNA/RNA sample preparation kit you need?

Download the product selection guide or contact our office, and we will make sure you get the right information and product selection guide.

 

DNA/RNA and Protein purification by QRET Technologies

 

Purification solutions                                     

By moving particles, not liquids, the QuickPick™ method offers a clear advantage over conventional protein, RNA and DNA isolation methods. Using the QuickPick method, your nucleic acid and protein purification method is faster, simpler and gentle from different sample sources: plants, human tissue, animal tissue, etc. 

Magnetic DNA and RNA purification

Magnetic protein, DNA and RNA extraction is built on the affinity on the surface of magnetic particles. A suspension of magnetic particles is mixed with a preparation of the target molecule. Once the target is bound to the group, a magnet is used to separate the target-bound particles from the solution. The unbound material can then be washed away from the particles. Depending on the application, the target molecules can, if necessary, be separated from the magnetic particles.

Magnetic particle technology has several advantages

  • Fast and simple
  • More economical as other separation methods as centrifugation and vacuum DNA, RNA and protein extraction and softer on the sample. 
  • Less contamination because of moving particles instead of liquids. 

QuicPick™ tools

  • QuicPick™ 1-magnet tool 
  • QuicPick™ multiEight 8-magnet tool 
  • MagRo 96-M workstation

Want to know more about the QuicPick™ tools, please contact us to discover more about it!

QRET Technologies provides a range of QuickPick reagents for nucleic acid and protein purification and besides that a wide range of coated magnetic particles for your own applications.

DNA and RNA purification 

QuickPick Plant DNA and QuickPick DNA reagents are optimised for a range of sample materials and volumes. You can use the same reagents with PickPen manual tools and with the MagRo™ 96-M robotic workstation for high-quality purified DNA.

QuickPick DNA Fragment purification kit provides:

You can use the same reagents with QuicPick manual tools and with the MagRo 96-M robotic workstation for high-quality mRNA.

Benefits of QuickPick™ RNA and DNA purifications

  • Flexible: wide range of sample materials
  • Scalable: easy solution for variable sample volumes
  • Fast DNA and RNA extraction: 96 samples in one hour, with rapid protocols that ensure high-quality products.
  • Short hands-on-time
  • Move particles, not liquids, to avoid time-consuming aspirating and dispensing steps, just like minimising contamination.
  • Reliable: Reproducible results, robust protocols and high-quality purified product

Range of the QuickPick™ protein, DNA and RNA extraction kits for

  • Plant DNA
  • Genomic DNA
  • DNA fragment
  • Plasmid DNA
  • Total RNA
  • Messenger RNA

Contact us for discussing your research field and which acid nucleic purification kit could match better your needs! 

 

Protein purification

QuickPick IMAC and IMAC Plus extraction kits are used for his-tag protein isolation and QuickPick GST kit provides an easy alternative for purification of fusion protein.

For simple fractionation of complex protein samples we offer QuickPick DEAE and CM extraction kits based on ion-exchange magnetic particles.
 
For more information on specific applications, please contact us for available brochures, application notes and technical notes on a specific application.

Plant DNA & RNA Purification Kit by Canvax Biotech

DNA & RNA Extraction Kits

CanvaxExtraction Kits are designed for a Reliable, Easy & Fast Purification of High-Quality/High-Purity Genomic DNA/RNA from 25+ different starting materials, like Blood, Buccal Swab, Buccal Saliva, Tissues, Cultured Cells, Stool samples, Plant tissues, Soil samples, Bacteria, Yeast, Plasmid, PCR mixtures, Agarose gel slices or Serum.

 

HigherPurity™ Plant DNA and RNA Purification Kit
Purification of Total DNA from a wide variety of Plant Tissues in MiniSpin Column Format. The kit uses HigherPurity™ optimized technology based in DNA ability and RNA ability to bind silica in the presence of high concentrations of chaotropic salts.

  • Highly Efficient: average yields up to 5-40 μg of Total DNA.
  • Pure genomic DNA: ready-to-use in all Molecular Biology applications like RT-PCR, Southern Blotting or RFLP for DNA samples, and RT-PCR, and Northern Blotting for RNA samples. 
  • Save time: 56 minutes protocol to get the results with minimal handling steps, even from difficult samples for DNA purification, while for RNA it takes only 28 minutes. 
  • High-quality DNA obtained from a wide types of plants.
  • Convenient and save costs: includes all necessary components for DNA Purification.

For more information you can have a look at the brochure here!  

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support@isogen-lifescience.com

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