Biodiversity and NextGenPCR

ZPRIZE Rainforest 2023 Collaboration

Company: Museum für Naturkunde Berlin and PROTOLABS Belgium.

Project needs: study biodiversity in 100 hectares of tropical rainforest in 24 hours with the most impactful real-time insights.

Our solution: Our NextGenPCR instrument reduces DNA amplification time for end-point applications dramatically. Saving time in the PCR steps helps you to reduce the time of the whole sequence workflow. Therefore, more samples can be analyse a day increasing samples throughput.

Results: 313bp barcode was amplified in just 22 minutes.  

Rewarding Sustainable Forest Management practices will enhance biodiversity  and climate resilience of EU forests - Papnews

ZPRIZE Rainforest 2023 Collaboration

In the last 45 years, forest vertebrate populations have declined by 50% and every time more species are in extinction. The XPRIZE Rainforest competition promotes the development of innovative approaches to demonstrate the capability and readiness of their technology in a rainforest setting.

The main goal is to study biodiversity in 100 hectares of tropical rainforest in 24 hours and produce the most impactful real-time insights within 48 hours to win the competition.

Isogen Life Science participated together with MBS (NextGenPCR technology creator) and the Belgian company PROTOLABS and its XiniX AI, which creates an all-terrain robot to explore tropical rainforests.

Our Solution NextGen PCR

Our NextGenPCR solution is part of the workflow implemented in the competition experiments.

  • 24h sampling: Set up of all the instruments and genomics tent to do in field expedition.
  • Front-mounted cameras to pilot robots to caught insects.
  • 48h Data Analysis at the National University of Singapore
    • Sample recognition preparation
    • DNA extraction
    • PCR Amplification with our NextGenPCR System following a protocol
    • Sequencing fly poop with the MiniION

Key findings: 313bp barcode amplified in just 22 minutes!

Flies were captured with the robots in the field and were analyzed later. The analysis was performed by sequencing fly poop, and therefore gut content samples.

The protocol was based on the workflow that the Museum für Naturkunde in Berlin performed. The PCR amplification step for the COI fragment took only 22 minutes using the NextGenPCR thermocycle. Therefore, it was possible to barcode 250 fly specimens in just 6 hours, which would have taken significantly longer using the traditional method. Moreover, it uses 80x less energy.

RESULTS FROM THE SPECIES SEQUENXCES BY USEING GUT POOP FLIES

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