RNA Enrichment and Depletion - Lexogen

When looking at total RNA, about 80% is ribosomal RNA (rRNA) and is mostly unwanted in RNA-seq experiments. When rRNA is not removed from the sample during library prep, it will occupy most of the sequencing space, reducing the number of samples that can be pooled on a single lane of a flow cell and therefore, increase the sequencing cost dramatically.

Next to total RNA-seq, mRNA-seq is also an important application. Knowing that the mRNA-fraction only represents a few percentages of the total RNA, it is good practice to enrich for this fraction prior to mRNA library prep by selecting and capturing the poly-A tailed mRNA.

We do offer 2 different options for rRNA depletion or Poly-A selection. Combined with the CORALL total RNA-seq library prep kit, this represents a powerful and streamlined method for total RNA- and mRNA-seq

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RiboCop rRNA Depletion Kit V1.2 (H/M/R)

About 80% of the total RNA after extraction is ribosomal RNA (rRNA) which is generally of little interest. The RiboCop rRNA depletion kit enables removal of rRNA from total RNA and is suited for NGS as well as other demanding RNA Analysis applications

  • Efficient elimination of cytoplasmic and mitochondrial rRNA
  • Simple workflow; no enzymatic reactions
  • Output ready for NGS Library Prep
  • Down to 1ng of total RNA input
  • Fast protocol: rRNA depleted in just 1,5 hours

Efficient Elimination of rRNA

RiboCop enables efficient removal of cytoplasmic 28S, 18S, 5.8S, 45S, 5S, as well as mitochondrial mt16S, mt12S ribosomal RNA sequences in human, mouse and rat samples. Sequencing depth is preserved for relevant RNA species.


Flexible Input Requirements

RiboCop is suitable for rRNA depletion of 1ng - 1µg total RNA input amounts from intact or degraded RNA, including FFPE samples

RiboCop rRNA Depletion Kit

Simple Workflow

The RiboCop protocol allows rRNA depletion on only 1.5 hours, including a hands-on time of 30 minutes. It does not involve enzymatic reactions or mechanical shearing steps, leaving full-length transcripts intact for downstream processing.

Automation friendly

Magnetic beads are used for depletion and purification steps, rendering the protocol completely automation friendly.

Ordering Information:

Cat.no: 037.24 - Description: RiboCop rRNA Depletion Kit (H/M/R) v1.2 - Reaction size: 24 preps 

Cat.no: 037.96 - Description: RiboCop rRNA Depletion Kit (H/M/R) v1.2 - Reaction size: 96 preps 

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RiboCop rRNA Depletion Kits Bacteria 

Coming soon. 

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Poly-A RNA Selection Kit

The Poly-A RNA Selection Kit is the ideal solution for rapid and highly specific enrichment of polyadenylated RNAs from total RNA samples.

  • Highly specific poly-A enrichment
  • Magnetic bead-based purification
  • Fast workflow

Highly specific for poly-A RNA

Other RNA species (rRNA and tRNA) do not contain poly-A sequences and will not bind to the oligo(dT) beads.

Rapid Turnaround

The Poly-A RNA selection kit enables isolation of poly-A RNA from total RNA in about 1 hour.

Various downstream Applications

The enriched mRNA fraction can directly be used for RNA-seq library prep, SAGE, CAGE, cloning, micro-arrays, cDNA synthesis etc.

Ordering Information:

Cat.no: 039.100 - Description: Poly-A RNA Selection Kit - Reaction size: 100 preps 

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Poly-A RNA Selection Kit
figure 1: Bioanalyzer traces of the poly-A RNA enrichment using 5µg of Universal Human Reference RNA (UHRR) (A) input with the poly-A RNA selection kit. Recovery of poly-A selected UHRR (B) is about 2% of the initial input amount.

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support@isogen-lifescience.com

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