RNA Enrichment and Depletion - Lexogen

Name: Isogen Life Science B.V.
Address: Veldzigt 2a, De Meern, 3454 PW, NL
Telephone: +31 30 6880771

When looking at total RNA, about 80% is ribosomal RNA (rRNA) and is mostly unwanted in RNA-seq experiments. When rRNA is not removed from the sample during library prep, it will occupy most of the sequencing space, reducing the number of samples that can be pooled on a single lane of a flow cell and therefore, increase the sequencing cost dramatically.

Next to total RNA-seq, mRNA-seq is also an important application. Knowing that the mRNA-fraction only represents a few percentages of the total RNA, it is good practice to enrich for this fraction prior to mRNA library prep by selecting and capturing the poly-A tailed mRNA.

We do offer 2 different options for rRNA depletion or Poly-A selection. Combined with the CORALL total RNA-seq library prep kit, this represents a powerful and streamlined method for total RNA- and mRNA-seq

RiboCop rRNA Depletion Kit V.2 (Human/Mouse/Rat)
RiboCop rRNA Depletion Kits for Bacteria
RiboCop rRNA Depletion Kit for Yeast NEW!!
Poly-A RNA Selection Kit

Product information

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RiboCop rRNA Depletion Kit V.2 (H/M/R)

About 80% of the total RNA after extraction is ribosomal RNA (rRNA) which is generally of little interest. The RiboCop rRNA depletion kit enables removal of rRNA from total RNA and is suited for NGS as well as other demanding RNA Analysis applications

Efficient elimination of cytoplasmic and mitochondrial rRNA
Simple workflow; no enzymatic reactions
Output ready for NGS Library Prep
Down to 1ng of total RNA input
Fast protocol: rRNA depleted in just 1,5 hours
HMR with Globin for blood RNA samples

Efficient Elimination of rRNA

RiboCop enables efficient removal of cytoplasmic 28S, 18S, 5.8S, 45S, 5S, as well as mitochondrial mt16S, mt12S ribosomal RNA sequences in human, mouse and rat samples. Sequencing depth is preserved for relevant RNA species.

Flexible Input Requirements

RiboCop is suitable for rRNA depletion of 1ng - 1µg total RNA input amounts from intact or degraded RNA, including FFPE samples

Simple Workflow

The RiboCop protocol allows rRNA depletion on only 1.5 hours, including a hands-on time of 30 minutes. It does not involve enzymatic reactions or mechanical shearing steps, leaving full-length transcripts intact for downstream processing.

Automation friendly

Magnetic beads are used for depletion and purification steps, rendering the protocol completely automation friendly.

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RiboCop rRNA Depletion Kits Bacteria

RiboCop bacteria is ideal for RNA depletion of bacteria. Bacterial RNA consists 98% of ribosomal RNA, which can make it challenging to analyse the transcriptome capacity of complex cultures. With the RiboCop rRNA depletion kit, 23S, 16S and 5S rRNA is effectively removed from mixed or monocultures.

With just 1ng to 1ug total RNA input, the rRNA depletion kit reduces rRNA to 1-3%, while maintaining unbiased transcript abundance.

The robust protocols, make it highly reproducible. Depletions without off-target effects are performed.

The RiboCop rRNA depletion kit is a stand-alone kit with the option to choose from three optimised Probe Mixes for depletion of rRNA from mixed bacterial samples (META) or from Gram negative or Gram-positive bacteria (G- or G+) grown in monoculture.

Benefits of the RiboCop rRNA depletion kit for bacteria

Ideal for complex, mixed and monocultures
Reduces rRNA to 1-3%
Unbiased transcript abundance

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RiboCop rRNA Depletion Kit for Yeast

Yeast total RNA is comprised of large amounts of undesired ribosomal RNA (rRNA) which accounts for up to ~97 % of all transcripts. Our RiboCop rRNA Depletion Kits for Yeast remove undesired cytoplasmic (25S, 18S, 5.8S, 5S, and 35S) and mitochondrial (21S, 15S, ACI60_gr01 and ACI60_gr02) rRNA from intact as well as degraded input RNA. Ribo-depletion affords an unbiased view of the transcriptome, including non-coding and non-polyadenylated RNA species.

Reduces rRNA reads to 1%
Maintains unbasied Expression Profiles
Increases Gene detection upon rRNA Depletion

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Poly-A RNA Selection Kit

The Poly-A RNA Selection Kit is the ideal solution for rapid and highly specific enrichment of polyadenylated RNAs from total RNA samples.

Highly specific poly-A enrichment
Magnetic bead-based purification
Fast workflow

Highly specific for poly-A RNA

Other RNA species (rRNA and tRNA) do not contain poly-A sequences and will not bind to the oligo(dT) beads.

Rapid Turnaround

The Poly-A RNA selection kit enables isolation of poly-A RNA from total RNA in about 1 hour.

Various downstream Applications

The enriched mRNA fraction can directly be used for RNA-seq library prep, SAGE, CAGE, cloning, micro-arrays, cDNA synthesis etc.

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Why Isogen Life Science?

At Isogen Life Science, we believe that long term relationships with life science professionals and suppliers are fundamental to solve life science problems. Our curiosity drives us to continually stay involved and to dig deep in dialogue, with both clients and suppliers. It’s these intensive collaborations that enable us to develop tailored, innovative products that push life science’s boundaries. Partner up with us and enjoy our quick support for troubleshooting and questions!