The PHERAstar is optimal for High-Throughput Screening as it has a high sensitivity and speed. This multimode plate reader can analyse wells with UV-Vis absorption, fluorescence (TR-FRET, FRET, polarised), luminescence (BRET) and AlphaScreen/LISA/Plex. This wide range of analysis possibilities is enabled by the three light sources: high energy xenon flashlamp, laser for TRF/TR-FRET and a laser for AlphaScreen/LISA/Plex.
The PHERAstar obtains the technique for simultaneous dual emission (SDE), this reduces the reading time. In simultaneous dual emission assays the variability caused by changes in pH, temperature and evaporation can also be reduced. It also eliminates the drawbacks of double sequential detection. Additionally, a decay curve monitoring for TR-FRET is present, which can be used for the development of assays or assay fine-tuning. It visualises and measures the time resolved emission curve of a fluorophore.