PCR Kits

Name: Isogen Life Science B.V.
Address: Veldzigt 2a, De Meern, 3454 PW, NL
Telephone: +31 30 6880771

PCR kits

RT-PCR kit for SARS Co-V-2
PCR kit for Monkeypox

RT-PCR kit for SARS-CoV-2

The PCR kit consist of a RT-PCR Chemistry-2x mixture, nuclease free water, primers and probes and a positive control.

This kit is optimised for the NGPCR, creating even faster results.

This kit contains enough for 100 reactions.

RT-PCR Chemistry-2x

The RT-PCR Chemistry-2x for the RT-PCR consist of a mixture of reverse transcriptase, DNA polymerase, dNTP's and oligonucleotides under buffered conditions. Additionally, a bottle of nuclease free water is provided.

The PCR kit generates the first cDNA strand and amplifies DNA in a single PCR reaction. The nuclease free water may be used to dilute the RT-PCR Chemistry-2x mixture.

SARS-CoV-2 and Human RNase P Primers and Probes

The SARS-CoV-2 and Human RNase P Primers and Probes consist of a mixture of labelled and unlabelled oligonucleotides under buffered conditions. With a total of 3 sets of primers with corresponding probes. Two sets target the Orf1ab and N-gene of the SARS-CoV-2 genome and the last set targets the human RNase P gene. Both probes that hybridise to SARS-CoV-2 sequences are labelled with fluorescein amidites (FAM). The probe that hybridises to RNase P sequence is labelled with Cy5.

Download the Application Note

Positive Control- 2019-nCoV N & hRPP30

This positive control is a mixture of control plasmids that contain the complete nucleocapsid gene from 2019-nCoV and a portion of the human RPP30 gene, a single copy gene in the human genome. Specifically, the nucleocapsid gen sequence is from severe acute respiratory syndrome coronavirus 2 isolate Wuhan-Hu-1, complete genome. Delivered at 200 copies/µL in TE pH 7.5.

Click here for more extensive quality controls of Qnostics for over the whole process for SARS-CoV-2 and other infectious diseases.

PCR kit for Monkeypox

To aid laboratories with their Monkeypox virus (MPXV) surveillance effort, this library prep kit contains pre-mixed Monkeypox sequencing primer pools (for a total of 500 samples) and the NextGenPCR® Arctic Fox HF Chemistry2x (ready to use mastermix). Amplification of the 190 kb MPXV genome was achieved by using a PCR-based amplicon tiling of ~2.5kb segments (88 amplicons total). With NextGenPCR® ultra-fast PCR chemistry, the complete PCR cycling program of the protocol can be completed in less than 45 minutes by using the NextGenPCR® thermocycler.

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The composition of the oligonucleotide primer panel was developed by Martin Schou Pedersen from the Department of Clinical Microbiology at Rigshospitalet, Copenhagen University Hospital, Denmark and the NextGenPCR® MPXV library preparation protocol was tested on 40 samples in close collaboration with Matthijs Welkers and Marcel Jonges from the Dept. of Medical Microbiology & Infection Prevention at Amsterdam UMC (location AMC), The Netherlands.

Download the application note

Download the SARS-CoV validation article

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Why Isogen Life Science?

At Isogen Life Science, we believe that long term relationships with life science professionals and suppliers are fundamental to solve life science problems. Our curiosity drives us to continually stay involved and to dig deep in dialogue, with both clients and suppliers. It’s these intensive collaborations that enable us to develop tailored, innovative products that push life science’s boundaries. Partner up with us and enjoy our quick support for troubleshooting and questions!