The PCR kit consist of a RT-PCR Chemistry-2x mixture, nuclease free water, primers and probes and a positive control.
This kit is optimised for the NGPCR, creating even faster results.
This kit contains enough for 100 reactions.
The RT-PCR Chemistry-2x for the RT-PCR consist of a mixture of reverse transcriptase, DNA polymerase, dNTP's and oligonucleotides under buffered conditions. Additionally, a bottle of nuclease free water is provided.
The PCR kit generates the first cDNA strand and amplifies DNA in a single PCR reaction. The nuclease free water may be used to dilute the RT-PCR Chemistry-2x mixture.
To aid laboratories with their Monkeypox virus (MPXV) surveillance effort, this library prep kit contains pre-mixed Monkeypox sequencing primer pools (for a total of 500 samples) and the NextGenPCR® Arctic Fox HF Chemistry2x (ready to use mastermix). Amplification of the 190 kb MPXV genome was achieved by using a PCR-based amplicon tiling of ~2.5kb segments (88 amplicons total). With NextGenPCR® ultra-fast PCR chemistry, the complete PCR cycling program of the protocol can be completed in less than 45 minutes by using the NextGenPCR® thermocycler.