The PCR kit consist of a RT-PCR Chemistry-2x mixture, nuclease free water, primers and probes and a positive control.
This kit is optimised for the NGPCR, creating even faster results.
This kit contains enough for 100 reactions.
The RT-PCR Chemistry-2x for the RT-PCR consist of a mixture of reverse transcriptase, DNA polymerase, dNTP's and oligonucleotides under buffered conditions. Additionally, a bottle of nuclease free water is provided.
The PCR kit generates the first cDNA strand and amplifies DNA in a single PCR reaction. The nuclease free water may be used to dilute the RT-PCR Chemistry-2x mixture.
The SARS-CoV-2 and Human RNase P Primers and Probes consist of a mixture of labelled and unlabelled oligonucleotides under buffered conditions. With a total of 3 sets of primers with corresponding probes. Two sets target the Orf1ab and N-gene of the SARS-CoV-2 genome and the last set targets the human RNase P gene. Both probes that hybridise to SARS-CoV-2 sequences are labelled with fluorescein amidites (FAM). The probe that hybridises to RNase P sequence is labelled with Cy5.
This positive control is a mixture of control plasmids that contain the complete nucleocapsid gene from 2019-nCoV and a portion of the human RPP30 gene, a single copy gene in the human genome. Specifically, the nucleocapsid gen sequence is from severe acute respiratory syndrome coronavirus 2 isolate Wuhan-Hu-1, complete genome. Delivered at 200 copies/µL in TE pH 7.5.
To aid laboratories with their Monkeypox virus (MPXV) surveillance effort, this library prep kit contains pre-mixed Monkeypox sequencing primer pools (for a total of 500 samples) and the NextGenPCR® Arctic Fox HF Chemistry2x (ready to use mastermix). Amplification of the 190 kb MPXV genome was achieved by using a PCR-based amplicon tiling of ~2.5kb segments (88 amplicons total). With NextGenPCR® ultra-fast PCR chemistry, the complete PCR cycling program of the protocol can be completed in less than 45 minutes by using the NextGenPCR® thermocycler.
The composition of the oligonucleotide primer panel was developed by Martin Schou Pedersen from the Department of Clinical Microbiology at Rigshospitalet, Copenhagen University Hospital, Denmark and the NextGenPCR® MPXV library preparation protocol was tested on 40 samples in close collaboration with Matthijs Welkers and Marcel Jonges from the Dept. of Medical Microbiology & Infection Prevention at Amsterdam UMC (location AMC), The Netherlands.
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