The G2 DNA/RNA Enhancer is a tool to help and increase the microbial DNA yield during DNA extraction from difficult matrices like soil and clay samples. The main function of G2 DNA/RNA Enhancer is to eliminate inhibitory DNA particles or other inhibitory DNA complexes.
The G2 DNA/RNA Enhancer is available in two ways; freeze dried with 0.1 mm or 1.4 mm beads in a 2 ml tube or in liquid form in three packing sizes (10, 50 or 100 reactions of 500 µl).
The G2 DNA/RNA Enhancer should be used either with standardised extraction protocols or commercially available DNA/RNA extraction kits.
DNA yield from soil is significantly increased by G2 DNA/RNA enhancer
8 different laboratories have tested the same 25 low biomass clay sub-soil samples with and without G2 DNA/RNA Enhancer beads. All in combination with a commercial kit for DNA extraction from the soil. The DNA yield with G2 DNA/RNA Enhancer has an increase up to 10-fold compared to the untreated samples. All samples were analysed for the quantity of rpoB gene copies. Significance of the triplicates DNA extracts was tested using T-test.
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