The PCR Buffer system
Multiple factors play an important role to gain reliable PCR results. Besides the DNA quality and the PCR instrument itself, choosing the right buffer is essential for creating an optimal environment when performing PCRs.
To match with your desired PCR assay conditions and applications, several Tris-based solutions are available in different compositions, such as with and without Mg2+, Tween 20 or Triton X-100. This way, it makes it easy to choose the right combination of DNA Polymerase/buffer or Master Mix for your personalized PCR reaction.
We recommend to use ammonium buffer for most PCR reactions. It favours high yield, steady amplification and also high specificity. For an overview of all of our buffers, download our brochure.
TIP – Choose the right buffer
We recommend to use ammonium buffer for most PCR applications. High yield can be expected and minimal optimisation is needed regarding annealing temperatures or Mg2+ concentrations.
We recommend using Mg2+ free buffer if you need to optimize your PCR protocol, especially if Mg2+ concentrations lower than 1.5 mM are required in your set-up.
We recommend using detergent free buffers if fluorescent spectrometry is involved in automation and downstream applications.