Buffers

Isogen LifescienceProductsReagents and KitsPCR ReagentsBuffers

Multiple factors play an important role to gain reliable PCR results. Besides the DNA quality and the PCR instrument itself, choosing the right buffer is essential for creating an optimal environment when performing PCRs.

To match with your desired PCR assay conditions and applications, several Tris-based solutions are available in different compositions, such as with and without Mg2+, Tween 20 or Triton X-100. This way, it makes it easy to choose the right combination of DNA Polymerase/buffer or Master Mix for your personalized PCR reaction.

  • Ammonium Buffer
  • Standard Buffer 
  • Combination Buffer
  • Other buffers 

 

We recommend to use ammonium buffer for most PCR reactions. It favours high yield, steady amplification and also high specificity. For an overview of all of our buffers, download our brochure.

 

Ammonium Buffer

We recommend to use ammonium buffer for most PCR reactions. Besides that it favours a high PCR yield, steady amplification and comes with high specificity, it minimises the need for optimization of annealing temperatures and/or Mg2+ concentrations. Ammonium buffer also works well when working with difficult templates, such as GC-rich DNA sequences.

Features

  • Promotes amplification on most DNA templates
  • High yield & specificity Picture: “Ampliqon 10x Ammonium Buffer, 15 mM”
  • Tolerance for primer annealing temperatures
  • 10X buffer

Available in 4 options:

  • 15 mM MgCl2
  • Mg2+ free
  • Tween free
  • Mg2+ & Tween free

 

Ask your questions or request a quote.

Ammonium Buffer: Minimal need for optimisation.

A broad range of Mg2+ concentrations and temperatures results in specific products with high yield (lanes 1.5 – 2.5 and lanes 57 – 66).

PCR amplifications of ENG9. Upper buffer was used in combination with TEMPase polymerase at indicated Mg2+ concentrations or temperatures. In the left part, a Mg2+ dilution series is shown from 0.5 – 4.5 mM Mg2+ at 60°C. The right part shows a temperature gradient from 51 – 66°C at 1.5 mM Mg2+. M: marker.

 

TIP – CHOOSE THE RIGHT BUFFER

We recommend using Mg2+ free buffer if you need to optimize your PCR protocol, especially if Mg2+ concentrations lower than 1.5 mM are required in your set-up.

Standard Buffer

Although we recommend using ammonium buffer, when you already have optimised your PCR protocols for this buffer, we recommend to keep using the Standard Buffer.

The buffer is based on potassium and has a high specificity. Nevertheless, it usually needs optimisation regarding primer annealing temperatures and Mg2+ concentrations.

DNA templates with a high purity are desired in combination with this buffer.

Features

  • Promotes reliable amplification on most DNA templates Picture: “Ampliqon 10x Standard Buffer 15 mM”.
  • High specificity
  • 10X buffer

Available in 4 options:

  • 15 mM MgCl2
  • Mg2+ free
  • Tween free
  • Mg2+ & Tween free

Standard Buffer: Optimisation is needed.
A narrow range of Mg2+ concentrations and temperatures results in specific products (lanes 1.5 – 2.0 and lanes 60 – 66).

PCR amplifications of ENG9. Upper buffer was used in combination with TEMPase polymerase at indicated Mg2+ concentrations or temperatures. In the left part, a Mg2+ dilution series is shown from 0.5 – 4.5 mM Mg2+ at 60°C. The right part shows a temperature gradient from 51 – 66°C at 1.5 mM Mg2+. M: marker.

TIP – CHOOSE THE RIGHT BUFFER

We recommend to use ammonium buffer for most PCR applications. High yield can be expected and minimal optimisation is needed regarding annealing temperatures or Mg2+ concentrations.

We recommend using Mg2+ free buffer if you need to optimize your PCR protocol, especially if Mg2+ concentrations lower than 1.5 mM are required in your set-up.

We recommend using detergent free buffers if fluorescent spectrometry is involved in automation and downstream applications.

Combination Buffer

Combination Buffer is a balanced formulation ammonium-potassium PCR buffer which results in high product yield and good specificity. This buffer shows good results on several PCR instruments and is worth testing when setting up a new protocol.

Features

  • Promotes reliable amplification on most DNA templates
  • Tolerance toward primer annealing temperature
  • 10X buffer

Available in 4 options:

  • 15 mM MgCl2
  • Mg2+ free
  • Tween free
  • Mg2+ & Tween free

Combination Buffer: Optimisation is needed.

A narrow range of Mg2+ concentrations and temperatures results in specific products with high yields (lane 1.5 and lanes 60 – 66).

PCR amplifications of ENG9. Upper buffer was used in combination with TEMPase polymerase at indicated Mg2+ concentrations or temperatures. In the left part, a Mg2+ dilution series is shown from 0.5 – 4.5 mM Mg2+ at 60°C. The right part shows a temperature gradient from 51 – 66°C at 1.5 mM Mg2+. M: marker.

 

TIP – CHOOSE THE RIGHT BUFFER

We recommend to use ammonium buffer for most PCR applications. High yield can be expected and minimal optimisation is needed regarding annealing temperatures or Mg2+ concentrations.

We recommend using Mg2+ free buffer if you need to optimize your PCR protocol, especially if Mg2+ concentrations lower than 1.5 mM are required in your set-up.

We recommend using detergent free buffers if fluorescent spectrometry is involved in automation and downstream applications.

 

Other buffers

If the previously described buffers do not match to your requirements, we also offer some other buffers that are listed below.

  • 5x PCR Buffer Red

  • 4x GC Buffer l & 4x GC Buffer ll

  • PCR Grade Water

  • Betaine Enhancer Solution 5 M

 

We are happy to help you.

You can contact us by filling in the form below. We will try to answer your question within one working day.

Contact information

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support@isogen-lifescience.com

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