Buffers

The PCR Buffer system

Do you want to get more reliable results? It is known that multiple factors play an important such as the quality of the DNA and the quality of the  instrument itself.  Therefore, choosing the right buffer is essential for creating an optimal environment when performing PCRs.

We offer you several Tris-based solutions that may match your desired PCR assay conditions and applications. The buffers are available in different compositions, and with different components. For instance, we offer buffers with and without Mg²⁺, Tween 20 or Triton X-100. 

• Ammonium Buffer  RECOMMENDED!
• Standard Buffer 
• Combination Buffer
• Other buffers 

All the buffers are available in 4 options: 15 mM MgCl2, Mg2+ free, Tween free and Mg2+ & Tween free

Contact us to know which one matches better your research 

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TIP – Choose the right buffer

The PCR Ammonium buffer includes high yield expectation and a minimal optimisation is needed regarding annealing temperatures or Mg2+ concentrations. For these reasons, we recommend to use ammonium buffer for most PCR applications. Specifically:

• Use Mg2+ free buffer for the optimization of your PCR protocol, especially if Mg2+ concentrations lower than 1.5 mM are required in your set-up.
• Use of detergent free buffers if fluorescent spectrometry is involved in automation and downstream applications.

Standard Buffer

Although we recommend using ammonium buffer, when you already have optimised your PCR protocols for this buffer, we recommend to keep using the Standard Buffer.

The buffer is based on potassium and has a high specificity. Nevertheless, it usually needs optimisation regarding primer annealing temperatures and Mg2+ concentrations.

DNA templates with a high purity are desired in combination with this buffer.

Features

• Promotes reliable amplification on most DNA templates Picture: “Ampliqon 10x Standard Buffer 15 mM”.
• High specificity
• 10X buffer

Standard Buffer: Optimisation is needed.
A narrow range of Mg2+ concentrations and temperatures results in specific products (lanes 1.5 – 2.0 and lanes 60 – 66).

PCR amplifications of ENG9. Upper buffer was used in combination with TEMPase polymerase at indicated Mg2+ concentrations or temperatures. In the left part, a Mg2+ dilution series is shown from 0.5 – 4.5 mM Mg2+ at 60°C. The right part shows a temperature gradient from 51 – 66°C at 1.5 mM Mg2+. M: marker.