Buffers

Name: Isogen Life Science B.V.
Address: Veldzigt 2a, De Meern, 3454 PW, NL
Telephone: +31 30 6880771

PCR Buffers system

Do you want to get more reliable results? It is known that multiple factors play an important such as the quality of the DNA and the quality of the instrument itself. Therefore, choosing the right PCR buffers is essential for creating an optimal environment when performing PCR.

We offer you several Tris-based solutions that may match your desired PCR assay conditions and applications. The PCR buffers are available in different compositions, and with different components. For instance, we offer buffers with and without Mg²⁺, Tween 20 or Triton X-100.

Ammonium Buffer RECOMMENDED!
Standard Buffer
Combination Buffer
Other buffers

All the PCR buffers are available in 4 options: 15 mM MgCl2, Mg2+ free, Tween free and Mg2+ & Tween free

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TIP – Choose the right buffer

The PCR Ammonium buffer includes high yield expectation and a minimal optimisation is needed regarding annealing temperatures or Mg2+ concentrations. For these reasons, from all the PCR buffers we recommend to use ammonium buffer for most PCR applications. Specifically:

Use Mg2+ free buffer for the optimization of your PCR protocol, especially if Mg2+ concentrations lower than 1.5 mM are required in your set-up.
Use of detergent free buffers if fluorescent spectrometry is involved in automation and downstream applications.

Ammonium Buffer

The ammonium buffer favors a high PCR yield, steady amplification and comes with high specificity. It minimizes the need for optimisation of annealing temperatures and/or Mg2+ concentrations. Ammonium buffer also works well when working with difficult templates, such as GC-rich DNA sequences.

Features

Promotes amplification on most DNA templates
High yield & specificity Picture: “Ampliqon 10x Ammonium Buffer, 15 mM”
Tolerance for primer annealing temperatures
10X buffer

ammonium buffer isogen lifescience

Ammonium Buffer: Minimal need for optimisation.

A broad range of Mg2+ concentrations and temperatures results in specific products with high yield (lanes 1.5 – 2.5 and lanes 57 – 66).

Different PCR buffers compositions were analysed for PCR amplification of ENG9.

Ammonium buffer was used in combination with TEMPase polymerase at indicated Mg2+ concentrations or temperatures.
On the left part, Mg2+ dilution series is shown from 0.5 – 4.5 mM Mg2+ at 60°C. The right part shows a temperature gradient from 51 – 66°C at 1.5 mM Mg2+. M: marker.

Standard Buffer

Although we recommend using ammonium buffer, when you already have optimised your PCR protocols, we recommend to keep using the Standard Buffer as the ideal PCR buffer. It is based on potassium and has a high specificity. Nevertheless, it usually needs optimisation regarding primer annealing temperatures and Mg2+ concentrations.

DNA templates with a high purity are desired in combination with this buffer.

Features

Promotes reliable amplification on most DNA templates Picture: “Ampliqon 10x Standard Buffer 15 mM”.
High specificity
10X buffer

Standard Buffer: Optimisation is needed.
In this case, from the PCR buffers we offer, the standard buffer was analysed. A narrow range of Mg2+ concentrations and temperatures results in specific products (lanes 1.5 – 2.0 and lanes 60 – 66).

PCR amplifications of ENG9. Upper buffer was used in combination with TEMPase polymerase at indicated Mg2+ concentrations or temperatures. In the left part, a Mg2+ dilution series is shown from 0.5 – 4.5 mM Mg2+ at 60°C. The right part shows a temperature gradient from 51 – 66°C at 1.5 mM Mg2+. M: marker.

Combination of PCR Buffers

Combination Buffer is a balanced formulation ammonium-potassium PCR buffer which results in high product yield and good specificity. This buffer shows good results on several PCR instruments and is worth testing when setting up a new protocol.

Features

Promotes reliable amplification on most DNA templates
Tolerance toward primer annealing temperature
10X buffer

Combination Buffer: Optimisation is needed.

A narrow range of Mg2+ concentrations and temperatures results in specific products with high yields (lane 1.5 and lanes 60 – 66).

PCR amplifications of ENG9. Different PCR buffers conditions was analysed. Upper buffer was used in combination with TEMPase polymerase at indicated Mg2+ concentrations or temperatures. In the left part, a Mg2+ dilution series is shown from 0.5 – 4.5 mM Mg2+ at 60°C. The right part shows a temperature gradient from 51 – 66°C at 1.5 mM Mg2+. M: marker.

Other PCR buffers

If the previously described buffers do not match to your requirements, we also offer some other buffers that are listed below.

5x PCR Buffer Red
4x GC Buffer l & 4x GC Buffer ll
PCR Grade Water
Betaine Enhancer Solution 5 M

Isogen Life Sciences works on being updated with the most innovative techniques worldwide.

Let us know what your research is about and we can discuss the best buffer for your research!

Tip – Choose the right buffer

We recommend to use ammonium buffer for most PCR applications. High yield can be expected and minimal optimisation is needed regarding annealing temperatures or Mg2+ concentrations.

We recommend using Mg2+ free buffer if you need to optimize your PCR protocol, especially if Mg2+ concentrations lower than 1.5 mM are required in your set-up.

We recommend using detergent free buffers if fluorescent spectrometry is involved in automation and downstream applications.

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Why Isogen Life Science?

At Isogen Life Science, we believe that long term relationships with life science professionals and suppliers are fundamental to solve life science problems. Our curiosity drives us to continually stay involved and to dig deep in dialogue, with both clients and suppliers. It’s these intensive collaborations that enable us to develop tailored, innovative products that push life science’s boundaries. Partner up with us and enjoy our quick support for troubleshooting and questions!