Buffers

The PCR Buffer system

Do you want to get more reliable results? It is known that multiple factors play an important such as the quality of the DNA and the quality of the  instrument itself.  Therefore, choosing the right buffer is essential for creating an optimal environment when performing PCRs.

We offer you several Tris-based solutions that may match your desired PCR assay conditions and applications. The buffers are available in different compositions, and with different components. For instance, we offer buffers with and without Mg2+, Tween 20 or Triton X-100. 

All the buffers are available in 4 options: 15 mM MgCl2, Mg2+ free, Tween free and Mg2+ & Tween free

Contact us to know which one matches better your research 

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TIP – Choose the right buffer

 

 

 

 

The PCR Ammonium buffer includes high yield expectation and a minimal optimisation is needed regarding annealing temperatures or Mg2+ concentrations. For these reasons, we recommend to use ammonium buffer for most PCR applications. Specifically:

  • Use Mg2+ free buffer for the optimization of your PCR protocol, especially if Mg2+ concentrations lower than 1.5 mM are required in your set-up.
  • Use of detergent free buffers if fluorescent spectrometry is involved in automation and downstream applications.

Ammonium Buffer

The ammonium buffer favors a high PCR yield, steady amplification and comes with high specificity. It minimizes the need for optimization of annealing temperatures and/or Mg2+ concentrations. Ammonium buffer also works well when working with difficult templates, such as GC-rich DNA sequences.

Features

  • Promotes amplification on most DNA templates
  • High yield & specificity Picture: “Ampliqon 10x Ammonium Buffer, 15 mM”
  • Tolerance for primer annealing temperatures
  • 10X buffer

ammonium buffer isogen lifescience

Ammonium Buffer: Minimal need for optimisation.

A broad range of Mg2+ concentrations and temperatures results in specific products with high yield (lanes 1.5 – 2.5 and lanes 57 – 66).

PCR amplifications of ENG9. Upper buffer was used in combination with TEMPase polymerase at indicated Mg2+ concentrations or temperatures. In the left part, a Mg2+ dilution series is shown from 0.5 – 4.5 mM Mg2+ at 60°C. The right part shows a temperature gradient from 51 – 66°C at 1.5 mM Mg2+. M: marker.

Standard Buffer

Although we recommend using ammonium buffer, when you already have optimised your PCR protocols for this buffer, we recommend to keep using the Standard Buffer.

The buffer is based on potassium and has a high specificity. Nevertheless, it usually needs optimisation regarding primer annealing temperatures and Mg2+ concentrations.

DNA templates with a high purity are desired in combination with this buffer.

Features

  • Promotes reliable amplification on most DNA templates Picture: “Ampliqon 10x Standard Buffer 15 mM”.
  • High specificity
  • 10X buffer

Standard Buffer: Optimisation is needed.
A narrow range of Mg2+ concentrations and temperatures results in specific products (lanes 1.5 – 2.0 and lanes 60 – 66).

PCR amplifications of ENG9. Upper buffer was used in combination with TEMPase polymerase at indicated Mg2+ concentrations or temperatures. In the left part, a Mg2+ dilution series is shown from 0.5 – 4.5 mM Mg2+ at 60°C. The right part shows a temperature gradient from 51 – 66°C at 1.5 mM Mg2+. M: marker.

Combination Buffer

Combination Buffer is a balanced formulation ammonium-potassium PCR buffer which results in high product yield and good specificity. This buffer shows good results on several PCR instruments and is worth testing when setting up a new protocol.

Features

  • Promotes reliable amplification on most DNA templates
  • Tolerance toward primer annealing temperature
  • 10X buffer

Combination Buffer: Optimisation is needed.

A narrow range of Mg2+ concentrations and temperatures results in specific products with high yields (lane 1.5 and lanes 60 – 66).

PCR amplifications of ENG9. Upper buffer was used in combination with TEMPase polymerase at indicated Mg2+ concentrations or temperatures. In the left part, a Mg2+ dilution series is shown from 0.5 – 4.5 mM Mg2+ at 60°C. The right part shows a temperature gradient from 51 – 66°C at 1.5 mM Mg2+. M: marker.

Other buffers

If the previously described buffers do not match to your requirements, we also offer some other buffers that are listed below.

  • 5x PCR Buffer Red

  • 4x GC Buffer l & 4x GC Buffer ll

  • PCR Grade Water

  • Betaine Enhancer Solution 5 M

 

Isogen Life Sciences works on being  updated with the most innovative techniques worldwide.

Let us know what your research is about and we can discuss the best buffer for your research!

Contact us 

PCR buffer system

Tip – Choose the right buffer

We recommend to use ammonium buffer for most PCR applications. High yield can be expected and minimal optimisation is needed regarding annealing temperatures or Mg2+ concentrations.

We recommend using Mg2+ free buffer if you need to optimize your PCR protocol, especially if Mg2+ concentrations lower than 1.5 mM are required in your set-up.

We recommend using detergent free buffers if fluorescent spectrometry is involved in automation and downstream applications.

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