Spike-In RNA Variant Control Mixes (SIRV)

RNA sequencing is used in many research projects as a powerful tool for transcriptome analysis and holds promise for expanding into diagnostic and therapeutic applications. To be analytically valid, a laboratory test must deliver accurate information in a reproducible and robust manner. Markers and controls are the only way to unambiguously determine the reliability of your experiment, and Lexogen Spike-In RNA Variants (SIRVs) enable the evaluation of both RNA-Seq experiment quality and comparability.

  • Universal markers for comparing and monitoring RNA-seq experiments
  • Validate RNA-seq workflows, identify error sources or biases and improve RNA-seq workflows from Library prep to data analysis
  • Compatible with all platforms and with RNA from any organism, down to single cell level

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Spike-in Transcripts in RNA-seq

The proliferation of different RNA-seq platforms and protocols as well as the continuous efforts to translate NGS into clinical diagnostics has created the need for multi-functional spike-in controls. These are integrated and processed with real samples to enable monitoring and comparing key performance parameters (eg. sensitivity, input-output correlation, detection and quantification of transcript variants). The external controls are RNA molecules of known sequence that are added in pre-determined amounts to a sample. They are subjected to the same protocol steps and the endogenous RNA to be separated only at the final step of the data analysis.

The SIRV Isoform and ERCC Molecules

Transcriptomes are complex and comprise several RNA classes with their own properties. Spike-in controls must reflect these to be representative for a given experimental design. The SIRV’s were developed as a family of modules that offer tailored solutions for the control of RNA measurements. The SIRV Isoform module is available on its own (SIRV-Set 1 and SIRV-Set 2) as well as in combination with the ERCC Module (SIRV-Set 3).

SIRV Set Selection Guide

The Spike-In RNA Variants of the isoform and ERCC modules (see Modular Design) are realized as defined mixes, and for some modules, different mixes are available.

Mixes, and combinations thereof, are available in the form of SIRV sets. Table 1 presents a SIRV set selection guide to help you finding the right set of modules and mixes for your application. 

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