SLAMseq Metabollic RNA labelling
SLAMseq Metabolic Labelling kits enable time-resolved measurement of newly synthesized and existing RNA in cultured cells.
This family of kits uses the fast and reliable thiol (SH)-Linked Alkylation for Metabolic Labelling method. This enables identification and quantification of newly synthesized and existing RNA and the monitoring of RNA synthesis and degradation kinetics from the same sample in parallel, without the need for biochemical isolation, meaning that SLAMseq can be readily applied to living cell experiments.
The SLAMseq kits portfolio includes Explorer and Kinetics Modules, which cover the entire metabolic RNA labelling experiment, from optimizing labelling conditions, to labelling of newly synthesized or existing RNA and alkylation for downstream NGS library prep.
Combining SLAMseq with QuantSeq 3’ mRNA-seq or QuantSeq Flex Targeted RNA-Seq kits provides a complete user-friendly solution for high throughput metabolic RNA-seq experiments.
Total RNA from SLAMseq experiments can be used directly in the QuantSeq protocol. Simply isolate total RNA, alkylate with iodoacetamide, prepare libraries and sequence.
Combining SLAMseq with protein modulators or drug treatments enables distinction between primary and secondary target responses. Use SLAMseq to dissect the underlying biological processes of signalling pathways and characterize drug-target responses on the transcriptional level (Muhar, M et al., 2018)
The Catabolic Kinetics module can be used to measure transcript degradation rates. Cells are first grown in S4U-containing medium for a specified time period to label existing RNA. S4U is then replaced by unmodified uridine (U) and RNA is sampled. RNA degradation rates are calculated from the decrease in S4U-labeled RNA over time.
SLAMseq resolves transcript expression dynamics on a transcriptome-wide scale. Individual transcript RNA synthesis and degradation rates can be measured directly.
Distinguish newly synthesized (nascent) RNA from total RNA using the Anabolic Kinetics Module. Profile nascent RNA levels and perform time course 4SU labelling experiments to map RNA synthesis dynamics for individual transcripts.
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