We host the next CE Discussion Day!
The CE Discussion Group is a working group of the Section Analytical Chemistry of the Royal Dutch Chemistry Society. Over the last decades, it has organized annually one or two meetings, that are focused on the practicalities and applications of CE in the broadest sense of the technique.
The meeting has a strong focus on discussion, enabling participants not only to listen but also to ask anything related to CE. The events are both live and livestreamed.
This fall the CE Discussion Group will have another meeting. This edition will be a full day program held on October 14th, at and promoted by Isogen Life Science in Utrecht. Stay connected to know more about the speakers!
What to expect:
- Several scientists excited to share their work and knowledge.
- Plenty of opportunity to discuss and troubleshoot during round table and troubleshooting sessions.
- Network with fellow scientists in your CE community with some drinks.
- Also learn about the latest trends and the newest innovations of other disciplines!
PROGRAM (speakers will be mentioned soon)
09:30 - 10:00 Welcome and Coffee
10:00 - 12:15 Session 1: Technical presentations
- Rapid clone screening of biosimilar candidates using microfluidic CE-MS (Isogen Life Science and CE Discussion Group Introduction).
- (Ruben Cageling, PolPharma and VU): Depletion-zone isotachophoresis (dzITP) of extracellular vesicles: a separation method suitable for biomarker discovery applications that can increase purity over current techniques
- Andrea Capuona (Exit071)
- Panel Discussion: Rob Haselberg, Cari Sänger - de Griend and Martin Donker
12:15 - 13:15 Lunch
13:15 - 14:15 Session 2: Round table discussions
14:15 - 14:30 Coffee
14:30 - 16:00 Session 3: Troubleshooting sessions
- Ann-Katrin Schwenzer (Aalen University): Native CE of mAb proteoforms coupled to MS under native and denaturing SL conditions
- Aurore Boclinville, Liège University: Interest of Imaged Capillary Isoelectric Focusing for the Characterization of Human Papillomavirus Virus-Like Particles
- Xiaoxi Zhang, Thermo Fisher Scientific: Charge heterogeneity analysis of mAb, recombinant protein vaccine and ADC using denatured and native MS.
16:00 - 17:00 Snacks and Drinks
Speakers:
Xiaoxi Zhang, ThermoFisher Scientific
Xiaoxi Zhang (Application Manager, Pharma and Biopharma, Chromatography and Mass Spectrometry Division, Thermo Fisher Scientific), who has 10+ publications/orals/posters in icIEF-MS for charge variant analysis and biotherapeutic characterization since 2021 will be presenting at 14:30 CEST.
Leading the icIEF- MS collaboration with AES, she will be presenting icIEF MS online coupling for charge heterogeneity analysis of mAb, recombinant protein vaccine and ADC using denatured and native MS
Abstract
As a standard analytical method, imaged capillary isoelectric focusing (iCIEF) has become an indispensable tool in therapeutic protein development and manufacturing because of its high analytical throughput, ease of use, fast method development and excellent reproducibility. Recently, iCIEF-MS has attracted much attention to utilize for protein charge variant analysis.
In this study we did online iCIEF-MS under denaturing condition for mAb and recombinant protein vaccine charge variant analysis, and near-native condition to analyze charge heterogeneity of polatuzumab vedotin, a latest-generation cysteine-linked ADC, using CEinfinite iCIEF platform and Thermo Scientific™ Orbitrap Exploris™ 240 mass spectrometry under Thermo Scientific™ Chromeleon™ software control.
