Company: Molecular Oncology and Embryology, Medicine Faculty of the Barcelona University
Project needs: Personalised medicine for treating lung cancer, the most common cause of cancer-related death in the world.
Our solution:Our microscope-in-incubator EzScope enables to visualise the organoids without perturbing incubator conditions, and therefore improving their growth.
The Molecular Oncology Embryology group is a multidisciplinary research group with six current lab members and different clinical collaborators. It is part of the Medicine Faculty of the Barcelona University. It is composed by basic researchers and specialized physicians, focused on translational research in solid and hematological tumors.
The group performs both basic and clinical research with the aim of improving the survival of oncology patients with different types of cancer, including lung and colorectal cancer.
Lung cancer is the most common cause of cancer-related death in the world. There is a need for personalized medicine to make sure each patient receives the drug needed to overcome disease conditions and improve the patient’s health. For this, three-dimensional models are needed to study models that show lung heterogeneity and complexity.
In the last years, lung cancer organoids from stem cells have emerged to mimic original tumors.
The development of models in vitro to study cellular and molecular cancer stem cells is needed to study in-depth tumorigenesis and identify new therapeutic strategies.
The Molecular Oncology research group grows lung tumoroids from patients to study different drug effectiveness. Read in detail about lung organoids, drug screening, and lung tumoroids in the article stated in the right.
Our microscope-in-incubator EzScope is now part of the Molecular Oncology and Embryology group. They are using it to visualize and record the progress of organoids and their reaction in front of different drugs.
They grow lung organoids starting from the patient’s tumor tissue. After mechanical and enzymatic digestions and red blood cell lysis, with its washing and centrifuge steps in between, they achieve cell suspensions which culture in Matrigel.
Isogen Life Science visited the laboratory with a demo instrument, which stayed there for some days to make sure they were feeling comfortable with it. This time period helped researchers to make sure the instrument matched their work.\
The EzScope has been a valuable tool in our laboratory. This imaging microscope for incubators has allowed us to accurately capture the differentiation process of human embryonic lung organoids into alveolospheres-like structures following transfection with IGFBP3 siRNA.
Documenting morphological changes at regular intervals without disrupting the incubator environment has been crucial for our analysis. The ease of use and the quality of the images obtained with the EzScope have improved the productivity of our experiments, enabling continuous and precise monitoring of organoid development.
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