DBdirect™

Direct PCR without DNA extraction

Skip DNA extraction and go straight to PCR.

DBdirect™ PCR mixes enable direct DNA amplification from cells, viruses and biological samples, eliminating the need for a separate DNA extraction or purification step. This simplifies your workflow, reduces hands-on time and helps you get results faster.

Robust PCR performance, made simple

DBdirect™ mixes combine two technologies in one ready-to-use formulation:

  • DBdirect™ technology enables direct PCR from a wide range of biological samples without prior DNA purification.
  • SuperSens technology, based on the inhibitor-resistant DB SuperSens Taq polymerase, provides excellent sensitivity, specificity and robustness, even with challenging samples.

The single-component formulation keeps reaction setup simple while delivering reliable and consistent amplification for applications ranging from routine detection to highly sensitive assays.

Key benefits

  • Direct PCR without DNA extraction
  • High sensitivity and specificity
  • Excellent resistance to PCR inhibitors
  • Reduced hands-on time and simpler workflows
  • Exceptional stability from fridge to bench
  • Easy-to-use, single-component formulation

Effective contamination control with UDG

Choosing the right direct PCR approach

For many sample types, different direct PCR routes can be used successfully. The optimal approach often depends on the number of cells added to the reaction, as high cell loads may inhibit PCR.

Our application note provides recommendations for different sample types and cell inputs, together with the basic reaction setup to help you get started.

Explore the application note to find the recommended workflow for your sample.

Request a quotation

Request a quotation for your selection of laboratory instruments, reagents, kits or consumables. We will get back to you via mail or phone in no more than 24 hours. Promised!

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Contact information

+31 30 6880771
support@isogen-lifescience.com

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Veldzigt 2A
3454 PW De Meern

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