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NanoDrop ND-2000







Productnr.: ND-2000

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Everything You Loved in the NanoDrop 1000, Plus More

The New Thermo Scientific NanoDrop 2000 delivers the same high-quality performance you've come to expect from our full-spectrum UV-Vis instruments, plus enhancements:

  • Fast measurement time of less than five seconds
  • New, innovative software that allows you to create custom methods and provides flexible options to design reports and export your data
  • Improved capability for proteins with low wavelength absorbance, such as peptides at 205 nm
  • Sample volumes as small as 1 µl, which is ideal for precious high concentration samples
  • Higher concentration measurement capability up to 15,000 ng/µl (dsDNA), which eliminates the need to dilute highly concentrated samples

The sample retention system used by the NanoDrop ND-2000 is probably its best feature. Not only does it enable the analysis of extremely small sample volumes (1 microliter), it also eliminates the need for cuvettes and capillaries. This saves the cost of either disposable cuvettes or the time and effort spent in cleaning reusable ones. For busy laboratories, this feature alone can pay for the cost of the ND-2000!

Benefits

  • Direct, easy measurements in less than 5 seconds – just 
    pipette & wipe
  • Full spectral output
  • Measures DNA, RNA (A260) and Protein (A280) 
    concentrations and sample purity (260/280 ratio)
  • Large concentration range (2 ng/µL – 15,000 ng/µL dsDNA) without dilutions
  • Pre-configured methods for common applications such as 
    Nucleic Acid, Protein A280, Microarray, Proteins & Labels, 
    Bradford, BCA, Lowry and Pierce 660nm – and more
  • User-friendly software that includes Custom Methods and 
    data export capabilities
  • Simple self calibration check using control fluid – no 
    instrument adjustments
  • Low-cost operation – no plates or other consumables

Technology in Brief

Surface tension is used to hold a column of liquid sample in place while a measurement is made. This is done by pipetting the sample directly onto one measurement pedestal. A measurement column is then drawn between the ends of two optical fibers to establish the measurement path as shown below. The measurement is made, typically in less than 10 seconds, and the  spectrum and its analysis is shown on the screen of the attached PC and if necessary archived on the PC.

Applications

  • Microarray Cy-dye Measurement:
    Determination of the dye label concentration of microarray samples.
  • Nucleic Acid Concentration and Quality Measurement:
    Determination of concentration and quality of nucleic acid samples in the range from 2-3700 ng/µl without dilution.
  • Spectrophotometry:
    performs full-spectrum UV/Vis spectrophotometry. Safety is improved when analyzing dangerous materials due to the 1 µl sample size.

Features:

Microliter system

Benefits:

  • No cuvettes needed

Specifications

Wavelength range: 190 - 840nm
Bandwidth: 1 nm
Beam: single
Light source: Xenon flash lamp
Wavelength selection: 2048 - element linear silicon CCD array