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Next Generation PCR

NEXTGENPCR (ultra fast pcr) introduced by MBS

NGPCR: An entirely new ultra fast technology for heating and cooling of samples.

Until now PCR instruments have relied on Peltier elements to heat and cool an aluminium or silver block, accommodating 96 or 384 polypropylene tubes, holding PCR-mixes. Using this technology PCR-times are usually between 50 and 150 minutes, for 30 cycle 3-temperature reactions.
Now, using MBS microplates, and MBS proprietary heat transfer technology, ramp rates of more than 1000 degrees per second become possible. Go from melting to annealing in less than 0.1 second. Total reaction time is between 2 and 10 minutes.

Advantages

  1. Ultra Fast: 30 cycles, 3 temperatures in 2 minutes
  2. Complete microplate compatibility
  3. 96-well or 384-well plates, in the same machine. One machine, ALL FORMATS!!
  4. Just 25 Watts consumption

Benefits

Speed

The NEXTGENPCR performs PCR within 2 minutes. Due to instant temperature changing between denaturation, annealing and extension, the only limitation of the speed is the polymerase used.

Throughput

Reducing the PCR time will result in higher sample throughput. Using optimised applications, more than 10 full plate experiments per hour become possible. In the ideal situation this would mean 80 384 well plates per days, resulting in more than 30000 datapoints per day.

Reduced number of instruments

Due to the higher throughput, laboratories can reduce their number of machines needed. This results in lower costs for purchase and maintenance.

Low energy consumption

One machine uses 25 Watts, rather than 500 Watts. The NEXTGENPCR machine is also up to 30 times faster. This results in up to 600 times less energy use per experiment.

Technology

Since the introduction of PCR many people have tried to increase the speed of PCR. Ramp rates go up to 15 degrees per second, and chemistry is getting faster and faster. The NEXTGENPCR device will heat and cool the samples instantly, loosing no time getting the samples on the desired temperature. Ramp rates are an estimated 1000 degrees per second. For the first time, the machine is not the limitation, but the chemistry is.

Wells are formed of very thin polypropylene foil and attached to SBS compliant rigid plastic frames. After filling these wells with PCR mix, all wells are sealed using a proprietary anvil and a standard heat sealer. All plates are shaped to readily integrate into laboratory automation.

When microplates arrive at a temperature zone, the two temperature blocks are brought together and slightly compress the wells. This process mixes the liquid in each well. Together with the large surface to volume ratio this enables a practically instantaneous change of one temperature to the next.

Sharp polypropylene pipette tips enable piercing of even polyester backed foil. A special holder will prevent tips from piercing the bottom of the wells.

MBS microplates are fully compatible with 96-well and 384-well standards.

The frames have all properties, needed for integration in existing laboratory set-ups. Plates are available in 96-well and 384-well. All plates have the same frame, with a polypropylene foil with 96 or 384 wells. 96-well plates may be used with volumes from 5 – 20 or 2-5 microliter. 384-well plates can be used with volumes from 2 – 5 microliter. The MBS NEXTGENPCR machine takes 96-well plates and 384-well plates without any changes to the NEXTGENPCR-machine. All microplates have wells with a wall thickness of less than 50 microns.

The wells are sealed simultaneously, but individually, using a heat sealer and a proprietary anvil. Wells can be sealed with aluminium or polyester backed polypropylene foil. Both foils can be pierced with MBS pipette tips.

For qPCR, wells can be sealed with our clear seal. MBS pipette tips will also pierce this clear seal.

Pipette tips

MBS proprietary pipette tips are available for easy recovery of PCR-products from sealed microplates. These pipette tips are sharp and easily puncture aluminium backed or even polyester backed sealing films.