16S/16S-18S Microbiome analysis

Name: Isogen Life Science B.V.
Address: Veldzigt 2a, De Meern, 3454 PW, NL
Telephone: +31 30 6880771

Depending on your input source, we offer different kits and services.
When looking at the gut microbiome, the 16S Long Read kit or the 16S Read Cloud kit will be the most suited solution.
When working with environmental samples, such as soil or water, the 16S-18S kit will give the best results as this one has been optimized to work with low biomass samples.

Unlike other kits that mostly only target V3 and V4, the Loop Genomics technology enables targeting the entire 16S gene and therefore all 9 variable regions. This enables a far more accurate classification of species down to species level with great confidence.

Ask your questions or request a quote.

With LoopSeq 16S and LoopSeq 16S-18S, the power of single molecule counting is combined to long-read sequencing, resulting in the lowes possible false positive rate and the best species level classification.
The technology allows to sequence each 16S molecule V1-V9 in a sample and to phase each molecule.
In addition, the single-molecule counting technology eliminates PCR bias and reports molecules based on true abundance. This means that even molecules in very low abundance are detected and quantified, giving a true and accurate image of the species in your sample.

The LoopSeq technology enables over 90% of the reads being classified to species level, whereas with the common V3-V4 systems only allow 35-40% of the reads being classified to species level.

16S Profiling Kits and service

16S Microbiome profiling from Mammalian Derived samples
Covering entire 16S rRNA gene and all 9 variable regions
>99% Unique 16S Molecules assigned at species or genus level
Accurate UMI Based quantification
40x lower error rates than standard Illumina workflows

LoopSeq 16S Long Read Kit

Classification and quantification down to species level
Most comprehensive data

Long-Read 16S rRNA Gene sequencing and analysis service

Service includes library prep, sequencing and Data analysis
Resulsts delivered as easy to understand reports and raw data files
Scalable pricing, depending on number of samples (minimal 8 samples)
Highly attractive volume pricing

16S-18S Profiling services

Microbiome profiling from Environmental samples (water, air, soil, surface swabs, …)
Compatible with low biomass samples
Identification of both prokaryotic and eukaryotic microbes
Long reads covering entire 16S (V1-V9) and 18S gene
Accurate UMI based quantification
40x lower error rates than standard

Long-Read 16S-18S Sequencing and Analyses service

Service includes library prep, sequencing and Data analysis
Results delivered as easy to understand reports and raw data files
Scalable pricing, depending on number of samples (minimal 8 samples)
Highly attractive volume pricing

Why Isogen Life Science?

At Isogen Life Science, we believe that long term relationships with life science professionals and suppliers are fundamental to solve life science problems. Our curiosity drives us to continually stay involved and to dig deep in dialogue, with both clients and suppliers. It’s these intensive collaborations that enable us to develop tailored, innovative products that push life science’s boundaries. Partner up with us and enjoy our quick support for troubleshooting and questions!