The Nadia Instrument of Dolomite Bio is a microfluid based platform for single cell analysis or RNA sequencing and guides you through all the relevant scRNA-Seq steps. On a microfluidic chip, thousands of single cells are individually encapsulated together with barcoded oligo beads. Inside each droplet, the cells are lysed (broken open) and their mRNA is captured on a single uniquely barcoded bead. Droplets are collected in the on-chip reservoir.
It can be used for up- and down-stream processes: RT, PCR and NGS on Illumina sequencers. Up to 8 samples can be measured parallel at a time under 20 minutes. Over 50.000 single cells can be captured per cartridge in a single run. Once sequenced Nadia data can be seamlessly analysed by easy to use and affordable bioinformatic pipelines such as dropSeqPipe or Partek Flow.