Single Cell Sequencing & Single Cell RNA Sequencing

Extremely accessible research for Single Cell RNA Sequencing

Isogen Life Science has remarkably been working on finding the most innovative technologies to make Single Cell Research more accessible for researchers and scientists working in fields such as Single Cell analysis, Single Cell RNA Sequencing, Single Nucleic RNA-Sequencing, Plant Protoplast RNA-Sequencing and the distribution of cells in agarose.

This technology is based on a biology droplet technology. It enables the analysis of single cells from heterogenous samples (cultured cells, biopsies, blood, and other tissues) that can be profiled rapidly for gene expression quantification and identification.

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The main benefits of your Single Cell RNA Sequencing and Single Cell analysis:

  • Low cell doublet rates due to gentle cell agitation.
  • Scalable – 1,2,4 to 8 samples simultaneously.
  • No cross contamination - disposable chips and no wetted instrument parts.
  • Maintain transcriptome state with its Temperature control (4-40 0C).
  • Support from cell to data.
  • Agarose encapsulation.
Nadia Instrument

Save time with the measurement of up to 8 samples in <20 mins.

Over 50.000 single cells can be captured per cartridge in a single run. After sequencing processes, the data precedent from The Nadia can be seamlessly analysed simply. The data analysis for Single Cell RNA Sequencing can be performed with easy-to-use and affordable bioinformatic pipelines such as dropSeqPipe or Partek Flow.

The kits that we recommend using with the microfluid-based technology are the RNAdia Reagent Kit . they enable qualitative and easy-to-use cell profiling.

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Biology droplets technology 

The main application for the biology droplets is Single Cell RNA Sequencing, where the mRNA is captured inside the droplet. However, apart from Single Cell RNA Sequencing, it covers a broad range of applications.

The encapsulation of cells in microfluidics enables solutions for high-throughput Single Cell Sequencing thanks to the analysis of thousands of single cells in parallel in a few minutes. The droplet technology enables:

  • The analysis of homogenous samples: with similar cell size and conditions.
  • Each droplet behaves as a microbioreactor.
  • High consistency and reproducibility, just like high-resolution results
  • Save large amounts of reagents: droplets as micro-compartments

Do you want to know more information ab? Do not hesitate to contact us!

Microfluidic-based platform: The Nadia Instrument

 

Affordable Single Cell RNA Sequencing

The Nadia Instrument is a microfluid-based platform for Single Cell RNA Sequencing or analysis. It guides you through all the relevant Single Cell RNA Sequencing steps. On a microfluidic chip, thousands of single cells are individually encapsulated together with barcoded oligo beads. Inside each droplet, the cells are lysed and their mRNA is captured on a single uniquely barcoded bead. Droplets are collected in the on-chip reservoir

 

 

 

 

It is a fully automated, cost-effective high throughput solution for more accessible Single Cell Research. The end-point PCR and Sequencing techniques can be broad: from upstream to downstream processes: real-time PCR, PCR, and NGS (Illumina sequencers).

Do you want to see if it can match your Single Cell RNA Sequencing and Research?

 We offer a demo!

Contact us for more information

Our microfluidic-based technology has a broader range of applications apart from Single Cell RNA Sequencing and analysis.

The main applications that microfluidics covers in the life sciences are classified in the following fields:

  • Genomics study using droplet biology: Capture the genetic information from single cells, multiple cells, or suspension
  • Hydrogels: discover more about our reagents that enable the use of agarose and collagen scaffolds. Hydrogels enable the profiling of antibody-secreting cells, high-throughput screening for metabolic engineering, growth factor encapsulation, and more!
  • Customisation: No need of understanding microfluidics fully to perform or maximize their approach.
  • Lipid nanoparticles: design of mRNA vaccines, drug discovery, cancer immunotherapy, hereditary disease, encapsulation of mRNA etc.

Contact us to know how can we help you on finding your application!

Optimisation of protocols and new applications: The Nadia Innovate

 

Use flexible protocols and develop new applications in Single Cell RNA Sequencing and Research 

Nadia Innovate enables the development of user-defined Single Cell Research protocols and applications. Now, it is possible to transfer developed protocols to the Nadia Instrument for high throughput parallel operation.

Most innovative parameters control: you can now set up your preference in droplet size, droplet frequency, temperature, agitation, and timing.

Are you looking for flexibility in your Single Cell Research? The Nadia Innovate enables you to study a wide variety of cell types and get the optimal Single Cell RNA sequencing. Do not hesitate to contact us for more information.

Benefits of the Nadia Innovate

  • Creation of new applications & protocol optimization
  • Real-time droplet formation because of the speed microscope and built-in camera.
  • Compatible with a broad range of buffer compositions
  • Protocol sharing with other Nadia users

Do you want to see if it can match your Single Cell RNA Sequencing and Research workflow?

We offer a demo!

Contact us for more information

Customer Reviews

Customer

Customer review

Single Cell RNA Seq on head-neck tumour cells

“With the Nadia Instrument for Single Cell RNA-Sequencing we perform research on head and neck tumors. Currently, the response rate of immunotherapy for these tumors is low. We analyze the different anatomical locations, such as the oral cavity, hypopharynx, larynx and oropharynx.  Our goal is to better understand the tumor microenvironment of these tumors and eventually try to improve the response rate of immunotherapy. One approach to better understand the tumor microenvironment is single cell RNA-sequencing, where we study the mRNA content per single cell within the tumor microenvironment.

The Nadia Instrument provides a clear, well written protocol for single cell encapsulation and mRNA library Preparation. Additionally, quick service and support for questions and troubleshooting is provided.”

Request a quotation

Request a quotation for your selection of laboratory instruments, reagents, kits or consumables. We will get back to you via mail or phone in no more than 24 hours. Promised!

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Contact information

+31 30 6880771
support@isogen-lifescience.com

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