CEInfinite AESlytes Carrier Ampholytes
Carrier ampholytes are essential for isoelectric focusing (IEF) separation of proteins. The fundamental requirements of good Carrier ampholytes for IEF applications are good pH linearity, high buffering capacity, small ΔpK (less than 4), good stability, and insignificant influence on the sample.
Since early Carrier ampholytes were developed before the introduction of imaged capillary IEF (iCIEF), the unique requirement of low UV absorbance could not be applied in iCIEF application. In addition, interaction of Carrier ampholytes with protein in gel media and solution phase is different. Therefore, it is advantageous to have Carrier ampholytes for iCIEF with low protein interaction in solution phase for reproducible iCIEF protein separation and with low UV absorbance for minimised electropherogram baseline noise.