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Unmodified Oligos

Available purification options

Salt Free Oligos

  • De-protected oligonucleotides
  • Quality control of each oligo by OD-Measurement and MALDI-TOF MS

PSF Purification(Purity Salt Free)

  • High purity of > 70%
  • Proprietary cartridge purification based on reverse phased chromatography
  • Cleaned from any toxic chemicals and n-x products
  • Quality control of each oligo by Trityl-Monitoring, OD-Measurement and MALDI-TOF MS

HPLC Purification (High Performance Liquid Chromatography)

  • Available in all synthesis scale (including 0.01 µmol scale)
  • High purity of > 80% due to high chromatographic separation
  • Available for all modified and unmodified oligonucleotides
  • Recommended for RNA oligonucleotides
  • Quality control of each oligo by Trityl-Monitoring, OD-Measurement and MALDI-TOF MS or CGE (> 60mers)

PAGE Purification (Polyacrilamide Gel Electrophoresis)

  • Highest available purity for long mers: > 90% purity
  • Recommended for unmodified oligonucleotides > 60mers
  • Quality control of each oligo by Trityl-Monitoring, OD-Measurement and MALDI-TOF MS (< 60mers) or CGE (> 60mers)

Available Synthesis scales and average yields:

Synthesis Scale [µmol] 0,01 0,05 0,20 1,00 10,00
Salt Free

Avg. Yield [OD] 8 12 25 80 600
Avg. Yield [nmol] 40 60 125 400 3000
PSF Purified
Min. Purity > 70%
Avg. Yield [OD] 5 8 15 45 400
Avg. Yield [nmol] 25 40 75 225 2000
HPLC Purified
Min. Purity > 80%
Avg. Yield [OD] 5 7 10 20 200
Avg. Yield [nmol] 25 30 50 100 1000
PAGE Purified
Min. Purity > 90%
Min. Yield [OD] xx 0,5 0,5 xx xx

Length restriction: 5- 120 mer (0.01 µmol scale: 5-50 mer)