DNA/RNA and Protein Sample Preparation

DNA/RNA and Protein Sample Preparation

Isogen Life Science supplies DNA/RNA sample preparation kits. We offer an advanced and comprehensive range of DNA and RNA extraction kits for manually and automated DNA/RNA sample preparation. From sample collection, stabilisation, isolation to purification. Besides, we offer magnetic tools solutions based on QRET technologies (QuickPick™) reagents and consumables.

NEW! No DNA/RNA extraction required - Direct PCR compatible

Smarter DNA/RNA preparation kits

The Invitek sample preparation kits are outstanding because of their advanced production process. The quality of the products is exceedingly high, resulting in the following advantages for you:

  1. Save time with shorter RNA and DNA purification protocols.
  2. Higher DNA yield by longer enzymatic activity for sample lysis.
  3. More intact DNA – less chemical degradation.

Sample collection, preservation and extraction

Saliva, Swabs, and Stool

It is important to guarantee high-quality samples for getting excellent results. Our non-invasible collection tubes for DNA Samples:

  • Cost saving: avoid cooling, and eliminate costs associated with shipping and temperature.
  • Easy and intuitive use.
  • Reliable results: Minimise bias by microbial growth.

Our sample collection and stabiliser kits enables long-term stability of DNA in stool samples (up to 105 days), in saliva samples (up to 1 year). For more information you can have a look at the brochure or contact us!

  • Purification of microbial DNA by combining Stool Collection tubes with DNA stabiliser kits: Read the application note here for combination with Zymo kits, and here for Qiagen kits. 
  • Is freezing necessary for the conservation of microbiome diversity in stool samples? Have a look at the appnote here.

Soil DNA Extraction kit

Save time with our kit because of its short protocol. The kit enables DNA isolation from microorganisms in soil samples. The technology used is spin column technology. For more information, you can have a look at the brochure. 

DNA Clean Up

Our DNA Clean-up is an easy-to-use kit for routine molecular biology applications. 

  • Save time: 7 min protocol for cleaning up PCR and enzymatic reactions and 20 mins for DNA fragment clean-up from agarose gels
  • 95% recovery of fragment sizes between 80 bp and 30 kb.

We do also offer sample preparation for infectious diseasesnucleic acid extraction kits for infectious diseases. 

RTP Technology

With this technology nucleic acids are able to bind in very low salt concentrations which reduces the amount of wash steps in the DNA and RNA isolation process and thereby leading to faster protocols.  

MSB Technology

Minimal salt binding: DNA Purification from reaction mixtures (PCR, cDNA synthesis, restriction enzyme and other enzymatic reactions). DNA fragments (80 bp to 30 kbp) are recovered with very high yields in a final elution volume of 10 µl.

Range of products

A full range of priotein, DNA and RNA purification products and formats, more than 120, for your DNA/RNA application demands. A wide selection of products for high-throughput and automated sample processing. Many products are developed and manufactured in compliance with the Directive 98/79/EC on in vitro diagnostic medical devices (IVD-Directive).

DNA/RNA and Protein purification

By moving particles, not liquids, the QuickPick™ method offers a clear advantage over conventional protein, RNA and DNA isolation methods. Using the QuickPick method, your nucleic acid and protein purification method is faster, simpler and gentle from different sample sources: plants, human tissue, animal tissue, etc. 

Purification solutions                                     

Magnetic beads for DNA/RNA sample preparation

Magnetic protein, DNA and RNA extraction is built on the affinity on the surface of magnetic particles. A suspension of magnetic particles is mixed with a preparation of the target molecule. Once the target is bound to the group, a magnet is used to separate the target-bound particles from the solution. The unbound material can then be washed away from the particles. Depending on the application, the target molecules can, if necessary, be separated from the magnetic particles.

They are developed and selected for optimal performance in a variety of applications. Beads are selected based on the application, sample characteristics and instrument platform used (compatible with KingFisher systems).

Standardised walk-away workflow starting from blood, FFPE or plasma sample to bisulfite conversion of the isolated DNA on the instrument possible. Application fields: Cancer genetics, Genotyping, Plant genotyping, GMO analysis, Pathogen Detetion, Gene Expression Analysis and Cloning experiments. 

Magnetic particle technology has several advantages

  • Fast and simple: Only 10 min hands-on time.
  • Excellent reproducibility and stability through uniform size distribution
  • Superior suspension stability
  • More economical as other separation methods as centrifugation and vacuum DNA, RNA and protein extraction and softer on the sample. 
  • Less contamination because of moving particles instead of liquids. 

QuicPick™ tools

QuickPick DNA reagents are optimised for a range of sample materials and volumes. You can use the same reagents with PickPen manual tools and with the MagRo™ 96-M robotic workstation for high-quality purified DNA. Our QuickPick DNA Fragment purification kit solution provides:

  • Fast and simple purifying DNA fragments from gel or from liquid solutions.
  • Easy and simple way of purifying high-quality plasmid DNA from bacteria.
  • For poly-A(+) mRNA purification from different sample materials and amounts, such as from one cell to 50 mg tissue.

Benefits of QuickPick™ technology

  • Flexible: wide range of sample materials
  • Scalable: easy solution for variable sample volumes
  • Fast DNA and RNA extraction: 96 samples in one hour, with rapid protocols that ensure high-quality products.
  • Short hands-on-time
  • Move particles, not liquids, to avoid time-consuming aspirating and dispensing steps, just like minimising contamination.
  • Reliable: Reproducible results, robust protocols and high-quality purified product

We provide solutions for different sample sources for DNA and RNA. 

 

Different sample sources DNA & RNA Extraction Kits

CanvaxExtraction Kits are designed for a reliable, easy & fast purification of High-Quality/High-Purity Genomic DNA/RNA from 25+ different starting materials, like

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Contact information

+31 30 6880771
support@isogen-lifescience.com

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