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Gloves, what you need to know

Introduction

In your daily lab routine, gloves are used for protecting yourself and your samples . Gloves are such a common product in the laboratory, one might forget what important job they have.
They seem like a standard consumable, but there are big differences in types of gloves, and their purpose in the lab.
Preventing laboratory accidents requires constant awareness, as laboratory workers are exposed to many potential hazards of chemical, biological and radioactive nature. To create a safer working environment, instructions, training and enforcement of laboratory safety policies and Personal Protective Equipment (PPE)are used. PPE has three categories and laboratory gloves are rated in III, the same category as hazmat suits and gas masks.

Does your glove meet the specified requirements?

The packaging of your gloves should display certain symbols and codes to show that you are working with the proper protection for the materials that you are handling. See table Differences between MDD and PPE Cat. III glove testing for an explanation of the symbols and EN codes.

The Glove, how to choose

Gloves are made from different materials for both safety reasons and personal preferences, the materials most used being latex and nitrile. The choice of material should depend on the reagent(s) used and the type of grip and sensitivity needed.

Latex

Latex is a natural rubber with superior elasticity, tensile strength and comfort; but it can cause latex protein allergies. Latex has a good resistance to water miscible substances, weak acids and alkalis within a pH range of 4 – 10, but poor protection for organic solvents, oils and grease.
For more information on latex sensitivity, please download our Latex Sensitivity document.

Nitrile

Nitrile is a synthetic rubber and is commonly used by people suffering from latex allergy. Nitrile has the same material resistances as Latex.
For a detailed overview of our gloves specifications, see the table Glove Specifications.

Protect yourself!

You should be aware that any glove is only a temporary safety solution. Nitrile or Latex gloves are only for splash protection, meaning that once your gloved hand comes into contact with reagents, the reagent will eventually penetrate your glove and make contact with your skin.
You are strongly advised to change gloves when your current set gets contaminated. You will find more information on safetimes with commonly used chemicals in the table Chemical Breakthrough Times.

Request your test sample

Isogen Life Science proudly supplies Starguard gloves, known worldwide for their strength, protection and excellent fit. We would like to challenge you to request a test sample to feel, see and experience the difference a glove can make.

Overview of possibilities

Nitrile

StarGuard® COMFORT

StarGuard® PROTECT

StarGuard® PROTECT+

StarGuard® SENSITIVE

Latex

StarGuard® TOUCH

Differences between MDD and PPE Cat. III glove testing

MDD 93/42/EEC PPE 89/686/EEC Category III PPE Pictogram Performance Ranking Description / Information
Not required EN 420 EN420 pictogram pH value must be between 3.5 and 9.5 pH value. An excessive amount of acid or alkali has been linked to skin dermatitis and may indicate poor process control.
Not required EN 420   Dexterity
EN 455-2 EN 420   Sizing: MDD min. length = 240 mm. PPE Cat. III min length = 220 mm to 270 mm (depending on glove size)
EN 455-3 EN 420 Min. 50 mg/g of rubber Leachable proteins: required for latex gloves only. Requirements for both MDD and PPE are very similar.
EN 455-2 Not required   Minimum requirements before / after ageing:
Latex: 9 N / 6 N
Synthetic: 9 N / 6 N
Force at break: based upon measurement of the force to break a specimen both before and after accelerated ageing (70 °C for 7 days). Measured in Newtons (N). Test not required for PPE.
EN 455-1 EN374-2:2003
Micro-organisms (Penetration)
EN374 pictogram Level 1: AQL of 4.0
Level 2: AQL of 1.5
Level 3: AQL of 0.65
Tested for protection against liquid penetration and micro-organisms. For PPE, freedom from holes is tested by and air and water leak test for Acceptable Quality Level (AQL). MDD, gloves are tested by water leak test only. Both PPE and MDD Gloves must be at least Level 2 (AQL 1.5).
Not required EN374-3:2003
Chemical Permeation
EN374-3 pictogram Level 1: >10 min
Level 2: >30 min
Level 3: >60 min
Level 4: >120 min
Level 5: >240 min
Level 6: >480 min
Chemical Breakthrough
This pictogram is shown only if a breakthrough time of at least 30 mins has been achieved in at least 3 of the 12 chemicals specified for this standard (see Table 2). The code letters of those three chemicals are shown underneath the shield.
EN374-3 pictogram   This pictogram is used if a glove does not pass the (3 out of 12) test explained above, but it does fulfi ll at least Level 2 of the penetration test EN374-2:2003. This highlights the limitations of the chemical barrier properties of thin gauge disposable gloves, emphasising that they are only designed for incidental splashes. The question mark reminds users that it refers to ‘low chemical resistance’ or ‘waterproof’ gloves. However, gloves showing this symbol will often still have excellent resistance to many chemicals other than those on the list of 12 specifi ed. See the Chemical Breakthrough Times Product Data Sheet for Microflex gloves.
Not required EN388:2003
Mechanical
EN388 pictogram Ranked 1 – 4 (A, C, D),
Ranked 1 – 5 (B).
(The higher the number the better the performance)
The pictogram will show the ranking scored for each of the four tests underneath the shield. The four tests are for:
A. Abrasion resistance.
B. Blade Resistance.
C. Tear Resistance.
D. Puncture Resistance.

Glove specifications

    StarGuard® COMFORT StarGuard® PROTECT StarGuard® PROTECT+ StarGuard® SENSITIVE StarGuard® TOUCH
Specifications Gloves per Case 10 x 250 (XL 230) 10 x 100 10 x 50 10 x 200 10 x 100 (XL 90)
Glove Type Nitrile Nitrile Nitrile Nitrile Latex
Type Powder-Free, Ambidextrous, Single Use, Non-Sterile
Colour Light-Blue Violet-Blue Violet-Blue Blue Natural
Cuff Beaded Beaded Extended, Beaded Beaded Beaded
Texture Fingers Fingers Fingers Fingers Fully
Weight (g) 3.5 5.2 11 4.2 6
Length (mm) 245 250 295 240 245
Cuff Thickness (mm) 0.06 0.09 0.12 0.06 0.1
Palm Thickness (mm) 0.07 0.11 0.15 0.07 0.13
Finger Thickness (mm) 0.11 0.18 0.25 0.1 0.16
Minimum Elongation before Aging (%) 500 500 500 500 650
Elongation after Aging (%) 400 400 500 400 600
Tensile Strength before Aging (MPa) 33 30 30 29 22
Tensile Strength after Aging (MPa) 31 29 27 14 20
Force at Break before Aging (N) 7 10 12 7 9
Force at Break after Aging (N) 7 11 14 7 6
Shelf Life (years) 3 3 5 3 4
AQL 0.65 0.65 0.65 1.5 1.5
PPE 89/686/EEC Personal Protective Equipment (PPE) Category III
MDD 93/42/EEC Medical Device Class I
EN420 Compliant, Sizing for special purpose Compliant Compliant, Sizing for special purpose
EN374-2 Level 3 Level 3 Level 3 Level 2 Level 2
EN374-3 / EN16523-1 Splash protection
EN455 Compliant with Parts 1,2,3
ISO 11193-1 Compliant
ISO 21171 Compliant
ASTM F1671 Compliant
ASTM D6124 Compliant

Chemical Breakthrough Times



StarGuard® COMFORT StarGuard® PROTECT StarGuard® PROTECT+ StarGuard® SENSITIVE StarGuard® TOUCH
Chemicals Acetic Acid (50%) 25 50 277 41 5
Acetone 0 0 0 0 0
Acetonitrile (5% in Ethanol) 0 3 5 0 0
Acrylamide (40%) >480 >480 >480 >480 >480
Beta-Mercaptoethanol (<100%) 0 5 11 2 8
Chloroform (1% in Ethanol) 1 2 15 1 1
Cyclohexane (99%) 10 14 135 33 0
Dimethylsulfoxide (>99.5%) 2 5 23 2 18
Ethanol 0 1 19 1 0
Ethidium Bromide (5%) >480 >480 >480 >480 >480
Formaldehyde (37%) >480 >480 >480 >480 <10
Glutaraldehyde (50%) >480 >480 >480 >480 >480
Hydrochloric Acid (36%) 133 222 >480 222 103
Hydrofluoric Acid (40%) 17 13 18 23 2
Iso-Propanol 2 62 57 5 29
Methanol 0 0 17 0 0
n-Heptane 22 11 93 14 0
Nitric Acid (50%) 21 66 183 9 34
Phenol (0.1%) 188 292 >480 >480 1
Phenol (50% in Ethanol) 0 0 6 1 1
Silver Nitrate 0.171 N >480 >480 >480 >480 >480
Sodium Hydroxide (40%) >480 >480 >480 >480 >480
Sodium Hypochlorite, Bleach (5%) >480 >480 >480 >480 >480
Sulfuric Acid (96%) 2 0 22 2 54
Tetrachloroethylene (>99%) 0 1 9 1 0
Toluene 0 0 1 0 0
Trichloroacetic Acid (>99%) 15 30 61 23 10
Disinfectants Sterillium (Alcohol based) 14 21 125 18 9
Distel Laboratory Disinfectant (1:10) >480 >480 >480 >480 421
Chemgene Laboratory Disinfectant (1:20) >480 >480 >480 >480 >480
Virkon Disinfectant (3%) >480 >480 >480 >480 >480
Phagogermyl 26 50 143 40 45
Stokosept® protect (Alcohol based) 16 27 56 21 4

Caution: All tests for determination of breakthrough times were carried out in minutes according to the EN16523-1:2015 and EN374-3:2003 standard under laboratory conditions. The actual conditions at the actual working space might have an impact on the resistance to chemical permeation and can lead to varying chemical breakthrough times. All recommendations and test results are for reference purposes only and are subject to errors and data revision if updated data becomes available. The test results are no substitute for an evaluation carried out by the user. All StarGuard® gloves are non-sterile, ambidextrous and intended for single-use only. Every effort has been made to ensure the content of this publication is correct. STARLAB can not take responsibility for any errors or omissions