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SENSE mRNA-Seq Library Prep Kit for Illumina, Ion Torrent and SOLiD

SENSE is a complete strand-specific mRNA-Seq library prep kit for accurate gene expression profiling, transcriptome sequencing, discovery and quantification of antisense transcripts and overlapping genes.

Superior Strand-Specificity

The strand-displacement stop/ligation technology used in SENSE generates fewer antisense artifacts which can be produced by template-switching in protocols which utilize RNA or cDNA fragmentation. This results in exceptional (>99.9%) strand-specificity and reduced experimental noise, enabling the detection and quantification of antisense transcripts with high confidence.

Rapid Turnaround

NGS-ready libraries can be produced from total RNA samples in under 5 hours with less than 50% hands-on time, allowing RNA extraction, library preparation and quality control to be performed in one day.

All-in-One Solution

No barcodes, nor additional kits or reagents for library amplification, size selection, or purification are required.

Low Amount of Input Total RNA

The typical amount of input total RNA is 10 ng – 2 µg.

Efficient rRNA Elimination

SENSE mRNA-Seq Library Prep includes an integrated poly(A) mRNA selection process which virtually eliminates cytoplasmic ribosomal RNA (< 0.001% of total reads from Universal Human Reference RNA) and removes the need for additional selection or depletion kits, saving you time and money.

Different Sequencing Read Length

For good representation and even coverage of all transcripts in your experiment the library should have a size suitable for the chosen sequencing read length. The size of SENSE libraries for Illumina can be adjusted by simply modulating appropriate buffers during RT/ligation and purification steps.

Compatibility

SENSE mRNA-Seq Library Prep Kits are available for Illumina, Ion Torrent, and SOLiD sequencing platforms.

Simple Multiplexing

SENSE libraries can be multiplexed with very well balanced barcode sequences. Learn more about barcodes for multiplexing on different sequencing platforms.

 

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