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What is your excuse / Safe, easy and painless / Save money and ECL / Increase your yield twice

Fastest PCR clean up... What is your excuse?

An excellent (post) PCR cleanup is important for your sequencing reaction. Misreads in your sequencing reaction can occur when using not fully purified samples. Performing a 100% efficient and trustworthy cleanup, without any sample loss, used to be easier said than done.
Isogen Life Science is happy to introduce the fastest post PCR cleanup method there is with guaranteed 100% recovery of your precious sample.

Read more about the ExoSAP-IT Express

Nowadays, multiple methods for your post-PCR cleanup are available, each with their own advantages. The advantage of ExoSAP-IT over any other product is a guaranteed 100% recovery of your precious samples. For example, compared to filter columns, less input is needed for a perfect sequencing run since no sample is washed away.

Sequencing of a 1 kb PCR product treated with ExoSAP-IT Express. ExoSAP-IT Express treatment prior to sequencing eliminates miscalls and improves sequencing scores (numbers and bars above sequence; >60, probability of error ≤0.0001%). Sequence shown is approximately 400 bases from primer binding site.

With the ExoSAP-IT Express version, the same 100% recovery is maintained as the gold standard ExoSAP-IT, but cleanup time is reduced from 30 minutes to an amazing 5 minutes! The ExoSAP-IT Express enzymatic cleanup method has been designed to minimize errors by reducing your protocol to a single pipetting step, allowing automated or manual processing from a single tube or microtiter well. ExoSAP-IT Express reagent outperforms the competition by ensuring 100% recovery of all amplicon sizes.

Can you find an excuse for not using ExoSAP-It Express for your PCR clean up?
Please contact us if you want to know more or if there actually is an excuse!
Contact us for more information

Safe, easy and painless DNA collection

Stabilize your valuable DNA using our Saliva and Stool collectors and patented stabilization buffers.
By using the Pre-analytical Sample Processing (PSP®) technique, it is possible to stabilize the samples up to 12 months at room temperature, eliminating

  • the risk of infections due to punctures,
  • discomfort,
  • costs on cooled storage, licenses and extensive personnel training.

Do you need reliable stabilization of DNA in stool, saliva or swab samples at room temperature without DNA degradation?
Read more!

Western blotting - from sample to result

Bright proteins for a shiny price

Our Western Blotting substrates cover the whole range of needed signal intensity and duration, from the entry level to the top of the line. Each product is at the top of its market segment for performance and cost-to-performance ratio.

Western Blot Substrates

Chemiluminescence is the Western blot detection method of choice in many protein laboratories, as it provides high sensitivity and convenience for detection with film or digital imaging equipment such as the ProXima.

Advantages:

  • Allows for multiple exposures to be made in order to obtain the best image
  • Blots may reprobed to optimize detection, or to visualize a second protein
  • Detects and quantifies proteins over a wide range of concentrations
  • Yields the greatest sensitivity of any available detection method
  • The linear range of the signal with respect to protein amount exceeds 3 orders of magnitude

Our blotting products cover a wide range of possible signal intensity and duration, from the entry level, to the top of the line. Each product is at the top of its market segment for performance, and cost to performance ratio.

Substrate Signal intensity Protein Abundance Primary Ab dilution Secondary Ab dilution Product number / Pack Size Shiny price
ECL-SUN Standard High 1/100 - 1/5 000 1/1 000 - 1/15 000 XLS063.250 / 2 x 125 ml 1 kit: € 90
2 kits: € 150
Nova 2.0 Medium High 1/500 - 1/5 000 1/20 000 - 1/100 000 XLS071.250 / 2 x 125 ml 1 kit: € 105
2 kits: € 170
EtaC 2.0 High Medium 1/1 000 - 1/15 000 1/25 000 - 1/150 000 XLS070.250 / 2 x 125 ml 1 kit: € 140
2 kits: € 200
EtaC Ultra 2.0 Very High Low 1/5 000 - 1/50 000 1/50 000 - 1/250 000 XLS075.100 / 2 x 50 ml 1 kit: € 250
2 kits: € 420
Supernova Extreme Very low 1/5 000 - 1/100 000 1/100 000 - 1/500 000 XLS3.100 / 2 x 50 ml 1 kit: € 300
2 kits: € 500

Increase your extraction yield twice

Have you always wanted to get a higher efficiency and yield from your DNA/RNA/protein/cells extractions? Use magnetic beads and move your particles instead of the liquids. With the QuickPick™ Tool you can do that.

Magnetic purification is based on coated metallic beads that attract and retain specific target molecules such as RNA, DNA, protein, protein fractions or cells.
Once the target molecules bind to the beads, a magnet is used to separate the target-retained particles from the solution. The other molecules can then be washed away from the particles.
Depending on the application, the target molecules can then, if necessary, be detached from the beads. Compared to the spinning columns, the magnetic purification is a more gentle way for sample processing, without centrifugal force. Because of the gentle processing, you will extract more intact and longer fragments therefore you will have higher quality sample input for your downstream applications.

In conventional or traditional magnetic beads extraction technologies cases, the liquids are added and removed from the particles. The disadvantage is that there will always be some residual volume left in the tube that can cause inhibition of binding efficiency in your downstream process. By moving particles there is no chance of spoilage of your precious beads and attached target.

This is why Isogen Life Science introduces the QuickPick™ Tool: a pipette-like tool with built-in magnet(s) for working with magnetic particles.The design has been optimized to work with a minimum dead volume resulting in higher efficiency and yield from your extraction.

This allows an easier and faster way of processing of your sample compared to repeated centrifugation steps of the spinning columns and conventional magnetic beads method by pipetting all liquids in advance. Additionally, you don’t have to discard your precious unbound sample anymore after the binding step as you would, using conventional methods. The QuickPick tool allows you to recycle your beads for the same sample, to maximize recovery from difficult and complex samples for a higher yield by doing the cycle again.

  Spin Column Beads particle based kit QuickPick tool based kit
Amount of extractions 50 96 96
Average% yield/sample 80 87 100
Average yield/sample 4.9 µg 5.3 µg 6.1 µg
Purity of extraction* < 1.7 < 1.7 > 1.9
*(Ratio 260280 nm corrected by 320nm absorbance)

Automation solutions

The MagRo™ robotic workstation bring the advantages of the QuickPick magnetic particle technology to automated sample purification.
Available in either 8-magnet or 96-magnet versions, and built to open design standards, the MagRo workstation is suited for a wide range of separation and purification tasks. Since the MagRo uses the exact same protocol and chemistry as the manual tool, no revalidation is needed.

Read more about the MagRo workstation