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Using LIZ, JOE or VIC / Skip DNA extraction / PCR cleanup

Are you still using LIZ, JOE or VIC?

We offer competitive alternatives to LIZ, JOE, NED, VIC and PET at a competitive price, as well as a full range of fluorescent dyes for e.g. LightCycler probes.
Moreover, we offer PSF purified oligos (> 70% purity) for the same price as Salt Free.

DNA/RNA Oligonucleotides and qPCR Probes

The success of your projects is determined by the performance of the primers and probes used. Isogen Life Science offers you significant expertise in the synthesis of both DNA and RNA oligonucleotides and Dual Labelled probes for qPCR, offering you high quality products with the best service. We supply better yields than you are used to.
Click here for a full overview of our available fluorescent dyes, or contact us for more information.

 

Unmodified DNA oligos
  Synthesis scale [µmol]
Purification 0.01 0.05 0.20 1.00 10.00
Salt Free Avg. yield [OD] 8 12 25 80 600
Avg. yield [nmol] 40 60 125 400 3000
PSF® purified Avg. yield [OD] 5 8 15 45 400
Avg. yield [nmol] 25 40 75 225 2000
HPLC purified Avg. yield [OD] 5 7 10 20 200
Avg. yield [nmol] 25 30 50 100 1000

 

Dual labelled probes and Molecular Beacons
5’: FAM, TET, JOE, HEX, Cy3, TAMRA, ROX, Texas Red, Cy3.5, Cy5, Cy5.5, Yakima Yellow, ATTO488, 520, 550, 565, 610, 633, 647N, 680
3’: Dabcyl, Eclipse, TAMRA, BHQ1, BHQ2, BBQ650
  Synthesis scale [µmol]
  0.01 0.05 0.20
Min. yield (OD) 1 1.5 3
Avg. yield (OD) 5 8 10
Avg. yield (nmol) 25 40 50

 

LightCycler Probes
Final Amounts available[nmol]: 1.00 3.00 5.00 10.00
Probe length 15 - 45 bases
Acceptor probe labels:
5': LC 610, 640, 670, 705, Cy5, 5.5, ROX, Texas Red
3': Phosphate, Spacer C3
Donor probe: 3'-Fluoresceine

Be more efficient. PCR without DNA extraction

Extraction of DNA is a time-consuming step to get to your final goal. With Versataq it is possible to skip your DNA extraction and go direct to PCR using any type of crude sample.

VersaTaq is a mutant Taq DNA polymerase for Direct PCR from your sample. It is resistant to common PCR inhibitors, allowing you to simply forget about the need for costly and time consuming extraction and purification processes. Go Direct to PCR!

VersaTaq polymerase works perfectly in a wide range of PCR applications:

  • Whole blood (up to 40%)
  • Feces
  • Blood spots on sample collection cards
  • Animal Tissue
  • Plant Tissue
  • Buccal swabs
  • Soil
  • Urine
  • Crude extracts

Interested in knowing more? click here for the product sheet

Would you like to test it and see how easy it is? click here to contact our sales support.

The world’s fastest PCR cleanup

Isogen Life Science offers PCR cleanup kits, which are designed for a fast and efficient removal of impurities from your DNA samples. how fast can you go?

MSB® Spin PCRapace

Highly concentrated, pure DNA fragments and PCR products are isolated from the solution by using a binding elution. Therefore a washing step is not required.

The purification procedure has been optimized to remove salts, enzymes, nucleotides, mineral oil, agarose, ethidium bromide and other impurities from DNA samples.

Advantages:

  • convenient and fast sample processing
  • the most efficient removal of contaminants
  • high recovery rate of PCR products or DNA fragments
  • broad range of fragment sizes 80 bp - 30 kb can be purified

Interested? Click here for the product sheet.